Experiment: H2 McCas with 10 mM (NH4)2S
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01720 and MMP_RS01725 are separated by 9 nucleotides MMP_RS01725 and MMP_RS01730 are separated by 50 nucleotides MMP_RS01730 and MMP_RS01735 are separated by 66 nucleotides
MMP_RS01720: MMP_RS01720 - replication factor C large subunit, at 318,173 to 319,633
_RS01720
MMP_RS01725: MMP_RS01725 - class I SAM-dependent methyltransferase family protein, at 319,643 to 320,671
_RS01725
MMP_RS01730: MMP_RS01730 - hypothetical protein, at 320,722 to 321,102
_RS01730
MMP_RS01735: MMP_RS01735 - type II glyceraldehyde-3-phosphate dehydrogenase, at 321,169 to 322,191
_RS01735
Position (kb) 319
320
321 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 319.396 kb on + strand, within MMP_RS01720 at 319.656 kb on + strand at 319.684 kb on + strand at 319.786 kb on - strand, within MMP_RS01725 at 319.831 kb on - strand, within MMP_RS01725 at 319.871 kb on + strand, within MMP_RS01725 at 319.985 kb on - strand, within MMP_RS01725 at 319.999 kb on + strand, within MMP_RS01725 at 320.173 kb on + strand, within MMP_RS01725 at 320.432 kb on + strand, within MMP_RS01725 at 320.584 kb on - strand at 320.594 kb on + strand at 320.650 kb on - strand at 320.695 kb on + strand at 320.696 kb on - strand at 320.697 kb on + strand at 320.698 kb on - strand at 320.698 kb on - strand at 320.709 kb on - strand at 320.734 kb on + strand at 320.743 kb on + strand at 320.844 kb on - strand at 321.020 kb on + strand at 321.023 kb on - strand at 321.024 kb on + strand at 321.308 kb on + strand at 321.319 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM (NH4)2S remove 319,396 + MMP_RS01720 0.84 -0.8 319,656 + +0.1 319,684 + -2.8 319,786 - MMP_RS01725 0.14 -0.9 319,831 - MMP_RS01725 0.18 -3.9 319,871 + MMP_RS01725 0.22 -2.5 319,985 - MMP_RS01725 0.33 -3.7 319,999 + MMP_RS01725 0.35 -1.7 320,173 + MMP_RS01725 0.52 -2.4 320,432 + MMP_RS01725 0.77 -3.0 320,584 - -3.4 320,594 + -3.0 320,650 - -2.5 320,695 + -0.4 320,696 - -0.1 320,697 + -1.2 320,698 - +0.2 320,698 - -1.3 320,709 - -0.0 320,734 + -0.5 320,743 + -1.2 320,844 - -3.6 321,020 + -3.1 321,023 - -3.2 321,024 + -2.1 321,308 + -3.0 321,319 - -3.2
Or see this region's nucleotide sequence