Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3939 and BT3940 are separated by 549 nucleotides BT3940 and BT3941 are separated by 33 nucleotides BT3941 and BT3942 are separated by 189 nucleotides BT3942 and BT3943 are separated by 78 nucleotides
BT3939: BT3939 - single-stranded-DNA-specific exonuclease recJ (NCBI ptt file), at 5,114,434 to 5,116,152
BT3939
BT3940: BT3940 - hypothetical protein (NCBI ptt file), at 5,116,702 to 5,116,908
BT3940
BT3941: BT3941 - conserved hypothetical protein (NCBI ptt file), at 5,116,942 to 5,117,868
BT3941
BT3942: BT3942 - conserved hypothetical protein (NCBI ptt file), at 5,118,058 to 5,118,495
BT3942
BT3943: BT3943 - conserved hypothetical protein, putative translation factor (NCBI ptt file), at 5,118,574 to 5,119,137
BT3943
Position (kb) 5116
5117
5118 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5116.361 kb on + strand at 5116.361 kb on + strand at 5116.419 kb on + strand at 5116.465 kb on - strand at 5116.465 kb on - strand at 5116.465 kb on - strand at 5116.473 kb on + strand at 5116.519 kb on + strand at 5116.519 kb on + strand at 5116.520 kb on - strand at 5116.520 kb on - strand at 5116.542 kb on - strand at 5116.564 kb on - strand at 5116.586 kb on + strand at 5116.587 kb on - strand at 5116.587 kb on - strand at 5116.654 kb on - strand at 5116.794 kb on - strand, within BT3940 at 5116.931 kb on - strand at 5116.940 kb on - strand at 5116.940 kb on - strand at 5116.956 kb on - strand at 5117.013 kb on + strand at 5117.066 kb on + strand, within BT3941 at 5117.103 kb on - strand, within BT3941 at 5117.103 kb on - strand, within BT3941 at 5117.148 kb on - strand, within BT3941 at 5117.196 kb on + strand, within BT3941 at 5117.199 kb on + strand, within BT3941 at 5117.200 kb on - strand, within BT3941 at 5117.200 kb on - strand, within BT3941 at 5117.201 kb on + strand, within BT3941 at 5117.202 kb on - strand, within BT3941 at 5117.202 kb on - strand, within BT3941 at 5117.211 kb on - strand, within BT3941 at 5117.325 kb on + strand, within BT3941 at 5117.501 kb on - strand, within BT3941 at 5117.545 kb on + strand, within BT3941 at 5117.545 kb on + strand, within BT3941 at 5117.546 kb on - strand, within BT3941 at 5117.575 kb on - strand, within BT3941 at 5117.601 kb on + strand, within BT3941 at 5117.638 kb on - strand, within BT3941 at 5117.818 kb on + strand at 5117.818 kb on + strand at 5117.819 kb on - strand at 5117.858 kb on - strand at 5117.914 kb on + strand at 5117.921 kb on + strand at 5117.928 kb on + strand at 5117.945 kb on + strand at 5117.945 kb on + strand at 5117.950 kb on + strand at 5117.951 kb on - strand at 5117.952 kb on + strand at 5117.971 kb on + strand at 5118.077 kb on - strand at 5118.077 kb on - strand at 5118.077 kb on - strand at 5118.273 kb on + strand, within BT3942 at 5118.273 kb on + strand, within BT3942 at 5118.328 kb on + strand, within BT3942 at 5118.362 kb on - strand, within BT3942 at 5118.463 kb on - strand at 5118.532 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 5,116,361 + +0.0 5,116,361 + -1.0 5,116,419 + +1.0 5,116,465 - +0.6 5,116,465 - -1.8 5,116,465 - -1.0 5,116,473 + -0.8 5,116,519 + +0.0 5,116,519 + -2.4 5,116,520 - -3.2 5,116,520 - +2.0 5,116,542 - -0.7 5,116,564 - +2.0 5,116,586 + +0.6 5,116,587 - -2.0 5,116,587 - -0.2 5,116,654 - +1.0 5,116,794 - BT3940 0.44 +0.0 5,116,931 - -1.2 5,116,940 - -2.0 5,116,940 - -1.5 5,116,956 - -1.8 5,117,013 + +1.6 5,117,066 + BT3941 0.13 -3.0 5,117,103 - BT3941 0.17 -2.7 5,117,103 - BT3941 0.17 -1.9 5,117,148 - BT3941 0.22 +1.0 5,117,196 + BT3941 0.27 -1.0 5,117,199 + BT3941 0.28 -2.3 5,117,200 - BT3941 0.28 -1.0 5,117,200 - BT3941 0.28 +0.0 5,117,201 + BT3941 0.28 -2.3 5,117,202 - BT3941 0.28 -0.8 5,117,202 - BT3941 0.28 +1.0 5,117,211 - BT3941 0.29 -0.3 5,117,325 + BT3941 0.41 -1.4 5,117,501 - BT3941 0.60 +0.0 5,117,545 + BT3941 0.65 -4.3 5,117,545 + BT3941 0.65 -3.2 5,117,546 - BT3941 0.65 +1.6 5,117,575 - BT3941 0.68 -1.0 5,117,601 + BT3941 0.71 -2.3 5,117,638 - BT3941 0.75 +0.4 5,117,818 + -1.6 5,117,818 + -3.2 5,117,819 - -2.1 5,117,858 - +1.0 5,117,914 + -0.9 5,117,921 + -1.7 5,117,928 + -2.0 5,117,945 + +0.4 5,117,945 + -1.8 5,117,950 + -1.6 5,117,951 - -0.4 5,117,952 + -0.6 5,117,971 + -1.4 5,118,077 - -1.3 5,118,077 - -2.0 5,118,077 - +0.0 5,118,273 + BT3942 0.49 -0.9 5,118,273 + BT3942 0.49 +0.0 5,118,328 + BT3942 0.62 -3.4 5,118,362 - BT3942 0.69 -2.6 5,118,463 - -0.8 5,118,532 + -2.3
Or see this region's nucleotide sequence