Experiment: Time0 for outgrown library 2.0
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt birA and Synpcc7942_0346 are separated by 45 nucleotides Synpcc7942_0346 and Synpcc7942_0347 are separated by 156 nucleotides Synpcc7942_0347 and recA are separated by 245 nucleotides
Synpcc7942_0345: birA - Biotin--acetyl-CoA-carboxylase ligase, at 338,536 to 339,306
birA
Synpcc7942_0346: Synpcc7942_0346 - Protein of unknown function DUF152, at 339,352 to 340,131
_0346
Synpcc7942_0347: Synpcc7942_0347 - hypothetical protein, at 340,288 to 340,452
_0347
Synpcc7942_0348: recA - recombinase A, at 340,698 to 341,768
recA
Position (kb) 340
341 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 339.336 kb on + strand at 339.344 kb on + strand at 339.371 kb on - strand at 339.532 kb on - strand, within Synpcc7942_0346 at 339.544 kb on + strand, within Synpcc7942_0346 at 339.600 kb on + strand, within Synpcc7942_0346 at 339.628 kb on + strand, within Synpcc7942_0346 at 339.653 kb on + strand, within Synpcc7942_0346 at 339.686 kb on + strand, within Synpcc7942_0346 at 339.689 kb on - strand, within Synpcc7942_0346 at 339.830 kb on - strand, within Synpcc7942_0346 at 339.830 kb on - strand, within Synpcc7942_0346 at 339.847 kb on + strand, within Synpcc7942_0346 at 339.937 kb on + strand, within Synpcc7942_0346 at 340.034 kb on - strand, within Synpcc7942_0346 at 340.095 kb on + strand at 340.140 kb on + strand at 340.196 kb on - strand at 340.222 kb on + strand at 340.222 kb on + strand at 340.222 kb on + strand at 340.222 kb on + strand at 340.222 kb on + strand at 340.230 kb on - strand at 340.230 kb on - strand at 340.230 kb on - strand at 340.344 kb on - strand, within Synpcc7942_0347 at 340.373 kb on + strand, within Synpcc7942_0347 at 340.373 kb on + strand, within Synpcc7942_0347 at 340.373 kb on + strand, within Synpcc7942_0347 at 340.381 kb on - strand, within Synpcc7942_0347 at 340.429 kb on + strand, within Synpcc7942_0347 at 340.447 kb on + strand at 340.492 kb on - strand at 340.492 kb on - strand at 340.531 kb on + strand at 340.531 kb on + strand at 340.539 kb on + strand at 340.539 kb on + strand at 340.539 kb on - strand at 340.578 kb on + strand at 340.589 kb on - strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.591 kb on + strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.599 kb on - strand at 340.603 kb on + strand at 340.606 kb on - strand at 340.610 kb on + strand at 340.610 kb on + strand at 340.610 kb on + strand at 340.611 kb on - strand at 340.618 kb on - strand at 340.696 kb on + strand at 340.720 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Time0 for outgrown library 2.0 remove 339,336 + +0.9 339,344 + -0.2 339,371 - -1.7 339,532 - Synpcc7942_0346 0.23 +3.1 339,544 + Synpcc7942_0346 0.25 +3.1 339,600 + Synpcc7942_0346 0.32 +2.6 339,628 + Synpcc7942_0346 0.35 +3.1 339,653 + Synpcc7942_0346 0.39 +3.1 339,686 + Synpcc7942_0346 0.43 +2.1 339,689 - Synpcc7942_0346 0.43 +0.6 339,830 - Synpcc7942_0346 0.61 +2.1 339,830 - Synpcc7942_0346 0.61 +3.1 339,847 + Synpcc7942_0346 0.63 +2.1 339,937 + Synpcc7942_0346 0.75 +3.1 340,034 - Synpcc7942_0346 0.87 -0.2 340,095 + +3.1 340,140 + +0.9 340,196 - +1.1 340,222 + -1.8 340,222 + +0.6 340,222 + +1.4 340,222 + -0.1 340,222 + +0.5 340,230 - -0.0 340,230 - -0.5 340,230 - +0.8 340,344 - Synpcc7942_0347 0.34 +0.1 340,373 + Synpcc7942_0347 0.52 -1.7 340,373 + Synpcc7942_0347 0.52 -2.4 340,373 + Synpcc7942_0347 0.52 -0.0 340,381 - Synpcc7942_0347 0.56 -0.0 340,429 + Synpcc7942_0347 0.85 +0.0 340,447 + +2.1 340,492 - -2.3 340,492 - -3.0 340,531 + +1.3 340,531 + -1.0 340,539 + -0.3 340,539 + +0.7 340,539 - +0.8 340,578 + -0.8 340,589 - +0.3 340,591 + -1.1 340,591 + -1.7 340,591 + -2.0 340,591 + +0.5 340,591 + -0.8 340,591 + -0.9 340,591 + -0.2 340,591 + +0.3 340,591 + -0.6 340,591 + -1.0 340,591 + -1.6 340,591 + -0.2 340,591 + -0.7 340,591 + -1.0 340,591 + -0.7 340,591 + -1.9 340,591 + +0.8 340,591 + +1.0 340,599 - +1.4 340,599 - +0.3 340,599 - -0.6 340,599 - +0.1 340,599 - -1.0 340,599 - -1.1 340,599 - -0.9 340,599 - -0.6 340,599 - +0.8 340,599 - -2.0 340,599 - -1.3 340,599 - +0.9 340,599 - -1.8 340,599 - -1.6 340,599 - +0.5 340,603 + +0.5 340,606 - -1.9 340,610 + +1.4 340,610 + -1.6 340,610 + +0.5 340,611 - -1.3 340,618 - +0.1 340,696 + -1.3 340,720 - -1.1
Or see this region's nucleotide sequence