Experiment: H2 McCas with 10 mM (NH4)2S
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01695 and MMP_RS01700 are separated by 91 nucleotides MMP_RS01700 and MMP_RS01705 are separated by 77 nucleotides MMP_RS01705 and MMP_RS01710 are separated by 19 nucleotides
MMP_RS01695: MMP_RS01695 - fructose-1 6-bisphosphatase, at 312,964 to 314,115
_RS01695
MMP_RS01700: MMP_RS01700 - dihydroxy-acid dehydratase, at 314,207 to 315,859
_RS01700
MMP_RS01705: MMP_RS01705 - precorrin-2 C(20)-methyltransferase, at 315,937 to 316,623
_RS01705
MMP_RS01710: MMP_RS01710 - shikimate kinase, at 316,643 to 317,494
_RS01710
Position (kb) 314
315
316 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 313.435 kb on - strand at 313.525 kb on - strand at 314.197 kb on - strand at 314.198 kb on + strand at 314.202 kb on - strand at 314.208 kb on - strand at 314.208 kb on - strand at 314.333 kb on - strand at 314.857 kb on - strand, within MMP_RS01700 at 315.361 kb on + strand, within MMP_RS01700 at 315.828 kb on + strand at 315.902 kb on + strand at 315.939 kb on + strand at 315.972 kb on + strand at 316.278 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM (NH4)2S remove 313,435 - -2.0 313,525 - -3.7 314,197 - -0.8 314,198 + +0.4 314,202 - +0.1 314,208 - +0.6 314,208 - +0.0 314,333 - -3.0 314,857 - MMP_RS01700 0.39 -2.2 315,361 + MMP_RS01700 0.70 -1.5 315,828 + -1.6 315,902 + -1.6 315,939 + +0.1 315,972 + -2.5 316,278 - -2.2
Or see this region's nucleotide sequence