Experiment: MoYLS4 media with N2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2461 and DvMF_2462 are separated by 314 nucleotides DvMF_2462 and DvMF_2463 are separated by 44 nucleotides DvMF_2463 and DvMF_2464 are separated by 432 nucleotides
DvMF_2461: DvMF_2461 - fumarate hydratase (RefSeq), at 3,104,594 to 3,105,433
_2461
DvMF_2462: DvMF_2462 - hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit (RefSeq), at 3,105,748 to 3,106,275
_2462
DvMF_2463: DvMF_2463 - hypothetical protein (RefSeq), at 3,106,320 to 3,107,615
_2463
DvMF_2464: DvMF_2464 - hypothetical protein (RefSeq), at 3,108,048 to 3,108,530
_2464
Position (kb)
3106
3107
3108 Strain fitness (log2 ratio)
-1
0
1 at 3105.343 kb on - strand, within DvMF_2461 at 3105.348 kb on + strand, within DvMF_2461 at 3105.555 kb on + strand at 3105.767 kb on + strand at 3105.834 kb on + strand, within DvMF_2462 at 3105.834 kb on + strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.842 kb on - strand, within DvMF_2462 at 3105.868 kb on - strand, within DvMF_2462 at 3105.902 kb on + strand, within DvMF_2462 at 3105.972 kb on - strand, within DvMF_2462 at 3106.013 kb on - strand, within DvMF_2462 at 3106.085 kb on + strand, within DvMF_2462 at 3106.093 kb on - strand, within DvMF_2462 at 3106.311 kb on + strand at 3106.336 kb on + strand at 3106.618 kb on + strand, within DvMF_2463 at 3106.636 kb on + strand, within DvMF_2463 at 3106.636 kb on + strand, within DvMF_2463 at 3106.664 kb on + strand, within DvMF_2463 at 3106.688 kb on - strand, within DvMF_2463 at 3106.703 kb on + strand, within DvMF_2463 at 3106.934 kb on - strand, within DvMF_2463 at 3107.078 kb on + strand, within DvMF_2463 at 3107.101 kb on - strand, within DvMF_2463 at 3107.106 kb on - strand, within DvMF_2463 at 3107.225 kb on - strand, within DvMF_2463 at 3107.663 kb on + strand at 3107.671 kb on - strand at 3107.704 kb on - strand at 3107.877 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 media with N2 remove 3,105,343 - DvMF_2461 0.89 -0.5 3,105,348 + DvMF_2461 0.90 +0.2 3,105,555 + -0.3 3,105,767 + -0.2 3,105,834 + DvMF_2462 0.16 -0.2 3,105,834 + DvMF_2462 0.16 -0.4 3,105,842 - DvMF_2462 0.18 +0.4 3,105,842 - DvMF_2462 0.18 -0.2 3,105,842 - DvMF_2462 0.18 -0.5 3,105,868 - DvMF_2462 0.23 -0.8 3,105,902 + DvMF_2462 0.29 -0.4 3,105,972 - DvMF_2462 0.42 -0.9 3,106,013 - DvMF_2462 0.50 -0.6 3,106,085 + DvMF_2462 0.64 -0.4 3,106,093 - DvMF_2462 0.65 -0.4 3,106,311 + -1.0 3,106,336 + -0.6 3,106,618 + DvMF_2463 0.23 -0.8 3,106,636 + DvMF_2463 0.24 -0.1 3,106,636 + DvMF_2463 0.24 -1.0 3,106,664 + DvMF_2463 0.27 +0.0 3,106,688 - DvMF_2463 0.28 -1.4 3,106,703 + DvMF_2463 0.30 -0.8 3,106,934 - DvMF_2463 0.47 -0.6 3,107,078 + DvMF_2463 0.58 -1.2 3,107,101 - DvMF_2463 0.60 +0.1 3,107,106 - DvMF_2463 0.61 -0.5 3,107,225 - DvMF_2463 0.70 -0.6 3,107,663 + -0.9 3,107,671 - +0.5 3,107,704 - -0.4 3,107,877 - -0.3
Or see this region's nucleotide sequence