Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0289 and BT0290 are separated by 317 nucleotides BT0290 and BT0291 are separated by 202 nucleotides
BT0289: BT0289 - conserved hypothetical protein (NCBI ptt file), at 334,208 to 336,898
BT0289
BT0290: BT0290 - beta-galactosidase precursor (NCBI ptt file), at 337,216 to 339,555
BT0290
BT0291: BT0291 - integrase (NCBI ptt file), at 339,758 to 340,723
BT0291
Position (kb)
337
338
339
340 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 336.242 kb on - strand, within BT0289 at 336.405 kb on - strand, within BT0289 at 336.475 kb on + strand, within BT0289 at 336.479 kb on - strand, within BT0289 at 336.480 kb on + strand, within BT0289 at 336.491 kb on - strand, within BT0289 at 336.570 kb on - strand, within BT0289 at 336.579 kb on - strand, within BT0289 at 336.620 kb on - strand, within BT0289 at 336.720 kb on - strand at 336.728 kb on - strand at 336.752 kb on + strand at 336.797 kb on - strand at 336.845 kb on - strand at 336.853 kb on - strand at 336.878 kb on + strand at 336.990 kb on + strand at 336.990 kb on + strand at 337.045 kb on + strand at 337.046 kb on - strand at 337.052 kb on - strand at 337.180 kb on - strand at 337.193 kb on - strand at 337.193 kb on - strand at 337.210 kb on + strand at 337.211 kb on - strand at 337.229 kb on + strand at 337.234 kb on + strand at 337.315 kb on - strand at 337.570 kb on + strand, within BT0290 at 337.570 kb on + strand, within BT0290 at 337.657 kb on + strand, within BT0290 at 337.763 kb on - strand, within BT0290 at 337.794 kb on - strand, within BT0290 at 337.815 kb on - strand, within BT0290 at 337.845 kb on - strand, within BT0290 at 338.057 kb on + strand, within BT0290 at 338.104 kb on + strand, within BT0290 at 338.183 kb on - strand, within BT0290 at 338.183 kb on - strand, within BT0290 at 338.240 kb on + strand, within BT0290 at 338.261 kb on - strand, within BT0290 at 338.310 kb on + strand, within BT0290 at 338.312 kb on + strand, within BT0290 at 338.312 kb on + strand, within BT0290 at 338.313 kb on - strand, within BT0290 at 338.328 kb on + strand, within BT0290 at 338.359 kb on - strand, within BT0290 at 338.427 kb on + strand, within BT0290 at 338.562 kb on - strand, within BT0290 at 338.564 kb on + strand, within BT0290 at 338.565 kb on - strand, within BT0290 at 338.644 kb on - strand, within BT0290 at 338.723 kb on + strand, within BT0290 at 338.723 kb on + strand, within BT0290 at 338.723 kb on + strand, within BT0290 at 338.725 kb on + strand, within BT0290 at 338.725 kb on + strand, within BT0290 at 338.768 kb on - strand, within BT0290 at 338.869 kb on - strand, within BT0290 at 338.875 kb on - strand, within BT0290 at 338.938 kb on + strand, within BT0290 at 338.939 kb on - strand, within BT0290 at 338.939 kb on - strand, within BT0290 at 338.939 kb on - strand, within BT0290 at 338.983 kb on - strand, within BT0290 at 339.021 kb on - strand, within BT0290 at 339.067 kb on + strand, within BT0290 at 339.135 kb on + strand, within BT0290 at 339.395 kb on - strand at 339.395 kb on - strand at 339.472 kb on + strand at 339.477 kb on - strand at 339.510 kb on + strand at 339.589 kb on - strand at 339.737 kb on - strand at 339.745 kb on - strand at 339.745 kb on - strand at 339.749 kb on - strand at 339.755 kb on + strand at 339.846 kb on - strand at 339.889 kb on + strand, within BT0291 at 339.890 kb on - strand, within BT0291 at 339.909 kb on + strand, within BT0291 at 339.970 kb on + strand, within BT0291 at 339.986 kb on - strand, within BT0291 at 339.991 kb on + strand, within BT0291 at 339.991 kb on + strand, within BT0291 at 340.070 kb on - strand, within BT0291 at 340.201 kb on + strand, within BT0291 at 340.303 kb on - strand, within BT0291 at 340.373 kb on + strand, within BT0291 at 340.377 kb on + strand, within BT0291 at 340.514 kb on + strand, within BT0291
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 336,242 - BT0289 0.76 -1.0 336,405 - BT0289 0.82 -0.8 336,475 + BT0289 0.84 -0.5 336,479 - BT0289 0.84 -1.0 336,480 + BT0289 0.84 -0.0 336,491 - BT0289 0.85 -1.6 336,570 - BT0289 0.88 -1.6 336,579 - BT0289 0.88 +1.2 336,620 - BT0289 0.90 -1.7 336,720 - -2.0 336,728 - -0.6 336,752 + -2.6 336,797 - +0.4 336,845 - -0.0 336,853 - -0.7 336,878 + -1.0 336,990 + +0.7 336,990 + +0.6 337,045 + -1.4 337,046 - +0.5 337,052 - +2.1 337,180 - +0.4 337,193 - -1.3 337,193 - -1.0 337,210 + -0.6 337,211 - -1.4 337,229 + -1.0 337,234 + -3.3 337,315 - -0.0 337,570 + BT0290 0.15 +1.0 337,570 + BT0290 0.15 -1.6 337,657 + BT0290 0.19 +3.1 337,763 - BT0290 0.23 -0.6 337,794 - BT0290 0.25 -0.3 337,815 - BT0290 0.26 -1.2 337,845 - BT0290 0.27 -0.0 338,057 + BT0290 0.36 +0.7 338,104 + BT0290 0.38 -2.0 338,183 - BT0290 0.41 -3.5 338,183 - BT0290 0.41 -1.0 338,240 + BT0290 0.44 -0.0 338,261 - BT0290 0.45 +0.7 338,310 + BT0290 0.47 +1.0 338,312 + BT0290 0.47 +3.0 338,312 + BT0290 0.47 -0.8 338,313 - BT0290 0.47 -1.0 338,328 + BT0290 0.48 -0.0 338,359 - BT0290 0.49 -0.4 338,427 + BT0290 0.52 -1.0 338,562 - BT0290 0.58 +0.6 338,564 + BT0290 0.58 +1.2 338,565 - BT0290 0.58 +0.4 338,644 - BT0290 0.61 -0.8 338,723 + BT0290 0.64 -0.7 338,723 + BT0290 0.64 +2.0 338,723 + BT0290 0.64 -1.3 338,725 + BT0290 0.64 -2.8 338,725 + BT0290 0.64 +1.0 338,768 - BT0290 0.66 -0.8 338,869 - BT0290 0.71 -1.6 338,875 - BT0290 0.71 -0.0 338,938 + BT0290 0.74 +1.0 338,939 - BT0290 0.74 -3.5 338,939 - BT0290 0.74 +2.3 338,939 - BT0290 0.74 -0.2 338,983 - BT0290 0.76 -1.6 339,021 - BT0290 0.77 -4.0 339,067 + BT0290 0.79 -0.0 339,135 + BT0290 0.82 +0.6 339,395 - -1.4 339,395 - -0.9 339,472 + -0.8 339,477 - +1.0 339,510 + +1.2 339,589 - +0.0 339,737 - +1.5 339,745 - +2.6 339,745 - -0.3 339,749 - -0.3 339,755 + -0.0 339,846 - -0.6 339,889 + BT0291 0.14 -2.2 339,890 - BT0291 0.14 +1.6 339,909 + BT0291 0.16 -1.0 339,970 + BT0291 0.22 +0.9 339,986 - BT0291 0.24 -2.6 339,991 + BT0291 0.24 -0.2 339,991 + BT0291 0.24 +0.2 340,070 - BT0291 0.32 -0.8 340,201 + BT0291 0.46 -0.6 340,303 - BT0291 0.56 -3.0 340,373 + BT0291 0.64 -2.0 340,377 + BT0291 0.64 +2.3 340,514 + BT0291 0.78 -3.3
Or see this region's nucleotide sequence