Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0472 and BT0473 are separated by 9 nucleotides BT0473 and BT0474 are separated by 30 nucleotides BT0474 and BT0475 are separated by 13 nucleotides BT0475 and BT0476 are separated by 4 nucleotides
BT0472: BT0472 - putative acyltransferase in colanic acid biosynthesis (NCBI ptt file), at 583,980 to 584,738
BT0472
BT0473: BT0473 - putative glycosyltransferase (NCBI ptt file), at 584,748 to 585,689
BT0473
BT0474: BT0474 - D-glycero-D-manno-heptose 1-phosphate kinase (NCBI ptt file), at 585,720 to 586,766
BT0474
BT0475: BT0475 - putative phosphoheptose isomerase (NCBI ptt file), at 586,780 to 587,373
BT0475
BT0476: BT0476 - D-mannose-1-phosphate guanyltransferase (NCBI ptt file), at 587,378 to 588,085
BT0476
Position (kb) 585
586
587 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 584.722 kb on - strand at 584.868 kb on - strand, within BT0473 at 584.880 kb on - strand, within BT0473 at 584.984 kb on - strand, within BT0473 at 585.240 kb on - strand, within BT0473 at 585.255 kb on - strand, within BT0473 at 585.311 kb on - strand, within BT0473 at 585.315 kb on - strand, within BT0473 at 585.417 kb on - strand, within BT0473 at 585.578 kb on - strand, within BT0473 at 585.640 kb on - strand at 585.705 kb on - strand at 585.789 kb on - strand at 585.841 kb on - strand, within BT0474 at 585.841 kb on - strand, within BT0474 at 585.849 kb on - strand, within BT0474 at 586.027 kb on - strand, within BT0474 at 586.068 kb on - strand, within BT0474 at 586.121 kb on - strand, within BT0474 at 586.201 kb on - strand, within BT0474 at 586.228 kb on - strand, within BT0474 at 586.230 kb on - strand, within BT0474 at 586.356 kb on - strand, within BT0474 at 586.416 kb on - strand, within BT0474 at 586.650 kb on - strand, within BT0474 at 586.673 kb on - strand at 586.710 kb on - strand at 586.771 kb on - strand at 587.038 kb on - strand, within BT0475 at 587.038 kb on - strand, within BT0475 at 587.188 kb on - strand, within BT0475 at 587.323 kb on - strand at 587.439 kb on + strand at 587.488 kb on - strand, within BT0476 at 587.607 kb on - strand, within BT0476
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 584,722 - +2.6 584,868 - BT0473 0.13 -1.6 584,880 - BT0473 0.14 -2.0 584,984 - BT0473 0.25 +1.0 585,240 - BT0473 0.52 +0.0 585,255 - BT0473 0.54 -3.1 585,311 - BT0473 0.60 -3.3 585,315 - BT0473 0.60 +0.0 585,417 - BT0473 0.71 -1.3 585,578 - BT0473 0.88 -1.0 585,640 - -1.0 585,705 - -1.9 585,789 - +0.0 585,841 - BT0474 0.12 +1.0 585,841 - BT0474 0.12 +0.0 585,849 - BT0474 0.12 +2.8 586,027 - BT0474 0.29 +1.8 586,068 - BT0474 0.33 +0.0 586,121 - BT0474 0.38 +1.6 586,201 - BT0474 0.46 +0.6 586,228 - BT0474 0.49 -2.3 586,230 - BT0474 0.49 -1.2 586,356 - BT0474 0.61 +1.0 586,416 - BT0474 0.66 -3.0 586,650 - BT0474 0.89 -2.0 586,673 - +3.5 586,710 - +0.0 586,771 - +2.1 587,038 - BT0475 0.43 -2.3 587,038 - BT0475 0.43 +0.8 587,188 - BT0475 0.69 -1.6 587,323 - -2.4 587,439 + -2.3 587,488 - BT0476 0.16 -1.6 587,607 - BT0476 0.32 -2.8
Or see this region's nucleotide sequence