Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0471 and BT0472 are separated by 7 nucleotides BT0472 and BT0473 are separated by 9 nucleotides BT0473 and BT0474 are separated by 30 nucleotides
BT0471: BT0471 - glycosyltransferase (NCBI ptt file), at 582,758 to 583,972
BT0471
BT0472: BT0472 - putative acyltransferase in colanic acid biosynthesis (NCBI ptt file), at 583,980 to 584,738
BT0472
BT0473: BT0473 - putative glycosyltransferase (NCBI ptt file), at 584,748 to 585,689
BT0473
BT0474: BT0474 - D-glycero-D-manno-heptose 1-phosphate kinase (NCBI ptt file), at 585,720 to 586,766
BT0474
Position (kb) 584
585
586 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 583.751 kb on - strand, within BT0471 at 583.764 kb on + strand, within BT0471 at 583.765 kb on - strand, within BT0471 at 583.950 kb on - strand at 584.004 kb on - strand at 584.284 kb on - strand, within BT0472 at 584.321 kb on - strand, within BT0472 at 584.323 kb on - strand, within BT0472 at 584.422 kb on - strand, within BT0472 at 584.451 kb on - strand, within BT0472 at 584.451 kb on - strand, within BT0472 at 584.564 kb on - strand, within BT0472 at 584.651 kb on - strand, within BT0472 at 584.722 kb on - strand at 584.868 kb on - strand, within BT0473 at 584.880 kb on - strand, within BT0473 at 584.984 kb on - strand, within BT0473 at 585.240 kb on - strand, within BT0473 at 585.255 kb on - strand, within BT0473 at 585.311 kb on - strand, within BT0473 at 585.315 kb on - strand, within BT0473 at 585.417 kb on - strand, within BT0473 at 585.578 kb on - strand, within BT0473 at 585.640 kb on - strand at 585.705 kb on - strand at 585.789 kb on - strand at 585.841 kb on - strand, within BT0474 at 585.841 kb on - strand, within BT0474 at 585.849 kb on - strand, within BT0474 at 586.027 kb on - strand, within BT0474 at 586.068 kb on - strand, within BT0474 at 586.121 kb on - strand, within BT0474 at 586.201 kb on - strand, within BT0474 at 586.228 kb on - strand, within BT0474 at 586.230 kb on - strand, within BT0474 at 586.356 kb on - strand, within BT0474 at 586.416 kb on - strand, within BT0474 at 586.650 kb on - strand, within BT0474 at 586.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 583,751 - BT0471 0.82 -0.2 583,764 + BT0471 0.83 -1.6 583,765 - BT0471 0.83 +2.4 583,950 - -1.0 584,004 - -1.0 584,284 - BT0472 0.40 +0.4 584,321 - BT0472 0.45 -2.8 584,323 - BT0472 0.45 -0.7 584,422 - BT0472 0.58 +0.0 584,451 - BT0472 0.62 -1.0 584,451 - BT0472 0.62 -0.9 584,564 - BT0472 0.77 +0.0 584,651 - BT0472 0.88 +1.0 584,722 - +2.6 584,868 - BT0473 0.13 -1.6 584,880 - BT0473 0.14 -2.0 584,984 - BT0473 0.25 +1.0 585,240 - BT0473 0.52 +0.0 585,255 - BT0473 0.54 -3.1 585,311 - BT0473 0.60 -3.3 585,315 - BT0473 0.60 +0.0 585,417 - BT0473 0.71 -1.3 585,578 - BT0473 0.88 -1.0 585,640 - -1.0 585,705 - -1.9 585,789 - +0.0 585,841 - BT0474 0.12 +1.0 585,841 - BT0474 0.12 +0.0 585,849 - BT0474 0.12 +2.8 586,027 - BT0474 0.29 +1.8 586,068 - BT0474 0.33 +0.0 586,121 - BT0474 0.38 +1.6 586,201 - BT0474 0.46 +0.6 586,228 - BT0474 0.49 -2.3 586,230 - BT0474 0.49 -1.2 586,356 - BT0474 0.61 +1.0 586,416 - BT0474 0.66 -3.0 586,650 - BT0474 0.89 -2.0 586,673 - +3.5
Or see this region's nucleotide sequence