Experiment: MoYLS4 media with NH4 no edta
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0473 and DvMF_0474 are separated by 168 nucleotides DvMF_0474 and DvMF_0475 are separated by 0 nucleotides DvMF_0475 and DvMF_0476 are separated by 217 nucleotides
DvMF_0473: DvMF_0473 - hypoxanthine phosphoribosyltransferase (RefSeq), at 555,775 to 556,314
_0473
DvMF_0474: DvMF_0474 - acetyltransferase (RefSeq), at 556,483 to 556,950
_0474
DvMF_0475: DvMF_0475 - alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen (RefSeq), at 556,951 to 557,424
_0475
DvMF_0476: DvMF_0476 - vitamin B12-dependent methionine synthase without a reactivation domain (EC 2.1.1.13) <small>(from data)</small>, at 557,642 to 560,098
_0476
Position (kb) 556
557
558 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 556.066 kb on - strand, within DvMF_0473 at 556.141 kb on + strand, within DvMF_0473 at 556.201 kb on + strand, within DvMF_0473 at 556.276 kb on - strand at 556.524 kb on + strand at 556.659 kb on - strand, within DvMF_0474 at 556.737 kb on + strand, within DvMF_0474 at 556.832 kb on + strand, within DvMF_0474 at 556.882 kb on + strand, within DvMF_0474 at 557.110 kb on - strand, within DvMF_0475 at 557.145 kb on - strand, within DvMF_0475 at 557.815 kb on + strand at 558.014 kb on + strand, within DvMF_0476 at 558.189 kb on + strand, within DvMF_0476 at 558.417 kb on - strand, within DvMF_0476
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 media with NH4 no edta remove 556,066 - DvMF_0473 0.54 -0.2 556,141 + DvMF_0473 0.68 +1.1 556,201 + DvMF_0473 0.79 +0.2 556,276 - +1.5 556,524 + -2.0 556,659 - DvMF_0474 0.38 -2.2 556,737 + DvMF_0474 0.54 -4.3 556,832 + DvMF_0474 0.75 -2.9 556,882 + DvMF_0474 0.85 -4.1 557,110 - DvMF_0475 0.34 -1.5 557,145 - DvMF_0475 0.41 -1.9 557,815 + -3.4 558,014 + DvMF_0476 0.15 -2.5 558,189 + DvMF_0476 0.22 -3.5 558,417 - DvMF_0476 0.32 -2.7
Or see this region's nucleotide sequence