Experiment: No grazer plate rep 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbB and rfbA overlap by 4 nucleotides rfbA and Synpcc7942_2102 are separated by 11 nucleotides Synpcc7942_2102 and Synpcc7942_2103 are separated by 42 nucleotides Synpcc7942_2103 and cynS are separated by 216 nucleotides
Synpcc7942_2100: rfbB - dTDP-glucose 4,6-dehydratase, at 2,181,871 to 2,182,944
rfbB
Synpcc7942_2101: rfbA - glucose-1-phosphate thymidylyltransferase, at 2,182,941 to 2,183,825
rfbA
Synpcc7942_2102: Synpcc7942_2102 - NAD-reducing hydrogenase gamma, at 2,183,837 to 2,184,076
_2102
Synpcc7942_2103: Synpcc7942_2103 - hypothetical protein, at 2,184,119 to 2,184,523
_2103
Synpcc7942_2104: cynS - cyanate hydratase, at 2,184,740 to 2,185,180
cynS
Position (kb)
2183
2184
2185 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2182.921 kb on - strand at 2182.968 kb on - strand at 2182.991 kb on + strand at 2182.991 kb on + strand at 2182.991 kb on + strand at 2182.999 kb on - strand at 2183.003 kb on + strand at 2183.005 kb on + strand at 2183.005 kb on + strand at 2183.013 kb on - strand at 2183.013 kb on - strand at 2183.046 kb on - strand, within rfbA at 2183.063 kb on + strand, within rfbA at 2183.115 kb on + strand, within rfbA at 2183.115 kb on + strand, within rfbA at 2183.115 kb on + strand, within rfbA at 2183.115 kb on - strand, within rfbA at 2183.115 kb on - strand, within rfbA at 2183.123 kb on - strand, within rfbA at 2183.123 kb on - strand, within rfbA at 2183.141 kb on + strand, within rfbA at 2183.177 kb on + strand, within rfbA at 2183.177 kb on + strand, within rfbA at 2183.207 kb on - strand, within rfbA at 2183.214 kb on - strand, within rfbA at 2183.238 kb on + strand, within rfbA at 2183.338 kb on + strand, within rfbA at 2183.383 kb on - strand, within rfbA at 2183.432 kb on + strand, within rfbA at 2183.432 kb on + strand, within rfbA at 2183.457 kb on + strand, within rfbA at 2183.466 kb on + strand, within rfbA at 2183.501 kb on + strand, within rfbA at 2183.513 kb on - strand, within rfbA at 2183.540 kb on - strand, within rfbA at 2183.549 kb on + strand, within rfbA at 2183.557 kb on - strand, within rfbA at 2183.574 kb on + strand, within rfbA at 2183.592 kb on - strand, within rfbA at 2183.609 kb on + strand, within rfbA at 2183.628 kb on + strand, within rfbA at 2183.628 kb on + strand, within rfbA at 2183.636 kb on - strand, within rfbA at 2183.636 kb on - strand, within rfbA at 2183.721 kb on + strand, within rfbA at 2183.740 kb on - strand at 2183.746 kb on + strand at 2183.754 kb on - strand at 2183.763 kb on + strand at 2183.771 kb on - strand at 2183.804 kb on - strand at 2183.813 kb on - strand at 2183.827 kb on - strand at 2183.841 kb on + strand at 2183.849 kb on + strand at 2183.849 kb on - strand at 2183.862 kb on + strand, within Synpcc7942_2102 at 2183.897 kb on + strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.924 kb on + strand, within Synpcc7942_2102 at 2183.932 kb on - strand, within Synpcc7942_2102 at 2183.946 kb on + strand, within Synpcc7942_2102 at 2183.958 kb on + strand, within Synpcc7942_2102 at 2183.962 kb on - strand, within Synpcc7942_2102 at 2183.966 kb on - strand, within Synpcc7942_2102 at 2183.996 kb on + strand, within Synpcc7942_2102 at 2184.034 kb on + strand, within Synpcc7942_2102 at 2184.035 kb on + strand, within Synpcc7942_2102 at 2184.076 kb on + strand at 2184.097 kb on - strand at 2184.141 kb on + strand at 2184.168 kb on + strand, within Synpcc7942_2103 at 2184.170 kb on - strand, within Synpcc7942_2103 at 2184.176 kb on - strand, within Synpcc7942_2103 at 2184.186 kb on - strand, within Synpcc7942_2103 at 2184.225 kb on - strand, within Synpcc7942_2103 at 2184.305 kb on - strand, within Synpcc7942_2103 at 2184.324 kb on + strand, within Synpcc7942_2103 at 2184.335 kb on + strand, within Synpcc7942_2103 at 2184.345 kb on + strand, within Synpcc7942_2103 at 2184.353 kb on + strand, within Synpcc7942_2103 at 2184.408 kb on - strand, within Synpcc7942_2103 at 2184.426 kb on + strand, within Synpcc7942_2103 at 2184.434 kb on - strand, within Synpcc7942_2103 at 2184.468 kb on - strand, within Synpcc7942_2103 at 2184.518 kb on + strand at 2184.528 kb on - strand at 2184.562 kb on + strand at 2184.576 kb on - strand at 2184.604 kb on + strand at 2184.606 kb on + strand at 2184.606 kb on + strand at 2184.607 kb on - strand at 2184.612 kb on - strand at 2184.624 kb on - strand at 2184.624 kb on - strand at 2184.631 kb on + strand at 2184.634 kb on - strand at 2184.639 kb on - strand at 2184.696 kb on + strand at 2184.764 kb on - strand at 2184.764 kb on - strand at 2184.795 kb on + strand, within cynS at 2184.893 kb on + strand, within cynS at 2184.894 kb on + strand, within cynS at 2184.894 kb on + strand, within cynS at 2184.902 kb on - strand, within cynS at 2184.962 kb on + strand, within cynS at 2184.971 kb on - strand, within cynS at 2184.986 kb on + strand, within cynS at 2185.001 kb on - strand, within cynS at 2185.010 kb on + strand, within cynS
Per-strain Table
Position Strand Gene LocusTag Fraction No grazer plate rep 2 remove 2,182,921 - -0.5 2,182,968 - -0.8 2,182,991 + +0.6 2,182,991 + +0.2 2,182,991 + -0.6 2,182,999 - +0.3 2,183,003 + -0.4 2,183,005 + +0.4 2,183,005 + -0.7 2,183,013 - -0.3 2,183,013 - +0.2 2,183,046 - rfbA Synpcc7942_2101 0.12 +1.5 2,183,063 + rfbA Synpcc7942_2101 0.14 -1.7 2,183,115 + rfbA Synpcc7942_2101 0.20 -1.0 2,183,115 + rfbA Synpcc7942_2101 0.20 +0.0 2,183,115 + rfbA Synpcc7942_2101 0.20 +0.0 2,183,115 - rfbA Synpcc7942_2101 0.20 -0.2 2,183,115 - rfbA Synpcc7942_2101 0.20 +0.9 2,183,123 - rfbA Synpcc7942_2101 0.21 +1.0 2,183,123 - rfbA Synpcc7942_2101 0.21 -0.6 2,183,141 + rfbA Synpcc7942_2101 0.23 +0.5 2,183,177 + rfbA Synpcc7942_2101 0.27 -1.7 2,183,177 + rfbA Synpcc7942_2101 0.27 -0.5 2,183,207 - rfbA Synpcc7942_2101 0.30 -1.1 2,183,214 - rfbA Synpcc7942_2101 0.31 -0.2 2,183,238 + rfbA Synpcc7942_2101 0.34 -0.8 2,183,338 + rfbA Synpcc7942_2101 0.45 +0.6 2,183,383 - rfbA Synpcc7942_2101 0.50 -1.1 2,183,432 + rfbA Synpcc7942_2101 0.55 -1.6 2,183,432 + rfbA Synpcc7942_2101 0.55 -1.6 2,183,457 + rfbA Synpcc7942_2101 0.58 +1.0 2,183,466 + rfbA Synpcc7942_2101 0.59 +2.9 2,183,501 + rfbA Synpcc7942_2101 0.63 +1.6 2,183,513 - rfbA Synpcc7942_2101 0.65 +0.1 2,183,540 - rfbA Synpcc7942_2101 0.68 -0.7 2,183,549 + rfbA Synpcc7942_2101 0.69 -0.6 2,183,557 - rfbA Synpcc7942_2101 0.70 -0.2 2,183,574 + rfbA Synpcc7942_2101 0.72 -1.2 2,183,592 - rfbA Synpcc7942_2101 0.74 -0.2 2,183,609 + rfbA Synpcc7942_2101 0.75 -1.3 2,183,628 + rfbA Synpcc7942_2101 0.78 +1.9 2,183,628 + rfbA Synpcc7942_2101 0.78 +0.2 2,183,636 - rfbA Synpcc7942_2101 0.79 -0.1 2,183,636 - rfbA Synpcc7942_2101 0.79 +3.3 2,183,721 + rfbA Synpcc7942_2101 0.88 +1.5 2,183,740 - -0.0 2,183,746 + -1.0 2,183,754 - -1.7 2,183,763 + -1.0 2,183,771 - -0.9 2,183,804 - -1.1 2,183,813 - -0.5 2,183,827 - -1.1 2,183,841 + -0.2 2,183,849 + +0.9 2,183,849 - -0.9 2,183,862 + Synpcc7942_2102 0.10 -0.0 2,183,897 + Synpcc7942_2102 0.25 +0.1 2,183,905 - Synpcc7942_2102 0.28 -0.6 2,183,905 - Synpcc7942_2102 0.28 -0.2 2,183,905 - Synpcc7942_2102 0.28 -1.6 2,183,905 - Synpcc7942_2102 0.28 -1.4 2,183,924 + Synpcc7942_2102 0.36 -0.1 2,183,932 - Synpcc7942_2102 0.40 -1.7 2,183,946 + Synpcc7942_2102 0.45 +1.7 2,183,958 + Synpcc7942_2102 0.50 +0.7 2,183,962 - Synpcc7942_2102 0.52 -0.9 2,183,966 - Synpcc7942_2102 0.54 -0.1 2,183,996 + Synpcc7942_2102 0.66 +0.2 2,184,034 + Synpcc7942_2102 0.82 -2.0 2,184,035 + Synpcc7942_2102 0.82 -0.8 2,184,076 + -1.3 2,184,097 - +1.5 2,184,141 + -0.7 2,184,168 + Synpcc7942_2103 0.12 -2.6 2,184,170 - Synpcc7942_2103 0.13 +0.2 2,184,176 - Synpcc7942_2103 0.14 +0.1 2,184,186 - Synpcc7942_2103 0.17 +0.2 2,184,225 - Synpcc7942_2103 0.26 +0.9 2,184,305 - Synpcc7942_2103 0.46 -1.3 2,184,324 + Synpcc7942_2103 0.51 -0.6 2,184,335 + Synpcc7942_2103 0.53 +0.1 2,184,345 + Synpcc7942_2103 0.56 -1.2 2,184,353 + Synpcc7942_2103 0.58 -1.3 2,184,408 - Synpcc7942_2103 0.71 +0.0 2,184,426 + Synpcc7942_2103 0.76 -1.1 2,184,434 - Synpcc7942_2103 0.78 -2.3 2,184,468 - Synpcc7942_2103 0.86 -0.1 2,184,518 + +0.4 2,184,528 - +3.6 2,184,562 + +0.2 2,184,576 - -0.1 2,184,604 + +0.1 2,184,606 + -0.2 2,184,606 + +0.3 2,184,607 - -0.9 2,184,612 - +0.6 2,184,624 - -1.2 2,184,624 - -0.3 2,184,631 + +0.5 2,184,634 - +1.1 2,184,639 - -1.2 2,184,696 + -1.6 2,184,764 - +0.1 2,184,764 - +0.4 2,184,795 + cynS Synpcc7942_2104 0.12 -0.1 2,184,893 + cynS Synpcc7942_2104 0.35 +0.7 2,184,894 + cynS Synpcc7942_2104 0.35 -0.6 2,184,894 + cynS Synpcc7942_2104 0.35 -2.0 2,184,902 - cynS Synpcc7942_2104 0.37 +0.4 2,184,962 + cynS Synpcc7942_2104 0.50 -1.2 2,184,971 - cynS Synpcc7942_2104 0.52 +0.4 2,184,986 + cynS Synpcc7942_2104 0.56 +0.3 2,185,001 - cynS Synpcc7942_2104 0.59 -0.3 2,185,010 + cynS Synpcc7942_2104 0.61 +1.0
Or see this region's nucleotide sequence