Experiment: Time0 for outgrown library 2.0
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt minD and minE are separated by 14 nucleotides minE and Synpcc7942_0898 overlap by 4 nucleotides Synpcc7942_0898 and tRNA-Thr are separated by 13 nucleotides tRNA-Thr and Synpcc7942_0899 are separated by 95 nucleotides Synpcc7942_0899 and gifA are separated by 144 nucleotides gifA and Synpcc7942_0901 are separated by 136 nucleotides
Synpcc7942_0896: minD - septum site-determining protein MinD, at 906,832 to 907,638
minD
Synpcc7942_0897: minE - cell division topological specificity factor MinE, at 907,653 to 907,928
minE
Synpcc7942_0898: Synpcc7942_0898 - ferredoxin like protein, at 907,925 to 908,317
_0898
Synpcc7942_R0041: tRNA-Thr - tRNA-Thr, at 908,331 to 908,402
tRNA-Thr
Synpcc7942_0899: Synpcc7942_0899 - hypothetical protein, at 908,498 to 909,295
_0899
Synpcc7942_0900: gifA - hypothetical protein, at 909,440 to 909,643
gifA
Synpcc7942_0901: Synpcc7942_0901 - Haloalkane dehalogenase, at 909,780 to 910,676
_0901
Position (kb) 908
909
910 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 907.532 kb on - strand, within minD at 907.547 kb on - strand, within minD at 907.555 kb on + strand, within minD at 907.579 kb on - strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.582 kb on + strand at 907.590 kb on - strand at 907.590 kb on - strand at 907.590 kb on - strand at 907.590 kb on - strand at 907.925 kb on - strand at 907.933 kb on + strand at 908.045 kb on + strand, within Synpcc7942_0898 at 908.045 kb on + strand, within Synpcc7942_0898 at 908.053 kb on - strand, within Synpcc7942_0898 at 908.059 kb on - strand, within Synpcc7942_0898 at 908.097 kb on + strand, within Synpcc7942_0898 at 908.114 kb on + strand, within Synpcc7942_0898 at 908.128 kb on + strand, within Synpcc7942_0898 at 908.134 kb on + strand, within Synpcc7942_0898 at 908.154 kb on + strand, within Synpcc7942_0898 at 908.154 kb on + strand, within Synpcc7942_0898 at 908.171 kb on - strand, within Synpcc7942_0898 at 908.179 kb on - strand, within Synpcc7942_0898 at 908.184 kb on - strand, within Synpcc7942_0898 at 908.211 kb on + strand, within Synpcc7942_0898 at 908.219 kb on - strand, within Synpcc7942_0898 at 908.243 kb on + strand, within Synpcc7942_0898 at 908.272 kb on - strand, within Synpcc7942_0898 at 908.311 kb on + strand at 908.415 kb on - strand at 908.457 kb on + strand at 908.503 kb on - strand at 908.505 kb on + strand at 908.508 kb on + strand at 908.521 kb on - strand at 908.531 kb on - strand at 908.533 kb on + strand at 908.533 kb on + strand at 908.544 kb on + strand at 908.547 kb on - strand at 908.549 kb on - strand at 908.563 kb on + strand at 908.575 kb on + strand at 908.591 kb on - strand, within Synpcc7942_0899 at 908.681 kb on + strand, within Synpcc7942_0899 at 908.681 kb on + strand, within Synpcc7942_0899 at 908.681 kb on + strand, within Synpcc7942_0899 at 908.696 kb on + strand, within Synpcc7942_0899 at 908.755 kb on + strand, within Synpcc7942_0899 at 908.758 kb on + strand, within Synpcc7942_0899 at 908.766 kb on - strand, within Synpcc7942_0899 at 908.846 kb on - strand, within Synpcc7942_0899 at 908.860 kb on + strand, within Synpcc7942_0899 at 908.865 kb on + strand, within Synpcc7942_0899 at 908.868 kb on - strand, within Synpcc7942_0899 at 908.876 kb on + strand, within Synpcc7942_0899 at 908.922 kb on + strand, within Synpcc7942_0899 at 908.922 kb on + strand, within Synpcc7942_0899 at 908.922 kb on + strand, within Synpcc7942_0899 at 908.938 kb on - strand, within Synpcc7942_0899 at 908.955 kb on - strand, within Synpcc7942_0899 at 909.117 kb on + strand, within Synpcc7942_0899 at 909.117 kb on + strand, within Synpcc7942_0899 at 909.120 kb on - strand, within Synpcc7942_0899 at 909.122 kb on + strand, within Synpcc7942_0899 at 909.122 kb on + strand, within Synpcc7942_0899 at 909.125 kb on - strand, within Synpcc7942_0899 at 909.125 kb on - strand, within Synpcc7942_0899 at 909.150 kb on + strand, within Synpcc7942_0899 at 909.165 kb on - strand, within Synpcc7942_0899 at 909.179 kb on - strand, within Synpcc7942_0899 at 909.186 kb on - strand, within Synpcc7942_0899 at 909.213 kb on + strand, within Synpcc7942_0899 at 909.229 kb on + strand at 909.254 kb on + strand at 909.254 kb on + strand at 909.254 kb on + strand at 909.269 kb on + strand at 909.403 kb on + strand at 909.411 kb on - strand at 909.419 kb on + strand at 909.439 kb on - strand at 909.443 kb on + strand at 909.451 kb on - strand at 909.461 kb on - strand, within gifA at 909.464 kb on + strand, within gifA at 909.464 kb on + strand, within gifA at 909.472 kb on - strand, within gifA at 909.500 kb on - strand, within gifA at 909.505 kb on - strand, within gifA at 909.505 kb on - strand, within gifA at 909.505 kb on - strand, within gifA at 909.520 kb on - strand, within gifA at 909.540 kb on + strand, within gifA at 909.614 kb on - strand, within gifA at 909.628 kb on + strand at 909.638 kb on + strand at 909.777 kb on + strand at 909.782 kb on + strand at 909.821 kb on + strand at 909.824 kb on - strand at 909.879 kb on + strand, within Synpcc7942_0901 at 909.883 kb on + strand, within Synpcc7942_0901 at 909.893 kb on + strand, within Synpcc7942_0901 at 909.914 kb on - strand, within Synpcc7942_0901 at 909.939 kb on + strand, within Synpcc7942_0901 at 909.947 kb on - strand, within Synpcc7942_0901 at 909.947 kb on - strand, within Synpcc7942_0901 at 909.953 kb on - strand, within Synpcc7942_0901 at 909.962 kb on + strand, within Synpcc7942_0901 at 909.992 kb on - strand, within Synpcc7942_0901 at 910.002 kb on + strand, within Synpcc7942_0901 at 910.012 kb on + strand, within Synpcc7942_0901 at 910.020 kb on - strand, within Synpcc7942_0901 at 910.023 kb on + strand, within Synpcc7942_0901 at 910.036 kb on + strand, within Synpcc7942_0901 at 910.036 kb on + strand, within Synpcc7942_0901 at 910.044 kb on - strand, within Synpcc7942_0901 at 910.044 kb on - strand, within Synpcc7942_0901 at 910.054 kb on - strand, within Synpcc7942_0901 at 910.063 kb on + strand, within Synpcc7942_0901 at 910.073 kb on - strand, within Synpcc7942_0901 at 910.099 kb on + strand, within Synpcc7942_0901 at 910.104 kb on + strand, within Synpcc7942_0901 at 910.112 kb on - strand, within Synpcc7942_0901 at 910.112 kb on - strand, within Synpcc7942_0901 at 910.118 kb on - strand, within Synpcc7942_0901 at 910.134 kb on + strand, within Synpcc7942_0901 at 910.134 kb on + strand, within Synpcc7942_0901 at 910.134 kb on + strand, within Synpcc7942_0901 at 910.140 kb on + strand, within Synpcc7942_0901 at 910.142 kb on - strand, within Synpcc7942_0901 at 910.142 kb on - strand, within Synpcc7942_0901 at 910.142 kb on - strand, within Synpcc7942_0901 at 910.163 kb on + strand, within Synpcc7942_0901 at 910.163 kb on + strand, within Synpcc7942_0901 at 910.163 kb on + strand, within Synpcc7942_0901 at 910.163 kb on + strand, within Synpcc7942_0901 at 910.163 kb on + strand, within Synpcc7942_0901 at 910.171 kb on + strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.171 kb on - strand, within Synpcc7942_0901 at 910.181 kb on - strand, within Synpcc7942_0901 at 910.183 kb on - strand, within Synpcc7942_0901 at 910.208 kb on + strand, within Synpcc7942_0901 at 910.208 kb on + strand, within Synpcc7942_0901 at 910.211 kb on - strand, within Synpcc7942_0901 at 910.211 kb on - strand, within Synpcc7942_0901 at 910.216 kb on - strand, within Synpcc7942_0901 at 910.216 kb on - strand, within Synpcc7942_0901 at 910.216 kb on - strand, within Synpcc7942_0901 at 910.219 kb on - strand, within Synpcc7942_0901 at 910.220 kb on + strand, within Synpcc7942_0901 at 910.228 kb on - strand, within Synpcc7942_0901 at 910.242 kb on + strand, within Synpcc7942_0901 at 910.268 kb on + strand, within Synpcc7942_0901
Per-strain Table
Position Strand Gene LocusTag Fraction Time0 for outgrown library 2.0 remove 907,532 - minD Synpcc7942_0896 0.87 +0.5 907,547 - minD Synpcc7942_0896 0.89 -0.8 907,555 + minD Synpcc7942_0896 0.90 -1.5 907,579 - -1.4 907,582 + -2.0 907,582 + +0.1 907,582 + -0.6 907,582 + -0.8 907,582 + +0.7 907,582 + +0.3 907,582 + -1.3 907,590 - -1.9 907,590 - -1.0 907,590 - -1.9 907,590 - +1.5 907,925 - +0.9 907,933 + +1.2 908,045 + Synpcc7942_0898 0.31 +1.5 908,045 + Synpcc7942_0898 0.31 +0.2 908,053 - Synpcc7942_0898 0.33 -0.7 908,059 - Synpcc7942_0898 0.34 -0.9 908,097 + Synpcc7942_0898 0.44 -3.4 908,114 + Synpcc7942_0898 0.48 +1.4 908,128 + Synpcc7942_0898 0.52 -1.3 908,134 + Synpcc7942_0898 0.53 +0.2 908,154 + Synpcc7942_0898 0.58 -0.3 908,154 + Synpcc7942_0898 0.58 -1.8 908,171 - Synpcc7942_0898 0.63 -0.9 908,179 - Synpcc7942_0898 0.65 -3.4 908,184 - Synpcc7942_0898 0.66 -0.6 908,211 + Synpcc7942_0898 0.73 +0.5 908,219 - Synpcc7942_0898 0.75 +1.2 908,243 + Synpcc7942_0898 0.81 +1.3 908,272 - Synpcc7942_0898 0.88 -1.4 908,311 + -0.1 908,415 - +0.3 908,457 + -0.5 908,503 - -0.4 908,505 + -0.5 908,508 + -0.5 908,521 - -1.5 908,531 - -1.5 908,533 + +0.9 908,533 + -3.2 908,544 + +1.4 908,547 - -0.9 908,549 - +1.0 908,563 + +0.8 908,575 + +1.8 908,591 - Synpcc7942_0899 0.12 -1.2 908,681 + Synpcc7942_0899 0.23 -0.3 908,681 + Synpcc7942_0899 0.23 -0.5 908,681 + Synpcc7942_0899 0.23 +0.3 908,696 + Synpcc7942_0899 0.25 -2.6 908,755 + Synpcc7942_0899 0.32 -1.5 908,758 + Synpcc7942_0899 0.33 +0.3 908,766 - Synpcc7942_0899 0.34 -0.7 908,846 - Synpcc7942_0899 0.44 -1.3 908,860 + Synpcc7942_0899 0.45 -1.5 908,865 + Synpcc7942_0899 0.46 -0.7 908,868 - Synpcc7942_0899 0.46 +0.3 908,876 + Synpcc7942_0899 0.47 +1.3 908,922 + Synpcc7942_0899 0.53 -0.4 908,922 + Synpcc7942_0899 0.53 -3.1 908,922 + Synpcc7942_0899 0.53 -2.0 908,938 - Synpcc7942_0899 0.55 +1.5 908,955 - Synpcc7942_0899 0.57 -3.3 909,117 + Synpcc7942_0899 0.78 -0.4 909,117 + Synpcc7942_0899 0.78 -1.0 909,120 - Synpcc7942_0899 0.78 +0.6 909,122 + Synpcc7942_0899 0.78 -1.3 909,122 + Synpcc7942_0899 0.78 +0.4 909,125 - Synpcc7942_0899 0.79 -1.6 909,125 - Synpcc7942_0899 0.79 -1.3 909,150 + Synpcc7942_0899 0.82 -0.8 909,165 - Synpcc7942_0899 0.84 -1.3 909,179 - Synpcc7942_0899 0.85 -3.7 909,186 - Synpcc7942_0899 0.86 -0.9 909,213 + Synpcc7942_0899 0.90 +1.5 909,229 + -3.5 909,254 + +0.6 909,254 + -2.1 909,254 + +1.6 909,269 + +0.6 909,403 + +0.9 909,411 - +0.3 909,419 + -1.1 909,439 - +0.1 909,443 + +1.2 909,451 - +2.0 909,461 - gifA Synpcc7942_0900 0.10 -1.0 909,464 + gifA Synpcc7942_0900 0.12 +0.2 909,464 + gifA Synpcc7942_0900 0.12 -2.9 909,472 - gifA Synpcc7942_0900 0.16 -1.8 909,500 - gifA Synpcc7942_0900 0.29 -1.7 909,505 - gifA Synpcc7942_0900 0.32 -2.5 909,505 - gifA Synpcc7942_0900 0.32 +2.9 909,505 - gifA Synpcc7942_0900 0.32 -1.3 909,520 - gifA Synpcc7942_0900 0.39 -0.5 909,540 + gifA Synpcc7942_0900 0.49 -1.0 909,614 - gifA Synpcc7942_0900 0.85 -1.4 909,628 + -2.2 909,638 + -0.6 909,777 + -0.6 909,782 + -0.9 909,821 + -1.9 909,824 - +0.1 909,879 + Synpcc7942_0901 0.11 -0.5 909,883 + Synpcc7942_0901 0.11 -3.6 909,893 + Synpcc7942_0901 0.13 +0.3 909,914 - Synpcc7942_0901 0.15 -2.8 909,939 + Synpcc7942_0901 0.18 +0.2 909,947 - Synpcc7942_0901 0.19 +0.0 909,947 - Synpcc7942_0901 0.19 +0.1 909,953 - Synpcc7942_0901 0.19 -1.1 909,962 + Synpcc7942_0901 0.20 +1.7 909,992 - Synpcc7942_0901 0.24 -0.3 910,002 + Synpcc7942_0901 0.25 +0.1 910,012 + Synpcc7942_0901 0.26 -0.3 910,020 - Synpcc7942_0901 0.27 -1.1 910,023 + Synpcc7942_0901 0.27 +0.6 910,036 + Synpcc7942_0901 0.29 +2.2 910,036 + Synpcc7942_0901 0.29 +0.5 910,044 - Synpcc7942_0901 0.29 -2.0 910,044 - Synpcc7942_0901 0.29 -2.0 910,054 - Synpcc7942_0901 0.31 -1.1 910,063 + Synpcc7942_0901 0.32 +0.9 910,073 - Synpcc7942_0901 0.33 -0.5 910,099 + Synpcc7942_0901 0.36 +1.1 910,104 + Synpcc7942_0901 0.36 -0.4 910,112 - Synpcc7942_0901 0.37 -0.1 910,112 - Synpcc7942_0901 0.37 -1.8 910,118 - Synpcc7942_0901 0.38 -1.5 910,134 + Synpcc7942_0901 0.39 -1.2 910,134 + Synpcc7942_0901 0.39 +0.9 910,134 + Synpcc7942_0901 0.39 +0.6 910,140 + Synpcc7942_0901 0.40 -4.0 910,142 - Synpcc7942_0901 0.40 -0.9 910,142 - Synpcc7942_0901 0.40 -1.3 910,142 - Synpcc7942_0901 0.40 -0.9 910,163 + Synpcc7942_0901 0.43 -1.9 910,163 + Synpcc7942_0901 0.43 +0.8 910,163 + Synpcc7942_0901 0.43 +1.5 910,163 + Synpcc7942_0901 0.43 -0.1 910,163 + Synpcc7942_0901 0.43 -1.2 910,171 + Synpcc7942_0901 0.44 +0.7 910,171 - Synpcc7942_0901 0.44 +0.4 910,171 - Synpcc7942_0901 0.44 -1.2 910,171 - Synpcc7942_0901 0.44 -2.0 910,171 - Synpcc7942_0901 0.44 +0.7 910,171 - Synpcc7942_0901 0.44 +0.9 910,171 - Synpcc7942_0901 0.44 +0.6 910,181 - Synpcc7942_0901 0.45 -0.1 910,183 - Synpcc7942_0901 0.45 +0.2 910,208 + Synpcc7942_0901 0.48 +0.3 910,208 + Synpcc7942_0901 0.48 -1.6 910,211 - Synpcc7942_0901 0.48 +0.6 910,211 - Synpcc7942_0901 0.48 +1.2 910,216 - Synpcc7942_0901 0.49 -0.9 910,216 - Synpcc7942_0901 0.49 -0.1 910,216 - Synpcc7942_0901 0.49 -0.5 910,219 - Synpcc7942_0901 0.49 -1.3 910,220 + Synpcc7942_0901 0.49 -1.0 910,228 - Synpcc7942_0901 0.50 -0.3 910,242 + Synpcc7942_0901 0.52 +1.7 910,268 + Synpcc7942_0901 0.54 -0.2
Or see this region's nucleotide sequence