Experiment: H2 McCas with 10 mM (NH4)2S
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03380 and MMP_RS03385 are separated by 4 nucleotides MMP_RS03385 and MMP_RS03390 are separated by 16 nucleotides MMP_RS03390 and MMP_RS03395 are separated by 212 nucleotides MMP_RS03395 and MMP_RS03400 are separated by 1 nucleotides MMP_RS03400 and MMP_RS03405 are separated by 3 nucleotides
MMP_RS03380: MMP_RS03380 - DUF354 domain-containing protein, at 634,967 to 635,980
_RS03380
MMP_RS03385: MMP_RS03385 - cation diffusion facilitator family transporter, at 635,985 to 636,860
_RS03385
MMP_RS03390: MMP_RS03390 - malate dehydrogenase, at 636,877 to 637,821
_RS03390
MMP_RS03395: MMP_RS03395 - hypothetical protein, at 638,034 to 638,234
_RS03395
MMP_RS03400: MMP_RS03400 - archease, at 638,236 to 638,652
_RS03400
MMP_RS03405: MMP_RS03405 - RNA 3'-terminal phosphate cyclase, at 638,656 to 639,675
_RS03405
Position (kb) 636
637
638 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 635.973 kb on - strand at 635.978 kb on - strand at 636.014 kb on - strand at 636.290 kb on - strand, within MMP_RS03385 at 636.837 kb on + strand at 636.883 kb on + strand at 636.914 kb on + strand at 637.060 kb on + strand, within MMP_RS03390 at 637.095 kb on - strand, within MMP_RS03390 at 637.102 kb on - strand, within MMP_RS03390 at 637.156 kb on - strand, within MMP_RS03390 at 637.352 kb on + strand, within MMP_RS03390 at 637.433 kb on - strand, within MMP_RS03390 at 637.441 kb on - strand, within MMP_RS03390 at 637.469 kb on + strand, within MMP_RS03390 at 637.476 kb on - strand, within MMP_RS03390 at 637.502 kb on + strand, within MMP_RS03390 at 637.582 kb on + strand, within MMP_RS03390 at 637.586 kb on + strand, within MMP_RS03390 at 637.594 kb on + strand, within MMP_RS03390 at 637.821 kb on - strand at 637.836 kb on - strand at 637.837 kb on + strand at 637.841 kb on + strand at 637.864 kb on - strand at 637.866 kb on - strand at 637.866 kb on - strand at 637.867 kb on + strand at 637.870 kb on - strand at 637.870 kb on - strand at 637.871 kb on + strand at 637.871 kb on + strand at 637.872 kb on - strand at 637.873 kb on + strand at 637.873 kb on + strand at 637.874 kb on - strand at 637.874 kb on - strand at 637.887 kb on + strand at 637.919 kb on + strand at 637.922 kb on - strand at 637.923 kb on + strand at 637.927 kb on + strand at 638.002 kb on - strand at 638.002 kb on - strand at 638.006 kb on - strand at 638.054 kb on - strand, within MMP_RS03395 at 638.127 kb on + strand, within MMP_RS03395 at 638.132 kb on - strand, within MMP_RS03395 at 638.212 kb on + strand, within MMP_RS03395 at 638.315 kb on + strand, within MMP_RS03400 at 638.374 kb on - strand, within MMP_RS03400 at 638.375 kb on + strand, within MMP_RS03400 at 638.473 kb on - strand, within MMP_RS03400 at 638.473 kb on - strand, within MMP_RS03400 at 638.647 kb on + strand at 638.648 kb on - strand at 638.653 kb on + strand at 638.677 kb on - strand at 638.677 kb on - strand at 638.677 kb on - strand at 638.677 kb on - strand at 638.677 kb on - strand at 638.677 kb on - strand at 638.751 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM (NH4)2S remove 635,973 - -0.7 635,978 - +0.2 636,014 - +0.6 636,290 - MMP_RS03385 0.35 -1.0 636,837 + -0.2 636,883 + +0.1 636,914 + -2.0 637,060 + MMP_RS03390 0.19 -1.9 637,095 - MMP_RS03390 0.23 -1.5 637,102 - MMP_RS03390 0.24 -0.8 637,156 - MMP_RS03390 0.30 -0.7 637,352 + MMP_RS03390 0.50 -1.6 637,433 - MMP_RS03390 0.59 -1.6 637,441 - MMP_RS03390 0.60 -1.5 637,469 + MMP_RS03390 0.63 -2.1 637,476 - MMP_RS03390 0.63 -2.1 637,502 + MMP_RS03390 0.66 -3.0 637,582 + MMP_RS03390 0.75 -2.1 637,586 + MMP_RS03390 0.75 -3.4 637,594 + MMP_RS03390 0.76 -0.5 637,821 - -0.9 637,836 - -1.0 637,837 + -1.2 637,841 + -0.9 637,864 - -0.1 637,866 - -0.0 637,866 - -1.4 637,867 + -1.5 637,870 - -0.4 637,870 - -1.9 637,871 + -0.6 637,871 + -2.4 637,872 - -0.6 637,873 + -0.0 637,873 + -0.1 637,874 - +0.0 637,874 - +0.9 637,887 + +0.2 637,919 + +0.1 637,922 - -0.4 637,923 + -0.0 637,927 + +0.0 638,002 - +0.3 638,002 - +0.5 638,006 - +0.4 638,054 - MMP_RS03395 0.10 -0.0 638,127 + MMP_RS03395 0.46 +0.2 638,132 - MMP_RS03395 0.49 +0.3 638,212 + MMP_RS03395 0.89 +0.4 638,315 + MMP_RS03400 0.19 -3.0 638,374 - MMP_RS03400 0.33 -3.3 638,375 + MMP_RS03400 0.33 -4.2 638,473 - MMP_RS03400 0.57 -1.9 638,473 - MMP_RS03400 0.57 -2.8 638,647 + -3.0 638,648 - -3.6 638,653 + -1.2 638,677 - +0.3 638,677 - -0.9 638,677 - -0.4 638,677 - -0.1 638,677 - -0.1 638,677 - -0.7 638,751 + +0.7
Or see this region's nucleotide sequence