Experiment: MoYLS4 media with N2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2651 and DvMF_2652 are separated by 157 nucleotides DvMF_2652 and DvMF_2653 are separated by 61 nucleotides DvMF_2653 and DvMF_2654 are separated by 171 nucleotides DvMF_2654 and DvMF_2655 are separated by 213 nucleotides
DvMF_2651: DvMF_2651 - dihydrouridine synthase DuS (RefSeq), at 3,352,099 to 3,353,250
_2651
DvMF_2652: DvMF_2652 - transcriptional regulator, MerR family (RefSeq), at 3,353,408 to 3,353,779
_2652
DvMF_2653: DvMF_2653 - Carboxymuconolactone decarboxylase (RefSeq), at 3,353,841 to 3,354,257
_2653
DvMF_2654: DvMF_2654 - acetyltransferase (RefSeq), at 3,354,429 to 3,354,938
_2654
DvMF_2655: DvMF_2655 - hypothetical protein (RefSeq), at 3,355,152 to 3,355,688
_2655
Position (kb) 3353
3354
3355 Strain fitness (log2 ratio) -1
0
1 at 3352.857 kb on - strand, within DvMF_2651 at 3353.385 kb on - strand at 3353.433 kb on + strand at 3353.433 kb on + strand at 3353.448 kb on + strand, within DvMF_2652 at 3353.792 kb on + strand at 3353.833 kb on + strand at 3354.183 kb on - strand, within DvMF_2653 at 3354.384 kb on - strand at 3355.080 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 media with N2 remove 3,352,857 - DvMF_2651 0.66 +0.1 3,353,385 - -1.2 3,353,433 + +0.1 3,353,433 + -0.4 3,353,448 + DvMF_2652 0.11 -0.7 3,353,792 + -0.2 3,353,833 + -0.9 3,354,183 - DvMF_2653 0.82 +0.5 3,354,384 - +0.0 3,355,080 + -0.9
Or see this region's nucleotide sequence