Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2370 and BT2371 are separated by 144 nucleotides BT2371 and BT2372 are separated by 226 nucleotides BT2372 and BT2373 are separated by 181 nucleotides
BT2370: BT2370 - hypothetical protein (NCBI ptt file), at 2,956,038 to 2,956,508
BT2370
BT2371: BT2371 - putative protease/amidase (NCBI ptt file), at 2,956,653 to 2,957,231
BT2371
BT2372: BT2372 - transcriptional regulator (NCBI ptt file), at 2,957,458 to 2,958,405
BT2372
BT2373: BT2373 - hypothetical protein (NCBI ptt file), at 2,958,587 to 2,960,149
BT2373
Position (kb)
2957
2958
2959 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2956.459 kb on - strand, within BT2370 at 2956.463 kb on - strand at 2956.521 kb on + strand at 2956.521 kb on + strand at 2956.529 kb on - strand at 2956.529 kb on - strand at 2956.530 kb on + strand at 2956.530 kb on + strand at 2956.531 kb on - strand at 2956.546 kb on + strand at 2956.556 kb on - strand at 2956.603 kb on + strand at 2956.603 kb on + strand at 2956.612 kb on - strand at 2956.626 kb on - strand at 2956.751 kb on - strand, within BT2371 at 2956.751 kb on - strand, within BT2371 at 2956.761 kb on + strand, within BT2371 at 2956.761 kb on + strand, within BT2371 at 2956.763 kb on + strand, within BT2371 at 2956.797 kb on + strand, within BT2371 at 2956.849 kb on + strand, within BT2371 at 2956.886 kb on + strand, within BT2371 at 2956.906 kb on - strand, within BT2371 at 2956.976 kb on - strand, within BT2371 at 2957.033 kb on - strand, within BT2371 at 2957.064 kb on - strand, within BT2371 at 2957.084 kb on + strand, within BT2371 at 2957.085 kb on - strand, within BT2371 at 2957.208 kb on - strand at 2957.223 kb on + strand at 2957.267 kb on - strand at 2957.345 kb on + strand at 2957.345 kb on + strand at 2957.371 kb on - strand at 2957.403 kb on + strand at 2957.432 kb on - strand at 2957.463 kb on + strand at 2957.463 kb on + strand at 2957.464 kb on - strand at 2957.465 kb on + strand at 2957.465 kb on + strand at 2957.465 kb on + strand at 2957.466 kb on - strand at 2957.529 kb on - strand at 2957.619 kb on - strand, within BT2372 at 2957.688 kb on + strand, within BT2372 at 2957.718 kb on + strand, within BT2372 at 2957.738 kb on + strand, within BT2372 at 2957.838 kb on + strand, within BT2372 at 2957.867 kb on - strand, within BT2372 at 2957.886 kb on - strand, within BT2372 at 2957.889 kb on - strand, within BT2372 at 2957.889 kb on - strand, within BT2372 at 2957.992 kb on - strand, within BT2372 at 2957.992 kb on - strand, within BT2372 at 2958.033 kb on + strand, within BT2372 at 2958.062 kb on - strand, within BT2372 at 2958.126 kb on + strand, within BT2372 at 2958.147 kb on + strand, within BT2372 at 2958.147 kb on + strand, within BT2372 at 2958.148 kb on - strand, within BT2372 at 2958.214 kb on + strand, within BT2372 at 2958.215 kb on - strand, within BT2372 at 2958.318 kb on + strand at 2958.318 kb on + strand at 2958.319 kb on - strand at 2958.322 kb on + strand at 2958.322 kb on + strand at 2958.322 kb on + strand at 2958.331 kb on + strand at 2958.379 kb on - strand at 2958.513 kb on + strand at 2958.573 kb on + strand at 2958.606 kb on - strand at 2958.606 kb on - strand at 2958.613 kb on + strand at 2958.614 kb on - strand at 2958.654 kb on + strand at 2958.655 kb on - strand at 2958.655 kb on - strand at 2958.677 kb on + strand at 2958.742 kb on + strand at 2958.743 kb on - strand at 2958.743 kb on - strand at 2958.750 kb on - strand, within BT2373 at 2958.828 kb on + strand, within BT2373 at 2958.829 kb on - strand, within BT2373 at 2958.832 kb on + strand, within BT2373 at 2958.833 kb on - strand, within BT2373 at 2958.910 kb on - strand, within BT2373 at 2958.938 kb on + strand, within BT2373 at 2959.017 kb on - strand, within BT2373 at 2959.083 kb on - strand, within BT2373 at 2959.148 kb on - strand, within BT2373 at 2959.284 kb on + strand, within BT2373 at 2959.333 kb on - strand, within BT2373 at 2959.344 kb on - strand, within BT2373
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 2,956,459 - BT2370 0.89 +0.0 2,956,463 - -0.9 2,956,521 + +0.3 2,956,521 + -2.8 2,956,529 - -0.4 2,956,529 - -3.4 2,956,530 + +0.0 2,956,530 + -0.9 2,956,531 - -1.4 2,956,546 + -0.7 2,956,556 - +0.0 2,956,603 + -0.5 2,956,603 + -0.5 2,956,612 - -1.5 2,956,626 - -1.6 2,956,751 - BT2371 0.17 -2.2 2,956,751 - BT2371 0.17 -0.8 2,956,761 + BT2371 0.19 -0.8 2,956,761 + BT2371 0.19 -2.3 2,956,763 + BT2371 0.19 +2.2 2,956,797 + BT2371 0.25 -1.5 2,956,849 + BT2371 0.34 +0.0 2,956,886 + BT2371 0.40 -2.7 2,956,906 - BT2371 0.44 -2.0 2,956,976 - BT2371 0.56 -0.7 2,957,033 - BT2371 0.66 -2.3 2,957,064 - BT2371 0.71 +1.0 2,957,084 + BT2371 0.74 -1.0 2,957,085 - BT2371 0.75 -3.2 2,957,208 - +0.0 2,957,223 + -2.4 2,957,267 - -1.0 2,957,345 + -2.5 2,957,345 + -1.0 2,957,371 - -2.3 2,957,403 + +0.0 2,957,432 - -2.2 2,957,463 + -1.6 2,957,463 + +3.2 2,957,464 - -2.3 2,957,465 + -3.0 2,957,465 + +0.0 2,957,465 + -0.8 2,957,466 - -1.7 2,957,529 - -2.4 2,957,619 - BT2372 0.17 -1.2 2,957,688 + BT2372 0.24 -1.0 2,957,718 + BT2372 0.27 -1.6 2,957,738 + BT2372 0.30 -1.0 2,957,838 + BT2372 0.40 +0.4 2,957,867 - BT2372 0.43 -0.8 2,957,886 - BT2372 0.45 +0.4 2,957,889 - BT2372 0.45 -1.6 2,957,889 - BT2372 0.45 +0.0 2,957,992 - BT2372 0.56 -0.9 2,957,992 - BT2372 0.56 +1.0 2,958,033 + BT2372 0.61 +2.0 2,958,062 - BT2372 0.64 -0.8 2,958,126 + BT2372 0.70 -1.0 2,958,147 + BT2372 0.73 -0.2 2,958,147 + BT2372 0.73 +0.4 2,958,148 - BT2372 0.73 -1.6 2,958,214 + BT2372 0.80 +2.3 2,958,215 - BT2372 0.80 +0.0 2,958,318 + +2.4 2,958,318 + -3.1 2,958,319 - -1.5 2,958,322 + +1.0 2,958,322 + +2.0 2,958,322 + +1.5 2,958,331 + +0.3 2,958,379 - -1.1 2,958,513 + +2.2 2,958,573 + -1.2 2,958,606 - +0.0 2,958,606 - +1.8 2,958,613 + +0.6 2,958,614 - -2.4 2,958,654 + -2.8 2,958,655 - -1.0 2,958,655 - -1.1 2,958,677 + -2.7 2,958,742 + -4.0 2,958,743 - -0.8 2,958,743 - +0.5 2,958,750 - BT2373 0.10 -0.6 2,958,828 + BT2373 0.15 -1.8 2,958,829 - BT2373 0.15 -1.2 2,958,832 + BT2373 0.16 +1.7 2,958,833 - BT2373 0.16 -0.6 2,958,910 - BT2373 0.21 -1.9 2,958,938 + BT2373 0.22 -0.6 2,959,017 - BT2373 0.28 -2.3 2,959,083 - BT2373 0.32 -2.1 2,959,148 - BT2373 0.36 +0.4 2,959,284 + BT2373 0.45 -0.1 2,959,333 - BT2373 0.48 +1.5 2,959,344 - BT2373 0.48 -3.3
Or see this region's nucleotide sequence