Experiment: Pyruvate-Sulfite (60-20mM) with Tungstate 2mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0151 and ppsA are separated by 69 nucleotides ppsA and tRNA-Leu are separated by 178 nucleotides tRNA-Leu and DVU0153 are separated by 191 nucleotides
DVU0151: DVU0151 - HAMP domain/sigma-54 interaction domain protein (TIGR), at 184,043 to 186,916
DVU0151
DVU0152: ppsA - phosphoenolpyruvate synthase-related protein (TIGR), at 186,986 to 189,547
ppsA
DVU_tRNA-Leu-1: tRNA-Leu - tRNA with anticodon TAA for Leu (VIMSS-AUTO), at 189,726 to 189,812
tRNA-Leu
DVU0153: DVU0153 - hypothetical protein (TIGR), at 190,004 to 190,387
DVU0153
Position (kb) 186
187
188
189
190 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 185.991 kb on - strand, within DVU0151 at 186.119 kb on + strand, within DVU0151 at 186.121 kb on + strand, within DVU0151 at 186.141 kb on + strand, within DVU0151 at 186.141 kb on + strand, within DVU0151 at 186.141 kb on + strand, within DVU0151 at 186.149 kb on - strand, within DVU0151 at 186.176 kb on + strand, within DVU0151 at 186.271 kb on - strand, within DVU0151 at 186.296 kb on - strand, within DVU0151 at 186.384 kb on + strand, within DVU0151 at 186.384 kb on + strand, within DVU0151 at 186.384 kb on + strand, within DVU0151 at 186.389 kb on + strand, within DVU0151 at 186.389 kb on + strand, within DVU0151 at 186.389 kb on + strand, within DVU0151 at 186.394 kb on + strand, within DVU0151 at 186.397 kb on - strand, within DVU0151 at 186.397 kb on - strand, within DVU0151 at 186.403 kb on + strand, within DVU0151 at 186.419 kb on + strand, within DVU0151 at 186.608 kb on - strand, within DVU0151 at 186.754 kb on + strand at 186.795 kb on - strand at 186.843 kb on + strand at 186.886 kb on - strand at 186.945 kb on - strand at 187.039 kb on - strand at 187.039 kb on - strand at 187.107 kb on - strand at 187.107 kb on - strand at 187.107 kb on - strand at 187.107 kb on - strand at 187.136 kb on + strand at 187.136 kb on + strand at 187.172 kb on + strand at 187.204 kb on + strand at 187.277 kb on + strand, within ppsA at 187.376 kb on + strand, within ppsA at 187.582 kb on + strand, within ppsA at 187.687 kb on + strand, within ppsA at 187.693 kb on - strand, within ppsA at 187.732 kb on + strand, within ppsA at 187.735 kb on - strand, within ppsA at 187.740 kb on - strand, within ppsA at 187.740 kb on - strand, within ppsA at 187.740 kb on - strand, within ppsA at 187.742 kb on + strand, within ppsA at 187.750 kb on - strand, within ppsA at 187.787 kb on + strand, within ppsA at 187.787 kb on + strand, within ppsA at 187.873 kb on - strand, within ppsA at 187.883 kb on - strand, within ppsA at 187.934 kb on - strand, within ppsA at 187.996 kb on - strand, within ppsA at 188.024 kb on - strand, within ppsA at 188.071 kb on - strand, within ppsA at 188.071 kb on - strand, within ppsA at 188.098 kb on - strand, within ppsA at 188.111 kb on + strand, within ppsA at 188.118 kb on + strand, within ppsA at 188.118 kb on + strand, within ppsA at 188.118 kb on + strand, within ppsA at 188.196 kb on + strand, within ppsA at 188.204 kb on - strand, within ppsA at 188.225 kb on + strand, within ppsA at 188.242 kb on + strand, within ppsA at 188.242 kb on + strand, within ppsA at 188.329 kb on + strand, within ppsA at 188.337 kb on + strand, within ppsA at 188.337 kb on + strand, within ppsA at 188.337 kb on + strand, within ppsA at 188.345 kb on - strand, within ppsA at 188.350 kb on - strand, within ppsA at 188.369 kb on - strand, within ppsA at 188.373 kb on + strand, within ppsA at 188.385 kb on + strand, within ppsA at 188.393 kb on - strand, within ppsA at 188.485 kb on + strand, within ppsA at 188.486 kb on + strand, within ppsA at 188.526 kb on + strand, within ppsA at 188.615 kb on + strand, within ppsA at 188.620 kb on + strand, within ppsA at 188.662 kb on - strand, within ppsA at 188.667 kb on - strand, within ppsA at 188.770 kb on - strand, within ppsA at 188.779 kb on + strand, within ppsA at 188.806 kb on + strand, within ppsA at 188.806 kb on + strand, within ppsA at 188.806 kb on + strand, within ppsA at 188.829 kb on - strand, within ppsA at 188.937 kb on + strand, within ppsA at 188.989 kb on + strand, within ppsA at 189.012 kb on - strand, within ppsA at 189.017 kb on + strand, within ppsA at 189.031 kb on + strand, within ppsA at 189.039 kb on - strand, within ppsA at 189.048 kb on + strand, within ppsA at 189.056 kb on - strand, within ppsA at 189.225 kb on - strand, within ppsA at 189.251 kb on + strand, within ppsA at 189.328 kb on + strand at 189.344 kb on - strand at 189.379 kb on - strand at 189.392 kb on - strand at 189.394 kb on - strand at 189.462 kb on - strand at 189.668 kb on - strand at 189.669 kb on - strand at 189.842 kb on + strand at 189.842 kb on + strand at 189.884 kb on - strand at 189.889 kb on + strand at 189.897 kb on + strand at 189.931 kb on + strand at 189.938 kb on + strand at 189.946 kb on - strand at 189.946 kb on - strand at 189.951 kb on - strand at 189.956 kb on - strand at 190.000 kb on - strand at 190.062 kb on + strand, within DVU0153 at 190.091 kb on + strand, within DVU0153 at 190.091 kb on - strand, within DVU0153 at 190.093 kb on + strand, within DVU0153 at 190.098 kb on + strand, within DVU0153 at 190.123 kb on + strand, within DVU0153 at 190.129 kb on - strand, within DVU0153 at 190.158 kb on - strand, within DVU0153 at 190.172 kb on - strand, within DVU0153 at 190.176 kb on + strand, within DVU0153 at 190.176 kb on + strand, within DVU0153 at 190.181 kb on + strand, within DVU0153 at 190.184 kb on - strand, within DVU0153 at 190.189 kb on - strand, within DVU0153 at 190.189 kb on - strand, within DVU0153 at 190.189 kb on - strand, within DVU0153 at 190.235 kb on - strand, within DVU0153 at 190.247 kb on + strand, within DVU0153 at 190.255 kb on - strand, within DVU0153 at 190.256 kb on + strand, within DVU0153 at 190.270 kb on - strand, within DVU0153 at 190.270 kb on - strand, within DVU0153 at 190.290 kb on + strand, within DVU0153 at 190.306 kb on - strand, within DVU0153 at 190.330 kb on + strand, within DVU0153 at 190.373 kb on + strand at 190.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Sulfite (60-20mM) with Tungstate 2mM remove 185,991 - DVU0151 0.68 -0.9 186,119 + DVU0151 0.72 -0.9 186,121 + DVU0151 0.72 +1.2 186,141 + DVU0151 0.73 -0.1 186,141 + DVU0151 0.73 -1.1 186,141 + DVU0151 0.73 +1.2 186,149 - DVU0151 0.73 -0.7 186,176 + DVU0151 0.74 -0.2 186,271 - DVU0151 0.78 +0.5 186,296 - DVU0151 0.78 +0.8 186,384 + DVU0151 0.81 -1.0 186,384 + DVU0151 0.81 -0.5 186,384 + DVU0151 0.81 -0.2 186,389 + DVU0151 0.82 -0.7 186,389 + DVU0151 0.82 -0.1 186,389 + DVU0151 0.82 -1.4 186,394 + DVU0151 0.82 -3.2 186,397 - DVU0151 0.82 -0.4 186,397 - DVU0151 0.82 -1.5 186,403 + DVU0151 0.82 -0.6 186,419 + DVU0151 0.83 -1.0 186,608 - DVU0151 0.89 +0.1 186,754 + +0.2 186,795 - -3.1 186,843 + -0.5 186,886 - -0.9 186,945 - -0.7 187,039 - -0.6 187,039 - +2.6 187,107 - +1.0 187,107 - -0.5 187,107 - +2.6 187,107 - -0.6 187,136 + -1.5 187,136 + -0.7 187,172 + -1.6 187,204 + -1.3 187,277 + ppsA DVU0152 0.11 +0.3 187,376 + ppsA DVU0152 0.15 -0.3 187,582 + ppsA DVU0152 0.23 +0.5 187,687 + ppsA DVU0152 0.27 +2.1 187,693 - ppsA DVU0152 0.28 +0.5 187,732 + ppsA DVU0152 0.29 -2.6 187,735 - ppsA DVU0152 0.29 -1.0 187,740 - ppsA DVU0152 0.29 -1.1 187,740 - ppsA DVU0152 0.29 -0.7 187,740 - ppsA DVU0152 0.29 -0.8 187,742 + ppsA DVU0152 0.30 +0.1 187,750 - ppsA DVU0152 0.30 -0.7 187,787 + ppsA DVU0152 0.31 -0.5 187,787 + ppsA DVU0152 0.31 +1.4 187,873 - ppsA DVU0152 0.35 -0.2 187,883 - ppsA DVU0152 0.35 -1.1 187,934 - ppsA DVU0152 0.37 +2.2 187,996 - ppsA DVU0152 0.39 -1.0 188,024 - ppsA DVU0152 0.41 -2.6 188,071 - ppsA DVU0152 0.42 +1.8 188,071 - ppsA DVU0152 0.42 -1.7 188,098 - ppsA DVU0152 0.43 -0.6 188,111 + ppsA DVU0152 0.44 -1.7 188,118 + ppsA DVU0152 0.44 -1.2 188,118 + ppsA DVU0152 0.44 -0.6 188,118 + ppsA DVU0152 0.44 +0.1 188,196 + ppsA DVU0152 0.47 -1.6 188,204 - ppsA DVU0152 0.48 +0.3 188,225 + ppsA DVU0152 0.48 -0.5 188,242 + ppsA DVU0152 0.49 -1.5 188,242 + ppsA DVU0152 0.49 -0.9 188,329 + ppsA DVU0152 0.52 -0.9 188,337 + ppsA DVU0152 0.53 -0.4 188,337 + ppsA DVU0152 0.53 -1.1 188,337 + ppsA DVU0152 0.53 +0.5 188,345 - ppsA DVU0152 0.53 -0.9 188,350 - ppsA DVU0152 0.53 +0.8 188,369 - ppsA DVU0152 0.54 -0.3 188,373 + ppsA DVU0152 0.54 -0.8 188,385 + ppsA DVU0152 0.55 -0.8 188,393 - ppsA DVU0152 0.55 -0.6 188,485 + ppsA DVU0152 0.59 -0.7 188,486 + ppsA DVU0152 0.59 -1.3 188,526 + ppsA DVU0152 0.60 -1.7 188,615 + ppsA DVU0152 0.64 -0.4 188,620 + ppsA DVU0152 0.64 -0.3 188,662 - ppsA DVU0152 0.65 -1.3 188,667 - ppsA DVU0152 0.66 -0.3 188,770 - ppsA DVU0152 0.70 -0.5 188,779 + ppsA DVU0152 0.70 +0.8 188,806 + ppsA DVU0152 0.71 -0.4 188,806 + ppsA DVU0152 0.71 -0.6 188,806 + ppsA DVU0152 0.71 +1.2 188,829 - ppsA DVU0152 0.72 -1.0 188,937 + ppsA DVU0152 0.76 -0.3 188,989 + ppsA DVU0152 0.78 -1.5 189,012 - ppsA DVU0152 0.79 +0.4 189,017 + ppsA DVU0152 0.79 -0.3 189,031 + ppsA DVU0152 0.80 +0.2 189,039 - ppsA DVU0152 0.80 +2.3 189,048 + ppsA DVU0152 0.80 -1.3 189,056 - ppsA DVU0152 0.81 -1.0 189,225 - ppsA DVU0152 0.87 +1.5 189,251 + ppsA DVU0152 0.88 -0.6 189,328 + -0.9 189,344 - +1.3 189,379 - +1.4 189,392 - -2.3 189,394 - -0.6 189,462 - -0.7 189,668 - -0.8 189,669 - -1.2 189,842 + +0.3 189,842 + -0.8 189,884 - -0.7 189,889 + -0.7 189,897 + -0.3 189,931 + +2.2 189,938 + -0.9 189,946 - -1.4 189,946 - +4.0 189,951 - -0.6 189,956 - -0.1 190,000 - +0.4 190,062 + DVU0153 0.15 -1.1 190,091 + DVU0153 0.23 -0.4 190,091 - DVU0153 0.23 -0.7 190,093 + DVU0153 0.23 -0.3 190,098 + DVU0153 0.24 -1.7 190,123 + DVU0153 0.31 -0.9 190,129 - DVU0153 0.33 +0.2 190,158 - DVU0153 0.40 -2.7 190,172 - DVU0153 0.44 -0.3 190,176 + DVU0153 0.45 -0.6 190,176 + DVU0153 0.45 +2.2 190,181 + DVU0153 0.46 -1.3 190,184 - DVU0153 0.47 +1.3 190,189 - DVU0153 0.48 +0.2 190,189 - DVU0153 0.48 +1.0 190,189 - DVU0153 0.48 +1.4 190,235 - DVU0153 0.60 -0.9 190,247 + DVU0153 0.63 -0.9 190,255 - DVU0153 0.65 +0.6 190,256 + DVU0153 0.66 -0.3 190,270 - DVU0153 0.69 +0.3 190,270 - DVU0153 0.69 +0.8 190,290 + DVU0153 0.74 +1.4 190,306 - DVU0153 0.79 -1.6 190,330 + DVU0153 0.85 -1.0 190,373 + -1.1 190,501 + -2.2
Or see this region's nucleotide sequence