Experiment: Pyruvate-Sulfite (60-20mM) with Tungstate 2mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpsU and DVU1794 are separated by 116 nucleotides DVU1794 and hup-3 are separated by 10 nucleotides hup-3 and DVU1796 overlap by 4 nucleotides DVU1796 and ksgA are separated by 82 nucleotides ksgA and DVU1798 are separated by 91 nucleotides DVU1798 and fusA-2 are separated by 6 nucleotides
DVU1792: rpsU - ribosomal protein S21 (TIGR), at 1,857,405 to 1,857,608
rpsU
DVU1794: DVU1794 - hypothetical protein (TIGR), at 1,857,725 to 1,857,928
DVU1794
DVU1795: hup-3 - DNA-binding protein HU (TIGR), at 1,857,939 to 1,858,211
hup-3
DVU1796: DVU1796 - hypothetical protein (TIGR), at 1,858,208 to 1,858,330
DVU1796
DVU1797: ksgA - dimethyladenosine transferase (TIGR), at 1,858,413 to 1,859,213
ksgA
DVU1798: DVU1798 - conserved hypothetical protein (TIGR), at 1,859,305 to 1,859,820
DVU1798
DVU1799: fusA-2 - translation elongation factor G (TIGR), at 1,859,827 to 1,861,875
fusA-2
Position (kb) 1858
1859
1860 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1857.655 kb on + strand at 1857.663 kb on - strand at 1857.799 kb on + strand, within DVU1794 at 1857.824 kb on - strand, within DVU1794 at 1857.824 kb on - strand, within DVU1794 at 1857.824 kb on - strand, within DVU1794 at 1857.863 kb on - strand, within DVU1794 at 1858.109 kb on - strand, within hup-3 at 1858.248 kb on + strand, within DVU1796 at 1858.248 kb on + strand, within DVU1796 at 1858.419 kb on - strand at 1858.508 kb on - strand, within ksgA at 1858.508 kb on - strand, within ksgA at 1858.517 kb on + strand, within ksgA at 1858.541 kb on - strand, within ksgA at 1858.543 kb on + strand, within ksgA at 1858.573 kb on - strand, within ksgA at 1858.577 kb on + strand, within ksgA at 1858.582 kb on + strand, within ksgA at 1858.585 kb on - strand, within ksgA at 1858.646 kb on + strand, within ksgA at 1858.756 kb on + strand, within ksgA at 1858.825 kb on + strand, within ksgA at 1859.067 kb on + strand, within ksgA at 1859.183 kb on + strand at 1859.186 kb on - strand at 1859.196 kb on - strand at 1859.211 kb on - strand at 1859.236 kb on + strand at 1859.244 kb on + strand at 1859.439 kb on - strand, within DVU1798 at 1859.499 kb on + strand, within DVU1798 at 1859.515 kb on + strand, within DVU1798 at 1859.515 kb on + strand, within DVU1798 at 1859.534 kb on + strand, within DVU1798 at 1859.555 kb on + strand, within DVU1798 at 1859.685 kb on - strand, within DVU1798 at 1859.685 kb on - strand, within DVU1798 at 1859.788 kb on + strand at 1859.815 kb on + strand at 1859.847 kb on - strand at 1859.890 kb on + strand at 1859.890 kb on + strand at 1860.058 kb on - strand, within fusA-2 at 1860.085 kb on + strand, within fusA-2 at 1860.097 kb on + strand, within fusA-2 at 1860.121 kb on + strand, within fusA-2
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Sulfite (60-20mM) with Tungstate 2mM remove 1,857,655 + -0.8 1,857,663 - +0.7 1,857,799 + DVU1794 0.36 +1.0 1,857,824 - DVU1794 0.49 -2.2 1,857,824 - DVU1794 0.49 -3.1 1,857,824 - DVU1794 0.49 -2.6 1,857,863 - DVU1794 0.68 +1.0 1,858,109 - hup-3 DVU1795 0.62 -0.5 1,858,248 + DVU1796 0.33 +0.7 1,858,248 + DVU1796 0.33 -1.8 1,858,419 - +1.0 1,858,508 - ksgA DVU1797 0.12 -2.0 1,858,508 - ksgA DVU1797 0.12 -2.4 1,858,517 + ksgA DVU1797 0.13 -1.8 1,858,541 - ksgA DVU1797 0.16 -1.9 1,858,543 + ksgA DVU1797 0.16 -1.6 1,858,573 - ksgA DVU1797 0.20 -1.1 1,858,577 + ksgA DVU1797 0.20 +0.1 1,858,582 + ksgA DVU1797 0.21 +1.2 1,858,585 - ksgA DVU1797 0.21 +0.0 1,858,646 + ksgA DVU1797 0.29 -0.3 1,858,756 + ksgA DVU1797 0.43 +0.2 1,858,825 + ksgA DVU1797 0.51 -1.4 1,859,067 + ksgA DVU1797 0.82 -1.7 1,859,183 + -2.1 1,859,186 - -2.1 1,859,196 - -2.7 1,859,211 - +0.1 1,859,236 + -1.3 1,859,244 + -1.4 1,859,439 - DVU1798 0.26 +1.4 1,859,499 + DVU1798 0.38 -0.8 1,859,515 + DVU1798 0.41 -0.1 1,859,515 + DVU1798 0.41 +0.9 1,859,534 + DVU1798 0.44 +0.6 1,859,555 + DVU1798 0.48 +0.5 1,859,685 - DVU1798 0.74 +0.7 1,859,685 - DVU1798 0.74 +1.6 1,859,788 + +1.7 1,859,815 + -0.1 1,859,847 - +1.4 1,859,890 + -0.8 1,859,890 + +2.5 1,860,058 - fusA-2 DVU1799 0.11 -1.9 1,860,085 + fusA-2 DVU1799 0.13 -0.2 1,860,097 + fusA-2 DVU1799 0.13 -2.8 1,860,121 + fusA-2 DVU1799 0.14 +1.0
Or see this region's nucleotide sequence