Experiment: MoYLS4 media with N2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0048 and DvMF_0049 are separated by 264 nucleotides DvMF_0049 and DvMF_0050 are separated by 278 nucleotides DvMF_0050 and DvMF_0051 are separated by 226 nucleotides
DvMF_0048: DvMF_0048 - argininosuccinate lyase (RefSeq), at 63,998 to 65,380
_0048
DvMF_0049: DvMF_0049 - hypothetical protein (RefSeq), at 65,645 to 66,274
_0049
DvMF_0050: DvMF_0050 - RNP-1 like RNA-binding protein (RefSeq), at 66,553 to 66,819
_0050
DvMF_0051: DvMF_0051 - extracellular solute-binding protein family 3 (RefSeq), at 67,046 to 67,840
_0051
Position (kb) 65
66
67 Strain fitness (log2 ratio) -1
0
1 at 64.646 kb on + strand, within DvMF_0048 at 64.852 kb on + strand, within DvMF_0048 at 64.972 kb on + strand, within DvMF_0048 at 65.213 kb on + strand, within DvMF_0048 at 65.331 kb on + strand at 65.507 kb on - strand at 65.667 kb on + strand at 65.700 kb on - strand at 65.818 kb on + strand, within DvMF_0049 at 65.904 kb on + strand, within DvMF_0049 at 66.116 kb on + strand, within DvMF_0049 at 66.136 kb on + strand, within DvMF_0049 at 66.136 kb on + strand, within DvMF_0049 at 66.271 kb on - strand at 66.439 kb on + strand at 66.500 kb on - strand at 67.057 kb on + strand at 67.072 kb on + strand at 67.211 kb on - strand, within DvMF_0051 at 67.211 kb on - strand, within DvMF_0051
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 media with N2 remove 64,646 + DvMF_0048 0.47 -1.1 64,852 + DvMF_0048 0.62 -1.4 64,972 + DvMF_0048 0.70 -1.1 65,213 + DvMF_0048 0.88 -1.0 65,331 + -1.4 65,507 - -0.7 65,667 + -0.0 65,700 - -0.1 65,818 + DvMF_0049 0.27 -1.6 65,904 + DvMF_0049 0.41 -0.3 66,116 + DvMF_0049 0.75 -0.7 66,136 + DvMF_0049 0.78 -0.4 66,136 + DvMF_0049 0.78 +0.0 66,271 - -0.4 66,439 + -0.5 66,500 - -1.0 67,057 + -0.3 67,072 + -0.4 67,211 - DvMF_0051 0.21 -0.2 67,211 - DvMF_0051 0.21 -0.1
Or see this region's nucleotide sequence