Experiment: Time0 for outgrown library 2.0
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrH and frr overlap by 14 nucleotides frr and Synpcc7942_0508 are separated by 6 nucleotides Synpcc7942_0508 and Synpcc7942_0509 are separated by 38 nucleotides Synpcc7942_0509 and serB overlap by 4 nucleotides
Synpcc7942_0506: pyrH - uridylate kinase, at 492,988 to 493,749
pyrH
Synpcc7942_0507: frr - ribosome recycling factor, at 493,736 to 494,284
frr
Synpcc7942_0508: Synpcc7942_0508 - geranylgeranyl reductase, at 494,291 to 495,421
_0508
Synpcc7942_0509: Synpcc7942_0509 - hypothetical protein, at 495,460 to 495,933
_0509
Synpcc7942_0510: serB - 2-hydroxy-3-keto-5-methylthiopentenyl-1- phosphate phosphatase, at 495,930 to 496,580
serB
Position (kb) 494
495
496 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 494.286 kb on - strand at 494.286 kb on - strand at 494.299 kb on - strand at 494.318 kb on + strand at 494.354 kb on - strand at 494.369 kb on - strand at 494.390 kb on + strand at 494.394 kb on - strand at 494.402 kb on + strand at 494.402 kb on + strand at 494.415 kb on - strand, within Synpcc7942_0508 at 494.420 kb on - strand, within Synpcc7942_0508 at 494.468 kb on + strand, within Synpcc7942_0508 at 494.484 kb on - strand, within Synpcc7942_0508 at 494.556 kb on + strand, within Synpcc7942_0508 at 494.559 kb on - strand, within Synpcc7942_0508 at 494.559 kb on - strand, within Synpcc7942_0508 at 494.628 kb on - strand, within Synpcc7942_0508 at 494.646 kb on - strand, within Synpcc7942_0508 at 494.646 kb on - strand, within Synpcc7942_0508 at 494.674 kb on + strand, within Synpcc7942_0508 at 494.692 kb on + strand, within Synpcc7942_0508 at 494.697 kb on + strand, within Synpcc7942_0508 at 494.709 kb on + strand, within Synpcc7942_0508 at 494.738 kb on - strand, within Synpcc7942_0508 at 494.751 kb on + strand, within Synpcc7942_0508 at 494.753 kb on - strand, within Synpcc7942_0508 at 494.791 kb on - strand, within Synpcc7942_0508 at 494.804 kb on - strand, within Synpcc7942_0508 at 494.809 kb on - strand, within Synpcc7942_0508 at 494.824 kb on + strand, within Synpcc7942_0508 at 494.828 kb on - strand, within Synpcc7942_0508 at 494.832 kb on - strand, within Synpcc7942_0508 at 494.832 kb on - strand, within Synpcc7942_0508 at 494.837 kb on - strand, within Synpcc7942_0508 at 494.916 kb on - strand, within Synpcc7942_0508 at 494.916 kb on - strand, within Synpcc7942_0508 at 494.941 kb on + strand, within Synpcc7942_0508 at 494.981 kb on - strand, within Synpcc7942_0508 at 495.034 kb on + strand, within Synpcc7942_0508 at 495.041 kb on + strand, within Synpcc7942_0508 at 495.041 kb on + strand, within Synpcc7942_0508 at 495.041 kb on + strand, within Synpcc7942_0508 at 495.042 kb on - strand, within Synpcc7942_0508 at 495.042 kb on - strand, within Synpcc7942_0508 at 495.049 kb on - strand, within Synpcc7942_0508 at 495.049 kb on - strand, within Synpcc7942_0508 at 495.049 kb on - strand, within Synpcc7942_0508 at 495.083 kb on + strand, within Synpcc7942_0508 at 495.091 kb on - strand, within Synpcc7942_0508 at 495.095 kb on + strand, within Synpcc7942_0508 at 495.098 kb on - strand, within Synpcc7942_0508 at 495.102 kb on - strand, within Synpcc7942_0508 at 495.102 kb on - strand, within Synpcc7942_0508 at 495.111 kb on + strand, within Synpcc7942_0508 at 495.127 kb on + strand, within Synpcc7942_0508 at 495.127 kb on + strand, within Synpcc7942_0508 at 495.135 kb on - strand, within Synpcc7942_0508 at 495.194 kb on + strand at 495.194 kb on + strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.202 kb on - strand, within Synpcc7942_0508 at 495.217 kb on + strand, within Synpcc7942_0508 at 495.217 kb on + strand, within Synpcc7942_0508 at 495.217 kb on - strand, within Synpcc7942_0508 at 495.229 kb on + strand, within Synpcc7942_0508 at 495.235 kb on + strand, within Synpcc7942_0508 at 495.235 kb on + strand, within Synpcc7942_0508 at 495.235 kb on + strand, within Synpcc7942_0508 at 495.235 kb on + strand, within Synpcc7942_0508 at 495.235 kb on + strand, within Synpcc7942_0508 at 495.236 kb on + strand, within Synpcc7942_0508 at 495.243 kb on - strand, within Synpcc7942_0508 at 495.243 kb on - strand at 495.243 kb on - strand, within Synpcc7942_0508 at 495.243 kb on - strand, within Synpcc7942_0508 at 495.243 kb on - strand, within Synpcc7942_0508 at 495.272 kb on + strand at 495.282 kb on - strand, within Synpcc7942_0508 at 495.304 kb on - strand, within Synpcc7942_0508 at 495.315 kb on - strand at 495.352 kb on + strand at 495.353 kb on + strand at 495.360 kb on - strand at 495.419 kb on - strand at 495.423 kb on - strand at 495.423 kb on - strand at 495.428 kb on + strand at 495.431 kb on - strand at 495.458 kb on + strand at 495.458 kb on + strand at 495.548 kb on - strand, within Synpcc7942_0509 at 495.555 kb on + strand, within Synpcc7942_0509 at 495.563 kb on - strand, within Synpcc7942_0509 at 495.575 kb on + strand, within Synpcc7942_0509 at 495.592 kb on - strand, within Synpcc7942_0509 at 495.656 kb on + strand, within Synpcc7942_0509 at 495.674 kb on - strand, within Synpcc7942_0509 at 495.680 kb on + strand, within Synpcc7942_0509 at 495.685 kb on + strand, within Synpcc7942_0509 at 495.710 kb on - strand, within Synpcc7942_0509 at 495.722 kb on - strand, within Synpcc7942_0509 at 495.731 kb on - strand, within Synpcc7942_0509 at 495.733 kb on + strand at 495.759 kb on + strand, within Synpcc7942_0509 at 495.766 kb on + strand, within Synpcc7942_0509 at 495.780 kb on - strand, within Synpcc7942_0509 at 495.791 kb on - strand, within Synpcc7942_0509 at 495.829 kb on - strand, within Synpcc7942_0509 at 495.850 kb on + strand, within Synpcc7942_0509 at 495.850 kb on + strand, within Synpcc7942_0509 at 495.858 kb on - strand, within Synpcc7942_0509 at 495.865 kb on + strand, within Synpcc7942_0509 at 495.896 kb on + strand at 495.903 kb on - strand at 495.943 kb on - strand at 495.956 kb on - strand at 495.979 kb on - strand at 495.979 kb on - strand at 495.981 kb on + strand at 496.119 kb on - strand, within serB at 496.148 kb on + strand, within serB at 496.159 kb on + strand, within serB at 496.171 kb on + strand, within serB at 496.174 kb on - strand, within serB at 496.176 kb on + strand, within serB at 496.212 kb on + strand, within serB at 496.213 kb on + strand, within serB at 496.223 kb on - strand, within serB at 496.232 kb on + strand, within serB at 496.250 kb on - strand, within serB at 496.255 kb on + strand, within serB at 496.263 kb on - strand, within serB at 496.264 kb on + strand, within serB at 496.267 kb on - strand, within serB at 496.346 kb on + strand, within serB at 496.351 kb on + strand at 496.351 kb on + strand, within serB at 496.354 kb on - strand, within serB at 496.354 kb on - strand, within serB at 496.354 kb on - strand, within serB at 496.354 kb on - strand, within serB at 496.359 kb on - strand, within serB
Per-strain Table
Position Strand Gene LocusTag Fraction Time0 for outgrown library 2.0 remove 494,286 - -2.1 494,286 - -2.4 494,299 - -3.0 494,318 + +6.3 494,354 - +6.4 494,369 - +2.9 494,390 + +3.5 494,394 - +4.2 494,402 + +4.9 494,402 + +6.2 494,415 - Synpcc7942_0508 0.11 +6.2 494,420 - Synpcc7942_0508 0.11 +5.8 494,468 + Synpcc7942_0508 0.16 +0.4 494,484 - Synpcc7942_0508 0.17 +5.2 494,556 + Synpcc7942_0508 0.23 +4.4 494,559 - Synpcc7942_0508 0.24 +5.6 494,559 - Synpcc7942_0508 0.24 +6.1 494,628 - Synpcc7942_0508 0.30 +5.9 494,646 - Synpcc7942_0508 0.31 +5.3 494,646 - Synpcc7942_0508 0.31 +6.2 494,674 + Synpcc7942_0508 0.34 +5.7 494,692 + Synpcc7942_0508 0.35 +4.8 494,697 + Synpcc7942_0508 0.36 +4.9 494,709 + Synpcc7942_0508 0.37 +5.3 494,738 - Synpcc7942_0508 0.40 +6.0 494,751 + Synpcc7942_0508 0.41 +4.1 494,753 - Synpcc7942_0508 0.41 +5.9 494,791 - Synpcc7942_0508 0.44 +5.7 494,804 - Synpcc7942_0508 0.45 +4.5 494,809 - Synpcc7942_0508 0.46 +4.8 494,824 + Synpcc7942_0508 0.47 +4.8 494,828 - Synpcc7942_0508 0.47 +5.5 494,832 - Synpcc7942_0508 0.48 +3.5 494,832 - Synpcc7942_0508 0.48 +4.8 494,837 - Synpcc7942_0508 0.48 +3.9 494,916 - Synpcc7942_0508 0.55 +5.7 494,916 - Synpcc7942_0508 0.55 +4.5 494,941 + Synpcc7942_0508 0.57 +6.1 494,981 - Synpcc7942_0508 0.61 +4.6 495,034 + Synpcc7942_0508 0.66 +5.0 495,041 + Synpcc7942_0508 0.66 +4.7 495,041 + Synpcc7942_0508 0.66 +3.9 495,041 + Synpcc7942_0508 0.66 +4.0 495,042 - Synpcc7942_0508 0.66 +5.5 495,042 - Synpcc7942_0508 0.66 +6.5 495,049 - Synpcc7942_0508 0.67 +5.2 495,049 - Synpcc7942_0508 0.67 +5.0 495,049 - Synpcc7942_0508 0.67 +5.2 495,083 + Synpcc7942_0508 0.70 +5.6 495,091 - Synpcc7942_0508 0.71 +1.5 495,095 + Synpcc7942_0508 0.71 +5.4 495,098 - Synpcc7942_0508 0.71 +5.4 495,102 - Synpcc7942_0508 0.72 +5.9 495,102 - Synpcc7942_0508 0.72 +5.2 495,111 + Synpcc7942_0508 0.73 +6.0 495,127 + Synpcc7942_0508 0.74 +5.7 495,127 + Synpcc7942_0508 0.74 +4.3 495,135 - Synpcc7942_0508 0.75 +5.1 495,194 + +4.4 495,194 + Synpcc7942_0508 0.80 +3.3 495,202 - Synpcc7942_0508 0.81 +4.9 495,202 - Synpcc7942_0508 0.81 +5.5 495,202 - Synpcc7942_0508 0.81 +5.8 495,202 - Synpcc7942_0508 0.81 +4.2 495,202 - Synpcc7942_0508 0.81 +6.4 495,202 - Synpcc7942_0508 0.81 +6.0 495,202 - Synpcc7942_0508 0.81 +4.6 495,217 + Synpcc7942_0508 0.82 +5.9 495,217 + Synpcc7942_0508 0.82 +3.2 495,217 - Synpcc7942_0508 0.82 +5.4 495,229 + Synpcc7942_0508 0.83 +5.0 495,235 + Synpcc7942_0508 0.83 +5.9 495,235 + Synpcc7942_0508 0.83 +4.3 495,235 + Synpcc7942_0508 0.83 +6.0 495,235 + Synpcc7942_0508 0.83 +4.2 495,235 + Synpcc7942_0508 0.83 +5.6 495,236 + Synpcc7942_0508 0.84 +6.0 495,243 - Synpcc7942_0508 0.84 +4.8 495,243 - +5.2 495,243 - Synpcc7942_0508 0.84 +4.9 495,243 - Synpcc7942_0508 0.84 +4.8 495,243 - Synpcc7942_0508 0.84 +5.1 495,272 + +4.4 495,282 - Synpcc7942_0508 0.88 +3.7 495,304 - Synpcc7942_0508 0.90 +6.1 495,315 - +4.1 495,352 + +3.1 495,353 + +6.1 495,360 - +4.4 495,419 - +5.9 495,423 - -1.4 495,423 - -0.7 495,428 + -0.3 495,431 - -1.0 495,458 + -0.8 495,458 + +0.6 495,548 - Synpcc7942_0509 0.19 -0.9 495,555 + Synpcc7942_0509 0.20 -1.5 495,563 - Synpcc7942_0509 0.22 -0.5 495,575 + Synpcc7942_0509 0.24 -1.2 495,592 - Synpcc7942_0509 0.28 +1.5 495,656 + Synpcc7942_0509 0.41 -2.1 495,674 - Synpcc7942_0509 0.45 +1.0 495,680 + Synpcc7942_0509 0.46 -0.5 495,685 + Synpcc7942_0509 0.47 +0.2 495,710 - Synpcc7942_0509 0.53 +1.3 495,722 - Synpcc7942_0509 0.55 +0.1 495,731 - Synpcc7942_0509 0.57 +0.6 495,733 + -1.4 495,759 + Synpcc7942_0509 0.63 -0.5 495,766 + Synpcc7942_0509 0.65 -2.0 495,780 - Synpcc7942_0509 0.68 -0.1 495,791 - Synpcc7942_0509 0.70 -2.1 495,829 - Synpcc7942_0509 0.78 +0.8 495,850 + Synpcc7942_0509 0.82 -1.8 495,850 + Synpcc7942_0509 0.82 +1.4 495,858 - Synpcc7942_0509 0.84 +0.0 495,865 + Synpcc7942_0509 0.85 -2.1 495,896 + +0.6 495,903 - -2.1 495,943 - -1.4 495,956 - +0.5 495,979 - +0.7 495,979 - -1.2 495,981 + +0.8 496,119 - serB Synpcc7942_0510 0.29 -2.2 496,148 + serB Synpcc7942_0510 0.33 -1.4 496,159 + serB Synpcc7942_0510 0.35 -0.3 496,171 + serB Synpcc7942_0510 0.37 +1.3 496,174 - serB Synpcc7942_0510 0.37 +1.3 496,176 + serB Synpcc7942_0510 0.38 +0.1 496,212 + serB Synpcc7942_0510 0.43 -2.4 496,213 + serB Synpcc7942_0510 0.43 +2.1 496,223 - serB Synpcc7942_0510 0.45 +2.9 496,232 + serB Synpcc7942_0510 0.46 +1.4 496,250 - serB Synpcc7942_0510 0.49 -1.6 496,255 + serB Synpcc7942_0510 0.50 +1.0 496,263 - serB Synpcc7942_0510 0.51 +1.6 496,264 + serB Synpcc7942_0510 0.51 -1.7 496,267 - serB Synpcc7942_0510 0.52 -0.4 496,346 + serB Synpcc7942_0510 0.64 +0.6 496,351 + +1.8 496,351 + serB Synpcc7942_0510 0.65 +3.2 496,354 - serB Synpcc7942_0510 0.65 +1.8 496,354 - serB Synpcc7942_0510 0.65 +0.6 496,354 - serB Synpcc7942_0510 0.65 -0.9 496,354 - serB Synpcc7942_0510 0.65 +1.0 496,359 - serB Synpcc7942_0510 0.66 +0.4
Or see this region's nucleotide sequence