Experiment: H2 McCas with 10 mM (NH4)2S
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02895 and MMP_RS02900 are separated by 75 nucleotides MMP_RS02900 and MMP_RS02905 are separated by 9 nucleotides MMP_RS02905 and MMP_RS02910 are separated by 170 nucleotides
MMP_RS02895: MMP_RS02895 - DHH family phosphoesterase, at 551,491 to 552,786
_RS02895
MMP_RS02900: MMP_RS02900 - imidazoleglycerol-phosphate dehydratase HisB, at 552,862 to 553,434
_RS02900
MMP_RS02905: MMP_RS02905 - 2-thiouracil desulfurase family protein, at 553,444 to 553,992
_RS02905
MMP_RS02910: MMP_RS02910 - ABC transporter substrate-binding protein, at 554,163 to 554,957
_RS02910
Position (kb) 552
553
554 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 552.050 kb on + strand, within MMP_RS02895 at 552.092 kb on - strand, within MMP_RS02895 at 552.113 kb on + strand, within MMP_RS02895 at 552.131 kb on + strand, within MMP_RS02895 at 552.187 kb on + strand, within MMP_RS02895 at 552.189 kb on + strand, within MMP_RS02895 at 552.300 kb on - strand, within MMP_RS02895 at 552.336 kb on + strand, within MMP_RS02895 at 552.422 kb on - strand, within MMP_RS02895 at 552.425 kb on + strand, within MMP_RS02895 at 552.433 kb on - strand, within MMP_RS02895 at 552.530 kb on - strand, within MMP_RS02895 at 552.828 kb on + strand at 552.829 kb on - strand at 552.829 kb on - strand at 552.830 kb on + strand at 552.832 kb on - strand at 552.833 kb on + strand at 552.833 kb on + strand at 552.834 kb on - strand at 552.918 kb on + strand at 553.313 kb on - strand, within MMP_RS02900 at 553.530 kb on - strand, within MMP_RS02905 at 553.717 kb on + strand, within MMP_RS02905 at 553.737 kb on + strand, within MMP_RS02905 at 553.746 kb on + strand, within MMP_RS02905 at 554.018 kb on - strand at 554.055 kb on - strand at 554.066 kb on - strand at 554.118 kb on - strand at 554.171 kb on - strand at 554.252 kb on - strand, within MMP_RS02910 at 554.294 kb on + strand, within MMP_RS02910
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM (NH4)2S remove 552,050 + MMP_RS02895 0.43 +0.2 552,092 - MMP_RS02895 0.46 +0.8 552,113 + MMP_RS02895 0.48 -0.2 552,131 + MMP_RS02895 0.49 +0.3 552,187 + MMP_RS02895 0.54 +0.9 552,189 + MMP_RS02895 0.54 +0.7 552,300 - MMP_RS02895 0.62 +1.2 552,336 + MMP_RS02895 0.65 -0.5 552,422 - MMP_RS02895 0.72 +0.0 552,425 + MMP_RS02895 0.72 -0.3 552,433 - MMP_RS02895 0.73 -0.6 552,530 - MMP_RS02895 0.80 +0.7 552,828 + -0.3 552,829 - -2.2 552,829 - -2.0 552,830 + +0.0 552,832 - -3.4 552,833 + -0.9 552,833 + +0.4 552,834 - -4.5 552,918 + -2.4 553,313 - MMP_RS02900 0.79 -3.2 553,530 - MMP_RS02905 0.16 +0.3 553,717 + MMP_RS02905 0.50 +0.5 553,737 + MMP_RS02905 0.53 +0.1 553,746 + MMP_RS02905 0.55 +0.4 554,018 - +2.4 554,055 - -0.9 554,066 - +0.2 554,118 - +0.4 554,171 - -2.5 554,252 - MMP_RS02910 0.11 -0.2 554,294 + MMP_RS02910 0.16 +0.9
Or see this region's nucleotide sequence