Experiment: Time0 for outgrown library 2.0
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aldA and Synpcc7942_0490 are separated by 350 nucleotides Synpcc7942_0490 and Synpcc7942_0491 are separated by 259 nucleotides Synpcc7942_0491 and ribF are separated by 39 nucleotides
Synpcc7942_0489: aldA - aldehyde dehydrogenase, at 475,665 to 477,044
aldA
Synpcc7942_0490: Synpcc7942_0490 - diguanylate cyclase with PAS/PAC sensor, at 477,395 to 479,440
_0490
Synpcc7942_0491: Synpcc7942_0491 - hypothetical protein, at 479,700 to 479,837
_0491
Synpcc7942_0492: ribF - bifunctional riboflavin kinase/FMN adenylyltransferase, at 479,877 to 480,824
ribF
Position (kb) 477
478
479
480 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 476.399 kb on + strand, within aldA at 476.421 kb on + strand, within aldA at 476.478 kb on + strand, within aldA at 476.495 kb on + strand, within aldA at 476.498 kb on - strand, within aldA at 476.507 kb on - strand, within aldA at 476.541 kb on + strand, within aldA at 476.601 kb on + strand, within aldA at 476.614 kb on + strand, within aldA at 476.622 kb on - strand, within aldA at 476.637 kb on - strand, within aldA at 476.670 kb on - strand, within aldA at 476.672 kb on + strand, within aldA at 476.680 kb on + strand, within aldA at 476.685 kb on + strand, within aldA at 476.688 kb on - strand, within aldA at 476.692 kb on + strand, within aldA at 476.701 kb on - strand, within aldA at 476.710 kb on + strand, within aldA at 476.718 kb on - strand, within aldA at 476.728 kb on - strand, within aldA at 476.728 kb on - strand, within aldA at 476.730 kb on + strand, within aldA at 476.811 kb on - strand, within aldA at 476.833 kb on + strand, within aldA at 476.841 kb on - strand, within aldA at 476.847 kb on + strand, within aldA at 476.855 kb on - strand, within aldA at 476.874 kb on - strand, within aldA at 476.876 kb on + strand, within aldA at 476.876 kb on + strand, within aldA at 476.876 kb on + strand, within aldA at 476.897 kb on - strand, within aldA at 476.935 kb on + strand at 476.951 kb on + strand at 476.959 kb on - strand at 476.989 kb on - strand at 477.040 kb on - strand at 477.047 kb on - strand at 477.050 kb on - strand at 477.208 kb on + strand at 477.236 kb on + strand at 477.255 kb on + strand at 477.256 kb on - strand at 477.260 kb on + strand at 477.263 kb on - strand at 477.263 kb on - strand at 477.278 kb on - strand at 477.278 kb on - strand at 477.302 kb on + strand at 477.529 kb on + strand at 477.844 kb on - strand, within Synpcc7942_0490 at 477.882 kb on + strand, within Synpcc7942_0490 at 477.915 kb on + strand, within Synpcc7942_0490 at 478.057 kb on + strand, within Synpcc7942_0490 at 478.057 kb on - strand, within Synpcc7942_0490 at 478.065 kb on - strand, within Synpcc7942_0490 at 478.072 kb on + strand, within Synpcc7942_0490 at 478.080 kb on - strand, within Synpcc7942_0490 at 478.161 kb on - strand, within Synpcc7942_0490 at 478.219 kb on + strand, within Synpcc7942_0490 at 478.249 kb on + strand, within Synpcc7942_0490 at 478.303 kb on - strand, within Synpcc7942_0490 at 478.310 kb on - strand, within Synpcc7942_0490 at 478.360 kb on + strand, within Synpcc7942_0490 at 478.477 kb on + strand, within Synpcc7942_0490 at 478.477 kb on + strand, within Synpcc7942_0490 at 478.479 kb on + strand, within Synpcc7942_0490 at 478.485 kb on - strand, within Synpcc7942_0490 at 478.487 kb on - strand, within Synpcc7942_0490 at 478.507 kb on + strand, within Synpcc7942_0490 at 478.517 kb on + strand, within Synpcc7942_0490 at 478.525 kb on - strand, within Synpcc7942_0490 at 478.541 kb on - strand, within Synpcc7942_0490 at 478.564 kb on + strand, within Synpcc7942_0490 at 478.757 kb on - strand, within Synpcc7942_0490 at 478.769 kb on + strand, within Synpcc7942_0490 at 479.032 kb on - strand, within Synpcc7942_0490 at 479.116 kb on + strand, within Synpcc7942_0490 at 479.116 kb on + strand, within Synpcc7942_0490 at 479.123 kb on - strand, within Synpcc7942_0490 at 479.125 kb on + strand, within Synpcc7942_0490 at 479.185 kb on + strand, within Synpcc7942_0490 at 479.185 kb on + strand, within Synpcc7942_0490 at 479.185 kb on + strand, within Synpcc7942_0490 at 479.193 kb on - strand, within Synpcc7942_0490 at 479.220 kb on + strand, within Synpcc7942_0490 at 479.220 kb on + strand, within Synpcc7942_0490 at 479.271 kb on + strand at 479.312 kb on - strand at 479.346 kb on - strand at 479.392 kb on + strand at 479.473 kb on + strand at 479.473 kb on + strand at 479.620 kb on - strand at 479.655 kb on - strand at 479.658 kb on + strand at 479.666 kb on - strand at 479.737 kb on - strand, within Synpcc7942_0491 at 479.737 kb on - strand, within Synpcc7942_0491 at 479.737 kb on - strand, within Synpcc7942_0491 at 479.737 kb on - strand, within Synpcc7942_0491 at 479.762 kb on + strand, within Synpcc7942_0491 at 479.770 kb on - strand, within Synpcc7942_0491 at 479.781 kb on - strand, within Synpcc7942_0491 at 479.788 kb on + strand, within Synpcc7942_0491 at 479.804 kb on + strand, within Synpcc7942_0491 at 479.811 kb on + strand, within Synpcc7942_0491 at 479.820 kb on - strand, within Synpcc7942_0491 at 479.832 kb on - strand at 479.849 kb on - strand at 479.899 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Time0 for outgrown library 2.0 remove 476,399 + aldA Synpcc7942_0489 0.53 +0.0 476,421 + aldA Synpcc7942_0489 0.55 +0.0 476,478 + aldA Synpcc7942_0489 0.59 -2.3 476,495 + aldA Synpcc7942_0489 0.60 -0.3 476,498 - aldA Synpcc7942_0489 0.60 +0.2 476,507 - aldA Synpcc7942_0489 0.61 +1.2 476,541 + aldA Synpcc7942_0489 0.63 -0.5 476,601 + aldA Synpcc7942_0489 0.68 -0.3 476,614 + aldA Synpcc7942_0489 0.69 -1.1 476,622 - aldA Synpcc7942_0489 0.69 +0.3 476,637 - aldA Synpcc7942_0489 0.70 -0.1 476,670 - aldA Synpcc7942_0489 0.73 +0.9 476,672 + aldA Synpcc7942_0489 0.73 +0.2 476,680 + aldA Synpcc7942_0489 0.74 -1.7 476,685 + aldA Synpcc7942_0489 0.74 -0.1 476,688 - aldA Synpcc7942_0489 0.74 -2.4 476,692 + aldA Synpcc7942_0489 0.74 -1.5 476,701 - aldA Synpcc7942_0489 0.75 +0.9 476,710 + aldA Synpcc7942_0489 0.76 -0.0 476,718 - aldA Synpcc7942_0489 0.76 +1.2 476,728 - aldA Synpcc7942_0489 0.77 +0.0 476,728 - aldA Synpcc7942_0489 0.77 -0.4 476,730 + aldA Synpcc7942_0489 0.77 -0.3 476,811 - aldA Synpcc7942_0489 0.83 +0.2 476,833 + aldA Synpcc7942_0489 0.85 +0.0 476,841 - aldA Synpcc7942_0489 0.85 -0.1 476,847 + aldA Synpcc7942_0489 0.86 +0.1 476,855 - aldA Synpcc7942_0489 0.86 -0.1 476,874 - aldA Synpcc7942_0489 0.88 -2.7 476,876 + aldA Synpcc7942_0489 0.88 +0.9 476,876 + aldA Synpcc7942_0489 0.88 -3.0 476,876 + aldA Synpcc7942_0489 0.88 -1.3 476,897 - aldA Synpcc7942_0489 0.89 -1.1 476,935 + -2.0 476,951 + -1.6 476,959 - -0.7 476,989 - -1.6 477,040 - -0.8 477,047 - -0.7 477,050 - -0.8 477,208 + -0.3 477,236 + -0.2 477,255 + -1.3 477,256 - -2.7 477,260 + -0.9 477,263 - -1.5 477,263 - -0.4 477,278 - -0.7 477,278 - -0.2 477,302 + -0.1 477,529 + -4.3 477,844 - Synpcc7942_0490 0.22 -0.6 477,882 + Synpcc7942_0490 0.24 -0.5 477,915 + Synpcc7942_0490 0.25 -1.1 478,057 + Synpcc7942_0490 0.32 +1.5 478,057 - Synpcc7942_0490 0.32 -2.7 478,065 - Synpcc7942_0490 0.33 -0.2 478,072 + Synpcc7942_0490 0.33 +2.1 478,080 - Synpcc7942_0490 0.33 -0.4 478,161 - Synpcc7942_0490 0.37 -0.7 478,219 + Synpcc7942_0490 0.40 +0.6 478,249 + Synpcc7942_0490 0.42 -0.8 478,303 - Synpcc7942_0490 0.44 +0.5 478,310 - Synpcc7942_0490 0.45 -1.1 478,360 + Synpcc7942_0490 0.47 +0.4 478,477 + Synpcc7942_0490 0.53 -2.3 478,477 + Synpcc7942_0490 0.53 +0.1 478,479 + Synpcc7942_0490 0.53 +1.2 478,485 - Synpcc7942_0490 0.53 -0.7 478,487 - Synpcc7942_0490 0.53 -1.0 478,507 + Synpcc7942_0490 0.54 -2.5 478,517 + Synpcc7942_0490 0.55 -2.6 478,525 - Synpcc7942_0490 0.55 -3.0 478,541 - Synpcc7942_0490 0.56 -0.5 478,564 + Synpcc7942_0490 0.57 -1.3 478,757 - Synpcc7942_0490 0.67 -1.1 478,769 + Synpcc7942_0490 0.67 +1.0 479,032 - Synpcc7942_0490 0.80 -2.6 479,116 + Synpcc7942_0490 0.84 +1.5 479,116 + Synpcc7942_0490 0.84 +1.0 479,123 - Synpcc7942_0490 0.84 -2.4 479,125 + Synpcc7942_0490 0.85 -0.7 479,185 + Synpcc7942_0490 0.87 -0.1 479,185 + Synpcc7942_0490 0.87 -0.9 479,185 + Synpcc7942_0490 0.87 -2.0 479,193 - Synpcc7942_0490 0.88 -0.1 479,220 + Synpcc7942_0490 0.89 +1.4 479,220 + Synpcc7942_0490 0.89 +0.5 479,271 + -0.9 479,312 - +0.9 479,346 - -0.3 479,392 + -3.0 479,473 + -1.9 479,473 + -3.9 479,620 - -2.3 479,655 - +0.2 479,658 + +2.1 479,666 - +0.7 479,737 - Synpcc7942_0491 0.27 +2.8 479,737 - Synpcc7942_0491 0.27 +1.1 479,737 - Synpcc7942_0491 0.27 +0.7 479,737 - Synpcc7942_0491 0.27 -2.1 479,762 + Synpcc7942_0491 0.45 -2.2 479,770 - Synpcc7942_0491 0.51 -0.2 479,781 - Synpcc7942_0491 0.59 -2.8 479,788 + Synpcc7942_0491 0.64 +0.4 479,804 + Synpcc7942_0491 0.75 +1.0 479,811 + Synpcc7942_0491 0.80 -0.1 479,820 - Synpcc7942_0491 0.87 +0.1 479,832 - +1.3 479,849 - +1.5 479,899 + -0.0
Or see this region's nucleotide sequence