Experiment: R2A with (5z)-4-bromo-5-(bromomethylene)-2(5h)-furanone 10 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_003564 and ABIE53_003565 are separated by 6 nucleotides ABIE53_003565 and ABIE53_003566 are separated by 194 nucleotides ABIE53_003566 and ABIE53_003567 are separated by 71 nucleotides ABIE53_003567 and ABIE53_003568 are separated by 164 nucleotides
ABIE53_003564: ABIE53_003564 - hypothetical protein, at 4,005,733 to 4,006,386
_003564
ABIE53_003565: ABIE53_003565 - thiol-disulfide isomerase/thioredoxin, at 4,006,393 to 4,006,965
_003565
ABIE53_003566: ABIE53_003566 - 3-dehydroquinate dehydratase-2, at 4,007,160 to 4,007,615
_003566
ABIE53_003567: ABIE53_003567 - acetyl-CoA carboxylase biotin carboxyl carrier protein, at 4,007,687 to 4,008,166
_003567
ABIE53_003568: ABIE53_003568 - acetyl-CoA carboxylase biotin carboxylase subunit, at 4,008,331 to 4,009,698
_003568
Position (kb)
4007
4008 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4006.183 kb on + strand, within ABIE53_003564 at 4006.184 kb on - strand, within ABIE53_003564 at 4006.184 kb on - strand, within ABIE53_003564 at 4006.264 kb on + strand, within ABIE53_003564 at 4006.264 kb on + strand, within ABIE53_003564 at 4006.264 kb on + strand, within ABIE53_003564 at 4006.265 kb on - strand, within ABIE53_003564 at 4006.265 kb on - strand, within ABIE53_003564 at 4006.384 kb on + strand at 4006.384 kb on + strand at 4006.384 kb on + strand at 4006.384 kb on + strand at 4006.384 kb on + strand at 4006.385 kb on - strand at 4006.385 kb on - strand at 4006.385 kb on - strand at 4006.385 kb on - strand at 4006.385 kb on - strand at 4006.385 kb on - strand at 4006.385 kb on - strand at 4006.385 kb on - strand at 4006.608 kb on - strand, within ABIE53_003565 at 4006.795 kb on + strand, within ABIE53_003565 at 4006.804 kb on + strand, within ABIE53_003565 at 4006.804 kb on + strand, within ABIE53_003565 at 4006.804 kb on + strand, within ABIE53_003565 at 4006.805 kb on - strand, within ABIE53_003565 at 4006.805 kb on - strand, within ABIE53_003565 at 4006.851 kb on + strand, within ABIE53_003565 at 4006.877 kb on + strand, within ABIE53_003565 at 4006.877 kb on + strand, within ABIE53_003565 at 4006.877 kb on + strand, within ABIE53_003565 at 4006.877 kb on + strand, within ABIE53_003565 at 4006.916 kb on + strand at 4007.100 kb on + strand at 4007.226 kb on + strand, within ABIE53_003566 at 4007.226 kb on + strand, within ABIE53_003566 at 4007.394 kb on + strand, within ABIE53_003566 at 4007.441 kb on + strand, within ABIE53_003566 at 4007.505 kb on + strand, within ABIE53_003566 at 4007.505 kb on + strand, within ABIE53_003566 at 4007.556 kb on + strand, within ABIE53_003566 at 4007.556 kb on + strand, within ABIE53_003566 at 4007.556 kb on + strand, within ABIE53_003566 at 4007.556 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.560 kb on + strand, within ABIE53_003566 at 4007.562 kb on + strand, within ABIE53_003566 at 4007.562 kb on + strand, within ABIE53_003566 at 4007.562 kb on + strand, within ABIE53_003566 at 4007.562 kb on + strand, within ABIE53_003566 at 4007.630 kb on + strand at 4007.630 kb on + strand at 4007.630 kb on + strand at 4008.164 kb on + strand at 4008.164 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with (5z)-4-bromo-5-(bromomethylene)-2(5h)-furanone 10 uM remove 4,006,183 + ABIE53_003564 0.69 +1.3 4,006,184 - ABIE53_003564 0.69 -3.7 4,006,184 - ABIE53_003564 0.69 +1.3 4,006,264 + ABIE53_003564 0.81 -0.7 4,006,264 + ABIE53_003564 0.81 -1.7 4,006,264 + ABIE53_003564 0.81 -0.8 4,006,265 - ABIE53_003564 0.81 +0.3 4,006,265 - ABIE53_003564 0.81 +0.3 4,006,384 + -0.9 4,006,384 + +0.4 4,006,384 + -0.5 4,006,384 + -1.8 4,006,384 + -2.0 4,006,385 - -2.1 4,006,385 - -0.2 4,006,385 - -1.0 4,006,385 - -1.2 4,006,385 - -1.6 4,006,385 - -1.3 4,006,385 - -2.8 4,006,385 - -1.0 4,006,608 - ABIE53_003565 0.38 +0.4 4,006,795 + ABIE53_003565 0.70 -1.2 4,006,804 + ABIE53_003565 0.72 -1.6 4,006,804 + ABIE53_003565 0.72 -1.0 4,006,804 + ABIE53_003565 0.72 +0.4 4,006,805 - ABIE53_003565 0.72 -0.2 4,006,805 - ABIE53_003565 0.72 -3.0 4,006,851 + ABIE53_003565 0.80 +0.4 4,006,877 + ABIE53_003565 0.84 -2.9 4,006,877 + ABIE53_003565 0.84 -0.6 4,006,877 + ABIE53_003565 0.84 -2.9 4,006,877 + ABIE53_003565 0.84 -0.5 4,006,916 + -1.6 4,007,100 + -1.0 4,007,226 + ABIE53_003566 0.14 -1.0 4,007,226 + ABIE53_003566 0.14 -0.2 4,007,394 + ABIE53_003566 0.51 +2.2 4,007,441 + ABIE53_003566 0.62 +0.4 4,007,505 + ABIE53_003566 0.76 -2.6 4,007,505 + ABIE53_003566 0.76 +0.4 4,007,556 + ABIE53_003566 0.87 -1.5 4,007,556 + ABIE53_003566 0.87 +0.4 4,007,556 + ABIE53_003566 0.87 -2.5 4,007,556 + ABIE53_003566 0.87 -1.8 4,007,560 + ABIE53_003566 0.88 +2.9 4,007,560 + ABIE53_003566 0.88 +0.4 4,007,560 + ABIE53_003566 0.88 +0.4 4,007,560 + ABIE53_003566 0.88 -1.6 4,007,560 + ABIE53_003566 0.88 +3.4 4,007,560 + ABIE53_003566 0.88 +2.4 4,007,560 + ABIE53_003566 0.88 -1.0 4,007,560 + ABIE53_003566 0.88 +1.4 4,007,560 + ABIE53_003566 0.88 -2.1 4,007,560 + ABIE53_003566 0.88 -1.6 4,007,560 + ABIE53_003566 0.88 -2.0 4,007,560 + ABIE53_003566 0.88 -4.6 4,007,560 + ABIE53_003566 0.88 -0.2 4,007,560 + ABIE53_003566 0.88 +0.4 4,007,560 + ABIE53_003566 0.88 -2.7 4,007,560 + ABIE53_003566 0.88 -1.0 4,007,560 + ABIE53_003566 0.88 -0.2 4,007,560 + ABIE53_003566 0.88 -1.0 4,007,560 + ABIE53_003566 0.88 -0.1 4,007,562 + ABIE53_003566 0.88 +0.8 4,007,562 + ABIE53_003566 0.88 -0.8 4,007,562 + ABIE53_003566 0.88 -0.6 4,007,562 + ABIE53_003566 0.88 -1.1 4,007,630 + -1.6 4,007,630 + +2.4 4,007,630 + +1.4 4,008,164 + -2.2 4,008,164 + +0.8
Or see this region's nucleotide sequence