Experiment: Pyruvate-Sulfite (60-20mM) with Tungstate 2mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hmcF and hmcE are separated by 23 nucleotides hmcE and hmcD are separated by 21 nucleotides hmcD and hmcC are separated by 15 nucleotides hmcC and hmcB are separated by 0 nucleotides
DVU0531: hmcF - HmcF, 52.7 kd protein in hmc operon (voordouw), at 602,603 to 603,988
hmcF
DVU0532: hmcE - HmcE, 25.3 kd protein in hmc operon (voordouw), at 604,012 to 604,692
hmcE
DVU0533: hmcD - HmcD, 5.8 kd protein in hmc operon (voordouw), at 604,714 to 604,857
hmcD
DVU0534: hmcC - HmcC, 43.2 kd protein in hmc operon (voordouw), at 604,873 to 606,039
hmcC
DVU0535: hmcB - 40.1 kd protein in hmc operon (HmcB) (voordouw), at 606,040 to 607,152
hmcB
Position (kb) 604
605
606
607 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 603.894 kb on + strand at 603.928 kb on - strand at 603.930 kb on + strand at 603.930 kb on + strand at 603.938 kb on - strand at 604.026 kb on - strand at 604.058 kb on + strand at 604.141 kb on - strand, within hmcE at 604.149 kb on - strand, within hmcE at 604.151 kb on - strand, within hmcE at 604.282 kb on + strand, within hmcE at 604.327 kb on + strand, within hmcE at 604.363 kb on - strand, within hmcE at 604.363 kb on - strand, within hmcE at 604.499 kb on + strand, within hmcE at 604.632 kb on - strand at 604.694 kb on + strand at 604.694 kb on + strand at 604.774 kb on - strand, within hmcD at 604.779 kb on + strand, within hmcD at 604.787 kb on - strand, within hmcD at 604.846 kb on - strand at 604.868 kb on + strand at 604.883 kb on + strand at 604.988 kb on - strand at 605.020 kb on - strand, within hmcC at 605.054 kb on - strand, within hmcC at 605.154 kb on - strand, within hmcC at 605.235 kb on - strand, within hmcC at 605.320 kb on - strand, within hmcC at 605.445 kb on - strand, within hmcC at 605.449 kb on + strand, within hmcC at 605.470 kb on + strand, within hmcC at 605.476 kb on - strand, within hmcC at 605.497 kb on + strand, within hmcC at 605.497 kb on + strand, within hmcC at 605.497 kb on + strand, within hmcC at 605.504 kb on + strand, within hmcC at 605.512 kb on - strand, within hmcC at 605.545 kb on - strand, within hmcC at 605.666 kb on + strand, within hmcC at 605.704 kb on - strand, within hmcC at 605.711 kb on + strand, within hmcC at 605.856 kb on + strand, within hmcC at 605.869 kb on - strand, within hmcC at 605.869 kb on - strand, within hmcC at 605.922 kb on - strand, within hmcC at 605.958 kb on + strand at 605.966 kb on - strand at 605.967 kb on - strand at 606.093 kb on + strand at 606.121 kb on + strand at 606.143 kb on + strand at 606.203 kb on + strand, within hmcB at 606.203 kb on + strand, within hmcB at 606.203 kb on + strand, within hmcB at 606.203 kb on + strand, within hmcB at 606.208 kb on - strand, within hmcB at 606.257 kb on + strand, within hmcB at 606.262 kb on + strand, within hmcB at 606.265 kb on - strand, within hmcB at 606.344 kb on + strand, within hmcB at 606.349 kb on + strand, within hmcB at 606.376 kb on + strand, within hmcB at 606.438 kb on - strand, within hmcB at 606.525 kb on + strand, within hmcB at 606.531 kb on - strand, within hmcB at 606.555 kb on + strand, within hmcB at 606.573 kb on + strand, within hmcB at 606.595 kb on + strand, within hmcB at 606.661 kb on + strand, within hmcB at 606.663 kb on - strand, within hmcB at 606.670 kb on - strand, within hmcB at 606.863 kb on + strand, within hmcB at 606.984 kb on + strand, within hmcB at 607.019 kb on - strand, within hmcB at 607.037 kb on - strand, within hmcB at 607.037 kb on - strand, within hmcB at 607.037 kb on - strand, within hmcB
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Sulfite (60-20mM) with Tungstate 2mM remove 603,894 + -1.2 603,928 - +1.5 603,930 + -2.0 603,930 + +0.8 603,938 - -1.1 604,026 - -2.9 604,058 + -0.0 604,141 - hmcE DVU0532 0.19 -1.4 604,149 - hmcE DVU0532 0.20 -0.1 604,151 - hmcE DVU0532 0.20 -0.7 604,282 + hmcE DVU0532 0.40 -1.1 604,327 + hmcE DVU0532 0.46 -1.8 604,363 - hmcE DVU0532 0.52 -0.5 604,363 - hmcE DVU0532 0.52 +0.7 604,499 + hmcE DVU0532 0.72 +0.2 604,632 - -1.9 604,694 + -1.6 604,694 + +0.1 604,774 - hmcD DVU0533 0.42 +1.1 604,779 + hmcD DVU0533 0.45 +0.8 604,787 - hmcD DVU0533 0.51 -1.5 604,846 - +0.4 604,868 + -1.0 604,883 + -1.3 604,988 - +0.4 605,020 - hmcC DVU0534 0.13 -0.5 605,054 - hmcC DVU0534 0.16 +0.3 605,154 - hmcC DVU0534 0.24 -1.9 605,235 - hmcC DVU0534 0.31 -0.8 605,320 - hmcC DVU0534 0.38 -2.9 605,445 - hmcC DVU0534 0.49 -0.3 605,449 + hmcC DVU0534 0.49 -1.1 605,470 + hmcC DVU0534 0.51 -0.5 605,476 - hmcC DVU0534 0.52 -1.8 605,497 + hmcC DVU0534 0.53 -1.2 605,497 + hmcC DVU0534 0.53 +0.1 605,497 + hmcC DVU0534 0.53 +0.7 605,504 + hmcC DVU0534 0.54 -0.0 605,512 - hmcC DVU0534 0.55 +0.0 605,545 - hmcC DVU0534 0.58 -0.9 605,666 + hmcC DVU0534 0.68 +0.6 605,704 - hmcC DVU0534 0.71 +0.5 605,711 + hmcC DVU0534 0.72 -0.1 605,856 + hmcC DVU0534 0.84 -0.4 605,869 - hmcC DVU0534 0.85 -1.1 605,869 - hmcC DVU0534 0.85 -1.4 605,922 - hmcC DVU0534 0.90 +0.3 605,958 + +0.6 605,966 - -1.2 605,967 - -1.2 606,093 + -1.0 606,121 + -0.7 606,143 + -1.1 606,203 + hmcB DVU0535 0.15 -2.8 606,203 + hmcB DVU0535 0.15 +0.8 606,203 + hmcB DVU0535 0.15 -0.9 606,203 + hmcB DVU0535 0.15 +3.1 606,208 - hmcB DVU0535 0.15 -0.9 606,257 + hmcB DVU0535 0.19 -0.3 606,262 + hmcB DVU0535 0.20 -1.4 606,265 - hmcB DVU0535 0.20 +0.3 606,344 + hmcB DVU0535 0.27 -1.6 606,349 + hmcB DVU0535 0.28 +0.5 606,376 + hmcB DVU0535 0.30 +0.8 606,438 - hmcB DVU0535 0.36 -1.2 606,525 + hmcB DVU0535 0.44 -0.7 606,531 - hmcB DVU0535 0.44 -0.7 606,555 + hmcB DVU0535 0.46 -2.4 606,573 + hmcB DVU0535 0.48 -0.5 606,595 + hmcB DVU0535 0.50 -0.2 606,661 + hmcB DVU0535 0.56 -2.1 606,663 - hmcB DVU0535 0.56 +0.2 606,670 - hmcB DVU0535 0.57 -1.3 606,863 + hmcB DVU0535 0.74 -0.4 606,984 + hmcB DVU0535 0.85 -0.2 607,019 - hmcB DVU0535 0.88 +0.2 607,037 - hmcB DVU0535 0.90 -1.8 607,037 - hmcB DVU0535 0.90 -1.3 607,037 - hmcB DVU0535 0.90 -1.2
Or see this region's nucleotide sequence