Experiment: H2 McCas with 10 mM (NH4)2S
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04085 and MMP_RS04090 overlap by 23 nucleotides MMP_RS04090 and MMP_RS04095 are separated by 103 nucleotides
MMP_RS04085: MMP_RS04085 - hypothetical protein, at 773,799 to 775,298
_RS04085
MMP_RS04090: MMP_RS04090 - hypothetical protein, at 775,276 to 775,686
_RS04090
MMP_RS04095: MMP_RS04095 - serine protease, at 775,790 to 776,566
_RS04095
Position (kb)
775
776 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 774.277 kb on - strand, within MMP_RS04085 at 774.277 kb on - strand, within MMP_RS04085 at 774.283 kb on - strand, within MMP_RS04085 at 774.283 kb on - strand, within MMP_RS04085 at 774.284 kb on + strand, within MMP_RS04085 at 774.284 kb on + strand, within MMP_RS04085 at 774.295 kb on - strand, within MMP_RS04085 at 774.296 kb on + strand, within MMP_RS04085 at 774.329 kb on - strand, within MMP_RS04085 at 774.563 kb on - strand, within MMP_RS04085 at 774.582 kb on - strand, within MMP_RS04085 at 774.615 kb on - strand, within MMP_RS04085 at 774.655 kb on - strand, within MMP_RS04085 at 774.732 kb on - strand, within MMP_RS04085 at 774.857 kb on - strand, within MMP_RS04085 at 774.887 kb on - strand, within MMP_RS04085 at 774.890 kb on + strand, within MMP_RS04085 at 774.918 kb on - strand, within MMP_RS04085 at 774.918 kb on - strand, within MMP_RS04085 at 774.918 kb on - strand, within MMP_RS04085 at 774.918 kb on - strand, within MMP_RS04085 at 774.918 kb on - strand, within MMP_RS04085 at 774.918 kb on - strand, within MMP_RS04085 at 774.919 kb on + strand, within MMP_RS04085 at 774.919 kb on + strand, within MMP_RS04085 at 774.919 kb on + strand, within MMP_RS04085 at 774.920 kb on - strand, within MMP_RS04085 at 774.920 kb on - strand, within MMP_RS04085 at 774.959 kb on - strand, within MMP_RS04085 at 774.967 kb on - strand, within MMP_RS04085 at 774.973 kb on + strand, within MMP_RS04085 at 774.975 kb on + strand, within MMP_RS04085 at 774.998 kb on - strand, within MMP_RS04085 at 775.175 kb on + strand at 775.196 kb on - strand at 775.196 kb on - strand at 775.201 kb on - strand at 775.286 kb on - strand at 775.369 kb on - strand, within MMP_RS04090 at 775.448 kb on - strand, within MMP_RS04090 at 775.449 kb on + strand, within MMP_RS04090 at 775.600 kb on + strand, within MMP_RS04090 at 775.605 kb on - strand, within MMP_RS04090 at 775.670 kb on - strand at 775.684 kb on - strand at 775.685 kb on + strand at 775.689 kb on - strand at 775.717 kb on + strand at 775.757 kb on - strand at 775.757 kb on - strand at 775.761 kb on - strand at 775.774 kb on + strand at 775.785 kb on - strand at 775.786 kb on + strand at 775.802 kb on + strand at 776.027 kb on - strand, within MMP_RS04095 at 776.027 kb on - strand, within MMP_RS04095 at 776.028 kb on + strand, within MMP_RS04095 at 776.031 kb on - strand, within MMP_RS04095 at 776.070 kb on - strand, within MMP_RS04095 at 776.071 kb on + strand, within MMP_RS04095 at 776.073 kb on - strand, within MMP_RS04095 at 776.114 kb on + strand, within MMP_RS04095 at 776.140 kb on + strand, within MMP_RS04095 at 776.217 kb on - strand, within MMP_RS04095 at 776.218 kb on + strand, within MMP_RS04095 at 776.218 kb on + strand, within MMP_RS04095 at 776.239 kb on - strand, within MMP_RS04095 at 776.260 kb on - strand, within MMP_RS04095 at 776.290 kb on - strand, within MMP_RS04095 at 776.296 kb on - strand, within MMP_RS04095 at 776.297 kb on + strand, within MMP_RS04095 at 776.325 kb on - strand, within MMP_RS04095 at 776.326 kb on + strand, within MMP_RS04095 at 776.326 kb on + strand, within MMP_RS04095 at 776.346 kb on + strand, within MMP_RS04095 at 776.368 kb on - strand, within MMP_RS04095 at 776.400 kb on - strand, within MMP_RS04095 at 776.400 kb on - strand, within MMP_RS04095 at 776.510 kb on + strand at 776.522 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM (NH4)2S remove 774,277 - MMP_RS04085 0.32 -0.0 774,277 - MMP_RS04085 0.32 +0.1 774,283 - MMP_RS04085 0.32 -0.1 774,283 - MMP_RS04085 0.32 -0.1 774,284 + MMP_RS04085 0.32 +0.3 774,284 + MMP_RS04085 0.32 -1.2 774,295 - MMP_RS04085 0.33 -0.0 774,296 + MMP_RS04085 0.33 +0.3 774,329 - MMP_RS04085 0.35 +0.3 774,563 - MMP_RS04085 0.51 +0.8 774,582 - MMP_RS04085 0.52 -0.3 774,615 - MMP_RS04085 0.54 +0.1 774,655 - MMP_RS04085 0.57 -0.2 774,732 - MMP_RS04085 0.62 -0.6 774,857 - MMP_RS04085 0.71 -0.4 774,887 - MMP_RS04085 0.73 -0.2 774,890 + MMP_RS04085 0.73 +0.1 774,918 - MMP_RS04085 0.75 +0.2 774,918 - MMP_RS04085 0.75 -0.3 774,918 - MMP_RS04085 0.75 -0.0 774,918 - MMP_RS04085 0.75 +0.2 774,918 - MMP_RS04085 0.75 -0.4 774,918 - MMP_RS04085 0.75 -0.4 774,919 + MMP_RS04085 0.75 +0.5 774,919 + MMP_RS04085 0.75 -0.3 774,919 + MMP_RS04085 0.75 +0.3 774,920 - MMP_RS04085 0.75 -0.6 774,920 - MMP_RS04085 0.75 -0.2 774,959 - MMP_RS04085 0.77 -0.4 774,967 - MMP_RS04085 0.78 -0.9 774,973 + MMP_RS04085 0.78 +0.4 774,975 + MMP_RS04085 0.78 +0.4 774,998 - MMP_RS04085 0.80 +1.0 775,175 + +0.6 775,196 - +0.2 775,196 - +0.5 775,201 - -0.4 775,286 - -0.6 775,369 - MMP_RS04090 0.23 -3.8 775,448 - MMP_RS04090 0.42 -2.6 775,449 + MMP_RS04090 0.42 -3.8 775,600 + MMP_RS04090 0.79 -0.2 775,605 - MMP_RS04090 0.80 -2.0 775,670 - -0.4 775,684 - +0.1 775,685 + -0.4 775,689 - -0.3 775,717 + -1.5 775,757 - +0.4 775,757 - +0.1 775,761 - -0.5 775,774 + -0.4 775,785 - +0.4 775,786 + -1.4 775,802 + -0.3 776,027 - MMP_RS04095 0.31 -0.5 776,027 - MMP_RS04095 0.31 -0.0 776,028 + MMP_RS04095 0.31 -1.5 776,031 - MMP_RS04095 0.31 +0.2 776,070 - MMP_RS04095 0.36 +0.2 776,071 + MMP_RS04095 0.36 +0.1 776,073 - MMP_RS04095 0.36 +0.8 776,114 + MMP_RS04095 0.42 +0.1 776,140 + MMP_RS04095 0.45 -0.0 776,217 - MMP_RS04095 0.55 +0.3 776,218 + MMP_RS04095 0.55 +0.5 776,218 + MMP_RS04095 0.55 +0.5 776,239 - MMP_RS04095 0.58 +0.2 776,260 - MMP_RS04095 0.60 +0.3 776,290 - MMP_RS04095 0.64 +0.5 776,296 - MMP_RS04095 0.65 +0.4 776,297 + MMP_RS04095 0.65 +0.8 776,325 - MMP_RS04095 0.69 -0.4 776,326 + MMP_RS04095 0.69 -1.5 776,326 + MMP_RS04095 0.69 +0.8 776,346 + MMP_RS04095 0.72 +0.8 776,368 - MMP_RS04095 0.74 +0.7 776,400 - MMP_RS04095 0.79 -0.4 776,400 - MMP_RS04095 0.79 +0.1 776,510 + -0.5 776,522 + -0.1
Or see this region's nucleotide sequence