Experiment: Time0 for outgrown library 2.0
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2153 and Synpcc7942_2154 are separated by 73 nucleotides Synpcc7942_2154 and Synpcc7942_2155 are separated by 28 nucleotides Synpcc7942_2155 and glnA are separated by 128 nucleotides glnA and Synpcc7942_2157 are separated by 73 nucleotides Synpcc7942_2157 and apcF are separated by 35 nucleotides apcF and Synpcc7942_2159 are separated by 89 nucleotides
Synpcc7942_2153: Synpcc7942_2153 - hypothetical protein, at 2,237,948 to 2,238,427
_2153
Synpcc7942_2154: Synpcc7942_2154 - hypothetical protein, at 2,238,501 to 2,238,704
_2154
Synpcc7942_2155: Synpcc7942_2155 - hypothetical protein, at 2,238,733 to 2,239,176
_2155
Synpcc7942_2156: glnA - L-glutamine synthetase, at 2,239,305 to 2,240,726
glnA
Synpcc7942_2157: Synpcc7942_2157 - hypothetical protein, at 2,240,800 to 2,241,084
_2157
Synpcc7942_2158: apcF - allophycocyanin, beta subunit, at 2,241,120 to 2,241,629
apcF
Synpcc7942_2159: Synpcc7942_2159 - hypothetical protein, at 2,241,719 to 2,242,651
_2159
Position (kb)
2239
2240
2241 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2238.353 kb on + strand, within Synpcc7942_2153 at 2238.464 kb on + strand at 2238.470 kb on + strand at 2238.596 kb on + strand, within Synpcc7942_2154 at 2238.622 kb on + strand, within Synpcc7942_2154 at 2238.668 kb on - strand, within Synpcc7942_2154 at 2238.673 kb on + strand, within Synpcc7942_2154 at 2238.713 kb on - strand at 2238.725 kb on + strand at 2238.729 kb on + strand at 2238.729 kb on + strand at 2238.729 kb on + strand at 2238.730 kb on + strand at 2238.730 kb on + strand at 2238.730 kb on + strand at 2238.730 kb on + strand at 2238.730 kb on + strand at 2238.733 kb on - strand at 2238.735 kb on + strand at 2238.738 kb on - strand at 2238.753 kb on + strand at 2238.755 kb on + strand at 2238.760 kb on + strand at 2238.763 kb on + strand at 2238.763 kb on - strand at 2238.765 kb on + strand at 2238.765 kb on + strand at 2238.765 kb on + strand at 2238.774 kb on + strand at 2238.774 kb on + strand at 2238.818 kb on + strand, within Synpcc7942_2155 at 2238.831 kb on + strand, within Synpcc7942_2155 at 2238.862 kb on + strand, within Synpcc7942_2155 at 2238.862 kb on + strand, within Synpcc7942_2155 at 2238.862 kb on + strand, within Synpcc7942_2155 at 2238.869 kb on + strand, within Synpcc7942_2155 at 2238.870 kb on - strand, within Synpcc7942_2155 at 2238.911 kb on + strand, within Synpcc7942_2155 at 2238.911 kb on + strand, within Synpcc7942_2155 at 2238.919 kb on - strand, within Synpcc7942_2155 at 2238.937 kb on + strand, within Synpcc7942_2155 at 2238.954 kb on + strand, within Synpcc7942_2155 at 2238.954 kb on + strand, within Synpcc7942_2155 at 2238.960 kb on - strand, within Synpcc7942_2155 at 2238.975 kb on + strand, within Synpcc7942_2155 at 2238.975 kb on + strand, within Synpcc7942_2155 at 2238.975 kb on - strand, within Synpcc7942_2155 at 2238.977 kb on + strand, within Synpcc7942_2155 at 2238.983 kb on - strand, within Synpcc7942_2155 at 2238.985 kb on - strand, within Synpcc7942_2155 at 2238.985 kb on - strand, within Synpcc7942_2155 at 2239.028 kb on + strand, within Synpcc7942_2155 at 2239.028 kb on + strand, within Synpcc7942_2155 at 2239.033 kb on - strand, within Synpcc7942_2155 at 2239.034 kb on - strand, within Synpcc7942_2155 at 2239.105 kb on + strand, within Synpcc7942_2155 at 2239.105 kb on + strand, within Synpcc7942_2155 at 2239.115 kb on + strand, within Synpcc7942_2155 at 2239.145 kb on - strand at 2239.156 kb on - strand at 2239.177 kb on - strand at 2239.188 kb on + strand at 2239.221 kb on - strand at 2239.224 kb on + strand at 2239.227 kb on + strand at 2239.230 kb on - strand at 2239.230 kb on - strand at 2239.230 kb on - strand at 2239.251 kb on - strand at 2239.271 kb on + strand at 2239.294 kb on + strand at 2239.312 kb on + strand at 2239.320 kb on - strand at 2239.320 kb on - strand at 2239.382 kb on + strand at 2239.383 kb on - strand at 2239.392 kb on + strand at 2239.429 kb on + strand at 2239.451 kb on + strand, within glnA at 2239.486 kb on - strand, within glnA at 2239.528 kb on - strand, within glnA at 2239.575 kb on + strand, within glnA at 2239.583 kb on - strand, within glnA at 2239.624 kb on - strand, within glnA at 2239.625 kb on - strand, within glnA at 2239.636 kb on - strand, within glnA at 2239.641 kb on - strand, within glnA at 2239.641 kb on - strand, within glnA at 2239.665 kb on - strand, within glnA at 2239.665 kb on - strand, within glnA at 2239.709 kb on + strand, within glnA at 2239.747 kb on + strand, within glnA at 2239.749 kb on - strand, within glnA at 2239.858 kb on - strand, within glnA at 2239.891 kb on - strand, within glnA at 2239.919 kb on - strand, within glnA at 2239.924 kb on - strand, within glnA at 2240.052 kb on + strand, within glnA at 2240.081 kb on - strand, within glnA at 2240.117 kb on - strand, within glnA at 2240.150 kb on - strand, within glnA at 2240.178 kb on + strand, within glnA at 2240.209 kb on - strand, within glnA at 2240.250 kb on + strand, within glnA at 2240.258 kb on - strand, within glnA at 2240.286 kb on - strand, within glnA at 2240.337 kb on + strand, within glnA at 2240.367 kb on + strand, within glnA at 2240.369 kb on + strand, within glnA at 2240.376 kb on - strand, within glnA at 2240.377 kb on - strand, within glnA at 2240.379 kb on + strand, within glnA at 2240.402 kb on + strand, within glnA at 2240.423 kb on + strand, within glnA at 2240.576 kb on - strand, within glnA at 2240.576 kb on - strand, within glnA at 2240.618 kb on + strand at 2240.672 kb on + strand at 2240.680 kb on - strand at 2240.680 kb on - strand at 2240.705 kb on - strand at 2240.716 kb on - strand at 2240.725 kb on - strand at 2240.730 kb on - strand at 2240.756 kb on + strand at 2240.761 kb on + strand at 2240.782 kb on - strand at 2240.806 kb on - strand at 2240.806 kb on - strand at 2240.820 kb on + strand at 2240.882 kb on - strand, within Synpcc7942_2157 at 2240.887 kb on - strand, within Synpcc7942_2157 at 2240.935 kb on + strand, within Synpcc7942_2157 at 2240.945 kb on + strand, within Synpcc7942_2157 at 2240.950 kb on + strand, within Synpcc7942_2157 at 2241.028 kb on + strand, within Synpcc7942_2157 at 2241.028 kb on + strand, within Synpcc7942_2157 at 2241.036 kb on - strand, within Synpcc7942_2157 at 2241.109 kb on - strand at 2241.112 kb on - strand at 2241.151 kb on + strand at 2241.216 kb on - strand, within apcF at 2241.227 kb on - strand, within apcF at 2241.276 kb on - strand, within apcF at 2241.304 kb on - strand, within apcF at 2241.353 kb on - strand at 2241.381 kb on - strand, within apcF at 2241.399 kb on + strand, within apcF at 2241.399 kb on + strand, within apcF at 2241.399 kb on + strand, within apcF at 2241.399 kb on + strand, within apcF at 2241.407 kb on - strand, within apcF at 2241.411 kb on - strand at 2241.414 kb on + strand, within apcF at 2241.422 kb on - strand, within apcF at 2241.465 kb on + strand, within apcF at 2241.473 kb on + strand, within apcF at 2241.493 kb on + strand, within apcF at 2241.493 kb on + strand, within apcF at 2241.493 kb on + strand, within apcF at 2241.511 kb on + strand, within apcF at 2241.516 kb on - strand, within apcF at 2241.551 kb on + strand, within apcF at 2241.559 kb on - strand, within apcF at 2241.563 kb on - strand, within apcF at 2241.568 kb on - strand, within apcF at 2241.596 kb on + strand at 2241.615 kb on - strand at 2241.615 kb on - strand at 2241.623 kb on + strand at 2241.623 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Time0 for outgrown library 2.0 remove 2,238,353 + Synpcc7942_2153 0.84 +1.3 2,238,464 + +0.2 2,238,470 + +1.8 2,238,596 + Synpcc7942_2154 0.47 +0.6 2,238,622 + Synpcc7942_2154 0.59 -1.2 2,238,668 - Synpcc7942_2154 0.82 -0.0 2,238,673 + Synpcc7942_2154 0.84 -3.7 2,238,713 - +0.1 2,238,725 + -0.9 2,238,729 + +1.2 2,238,729 + +0.8 2,238,729 + -0.8 2,238,730 + -1.0 2,238,730 + -0.1 2,238,730 + -1.2 2,238,730 + +1.7 2,238,730 + +0.6 2,238,733 - +0.4 2,238,735 + -2.6 2,238,738 - +0.5 2,238,753 + +1.0 2,238,755 + -0.9 2,238,760 + +0.0 2,238,763 + -1.4 2,238,763 - -1.2 2,238,765 + -3.6 2,238,765 + +0.6 2,238,765 + +1.1 2,238,774 + -1.4 2,238,774 + +1.1 2,238,818 + Synpcc7942_2155 0.19 -1.0 2,238,831 + Synpcc7942_2155 0.22 +1.1 2,238,862 + Synpcc7942_2155 0.29 -0.6 2,238,862 + Synpcc7942_2155 0.29 +0.3 2,238,862 + Synpcc7942_2155 0.29 +2.8 2,238,869 + Synpcc7942_2155 0.31 -0.0 2,238,870 - Synpcc7942_2155 0.31 +0.9 2,238,911 + Synpcc7942_2155 0.40 -0.1 2,238,911 + Synpcc7942_2155 0.40 +0.7 2,238,919 - Synpcc7942_2155 0.42 -1.9 2,238,937 + Synpcc7942_2155 0.46 -3.4 2,238,954 + Synpcc7942_2155 0.50 -2.0 2,238,954 + Synpcc7942_2155 0.50 +0.7 2,238,960 - Synpcc7942_2155 0.51 -0.8 2,238,975 + Synpcc7942_2155 0.55 -0.7 2,238,975 + Synpcc7942_2155 0.55 -0.2 2,238,975 - Synpcc7942_2155 0.55 -1.2 2,238,977 + Synpcc7942_2155 0.55 +0.1 2,238,983 - Synpcc7942_2155 0.56 -0.2 2,238,985 - Synpcc7942_2155 0.57 -1.9 2,238,985 - Synpcc7942_2155 0.57 +0.2 2,239,028 + Synpcc7942_2155 0.66 -0.1 2,239,028 + Synpcc7942_2155 0.66 -0.8 2,239,033 - Synpcc7942_2155 0.68 +0.7 2,239,034 - Synpcc7942_2155 0.68 +0.1 2,239,105 + Synpcc7942_2155 0.84 -2.0 2,239,105 + Synpcc7942_2155 0.84 -2.6 2,239,115 + Synpcc7942_2155 0.86 +0.7 2,239,145 - -0.4 2,239,156 - -1.0 2,239,177 - -0.4 2,239,188 + -3.7 2,239,221 - -0.2 2,239,224 + -1.3 2,239,227 + +0.2 2,239,230 - -0.7 2,239,230 - -0.2 2,239,230 - +0.9 2,239,251 - +0.7 2,239,271 + -0.5 2,239,294 + +1.4 2,239,312 + +0.3 2,239,320 - -1.6 2,239,320 - -0.5 2,239,382 + -1.5 2,239,383 - +0.4 2,239,392 + +1.6 2,239,429 + -0.2 2,239,451 + glnA Synpcc7942_2156 0.10 -1.2 2,239,486 - glnA Synpcc7942_2156 0.13 +0.1 2,239,528 - glnA Synpcc7942_2156 0.16 -0.5 2,239,575 + glnA Synpcc7942_2156 0.19 +1.2 2,239,583 - glnA Synpcc7942_2156 0.20 -0.6 2,239,624 - glnA Synpcc7942_2156 0.22 -1.6 2,239,625 - glnA Synpcc7942_2156 0.23 -0.5 2,239,636 - glnA Synpcc7942_2156 0.23 -2.4 2,239,641 - glnA Synpcc7942_2156 0.24 -0.0 2,239,641 - glnA Synpcc7942_2156 0.24 -0.8 2,239,665 - glnA Synpcc7942_2156 0.25 -1.1 2,239,665 - glnA Synpcc7942_2156 0.25 -0.4 2,239,709 + glnA Synpcc7942_2156 0.28 -0.6 2,239,747 + glnA Synpcc7942_2156 0.31 -0.9 2,239,749 - glnA Synpcc7942_2156 0.31 -0.9 2,239,858 - glnA Synpcc7942_2156 0.39 +1.6 2,239,891 - glnA Synpcc7942_2156 0.41 -2.7 2,239,919 - glnA Synpcc7942_2156 0.43 -0.6 2,239,924 - glnA Synpcc7942_2156 0.44 -0.8 2,240,052 + glnA Synpcc7942_2156 0.53 -0.9 2,240,081 - glnA Synpcc7942_2156 0.55 +1.4 2,240,117 - glnA Synpcc7942_2156 0.57 -1.8 2,240,150 - glnA Synpcc7942_2156 0.59 +2.2 2,240,178 + glnA Synpcc7942_2156 0.61 -0.4 2,240,209 - glnA Synpcc7942_2156 0.64 +0.2 2,240,250 + glnA Synpcc7942_2156 0.66 -0.2 2,240,258 - glnA Synpcc7942_2156 0.67 +0.6 2,240,286 - glnA Synpcc7942_2156 0.69 -2.8 2,240,337 + glnA Synpcc7942_2156 0.73 +0.9 2,240,367 + glnA Synpcc7942_2156 0.75 -1.8 2,240,369 + glnA Synpcc7942_2156 0.75 +0.4 2,240,376 - glnA Synpcc7942_2156 0.75 +0.4 2,240,377 - glnA Synpcc7942_2156 0.75 +0.9 2,240,379 + glnA Synpcc7942_2156 0.76 +1.1 2,240,402 + glnA Synpcc7942_2156 0.77 -0.9 2,240,423 + glnA Synpcc7942_2156 0.79 -0.3 2,240,576 - glnA Synpcc7942_2156 0.89 -1.8 2,240,576 - glnA Synpcc7942_2156 0.89 -0.4 2,240,618 + -0.7 2,240,672 + -0.7 2,240,680 - -0.8 2,240,680 - -0.6 2,240,705 - +0.1 2,240,716 - +1.4 2,240,725 - -0.8 2,240,730 - -0.5 2,240,756 + -3.0 2,240,761 + -2.9 2,240,782 - -2.5 2,240,806 - -0.7 2,240,806 - +0.4 2,240,820 + -1.2 2,240,882 - Synpcc7942_2157 0.29 -0.1 2,240,887 - Synpcc7942_2157 0.31 +1.4 2,240,935 + Synpcc7942_2157 0.47 +1.8 2,240,945 + Synpcc7942_2157 0.51 +0.1 2,240,950 + Synpcc7942_2157 0.53 +0.1 2,241,028 + Synpcc7942_2157 0.80 -0.9 2,241,028 + Synpcc7942_2157 0.80 -1.4 2,241,036 - Synpcc7942_2157 0.83 +0.7 2,241,109 - +2.2 2,241,112 - -2.3 2,241,151 + +1.6 2,241,216 - apcF Synpcc7942_2158 0.19 -1.7 2,241,227 - apcF Synpcc7942_2158 0.21 -1.1 2,241,276 - apcF Synpcc7942_2158 0.31 -1.2 2,241,304 - apcF Synpcc7942_2158 0.36 -0.5 2,241,353 - +0.2 2,241,381 - apcF Synpcc7942_2158 0.51 +1.6 2,241,399 + apcF Synpcc7942_2158 0.55 -1.2 2,241,399 + apcF Synpcc7942_2158 0.55 +1.3 2,241,399 + apcF Synpcc7942_2158 0.55 +1.5 2,241,399 + apcF Synpcc7942_2158 0.55 -1.6 2,241,407 - apcF Synpcc7942_2158 0.56 -1.3 2,241,411 - +0.1 2,241,414 + apcF Synpcc7942_2158 0.58 +0.4 2,241,422 - apcF Synpcc7942_2158 0.59 -1.5 2,241,465 + apcF Synpcc7942_2158 0.68 +1.6 2,241,473 + apcF Synpcc7942_2158 0.69 +1.3 2,241,493 + apcF Synpcc7942_2158 0.73 +0.1 2,241,493 + apcF Synpcc7942_2158 0.73 +1.9 2,241,493 + apcF Synpcc7942_2158 0.73 +0.3 2,241,511 + apcF Synpcc7942_2158 0.77 +0.9 2,241,516 - apcF Synpcc7942_2158 0.78 +1.1 2,241,551 + apcF Synpcc7942_2158 0.85 -0.2 2,241,559 - apcF Synpcc7942_2158 0.86 +0.9 2,241,563 - apcF Synpcc7942_2158 0.87 +0.6 2,241,568 - apcF Synpcc7942_2158 0.88 -1.2 2,241,596 + +1.0 2,241,615 - -0.9 2,241,615 - -0.7 2,241,623 + +3.1 2,241,623 - -1.8
Or see this region's nucleotide sequence