Experiment: Pyruvate-Sulfate (60-15mM) with Tungstate 2mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3147 and malQ are separated by 288 nucleotides malQ and sppA are separated by 208 nucleotides
DVU3147: DVU3147 - 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (TIGR), at 3,297,454 to 3,298,716
DVU3147
DVU3148: malQ - 4-alpha-glucanotransferase (TIGR), at 3,299,005 to 3,300,516
malQ
DVU3149: sppA - signal peptide peptidase SppA, 36K type (TIGR), at 3,300,725 to 3,301,615
sppA
Position (kb)
3299
3300
3301 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3298.010 kb on - strand, within DVU3147 at 3298.012 kb on + strand, within DVU3147 at 3298.032 kb on + strand, within DVU3147 at 3298.123 kb on + strand, within DVU3147 at 3298.285 kb on - strand, within DVU3147 at 3298.287 kb on + strand, within DVU3147 at 3298.441 kb on + strand, within DVU3147 at 3298.471 kb on + strand, within DVU3147 at 3298.491 kb on - strand, within DVU3147 at 3298.606 kb on - strand at 3298.651 kb on - strand at 3298.804 kb on + strand at 3298.816 kb on + strand at 3298.818 kb on + strand at 3298.854 kb on - strand at 3298.903 kb on - strand at 3298.925 kb on + strand at 3298.991 kb on - strand at 3299.020 kb on - strand at 3299.108 kb on - strand at 3299.167 kb on + strand, within malQ at 3299.174 kb on - strand, within malQ at 3299.193 kb on - strand, within malQ at 3299.208 kb on - strand, within malQ at 3299.220 kb on + strand, within malQ at 3299.278 kb on - strand, within malQ at 3299.319 kb on + strand, within malQ at 3299.350 kb on + strand, within malQ at 3299.429 kb on - strand, within malQ at 3299.456 kb on - strand, within malQ at 3299.456 kb on - strand, within malQ at 3299.524 kb on - strand, within malQ at 3299.525 kb on + strand, within malQ at 3299.526 kb on - strand, within malQ at 3299.531 kb on - strand, within malQ at 3299.542 kb on + strand, within malQ at 3299.679 kb on - strand, within malQ at 3299.717 kb on + strand, within malQ at 3299.725 kb on - strand, within malQ at 3299.791 kb on - strand, within malQ at 3299.791 kb on - strand, within malQ at 3299.821 kb on - strand, within malQ at 3299.828 kb on - strand, within malQ at 3299.830 kb on + strand, within malQ at 3299.906 kb on - strand, within malQ at 3299.952 kb on - strand, within malQ at 3300.083 kb on - strand, within malQ at 3300.083 kb on - strand, within malQ at 3300.096 kb on - strand, within malQ at 3300.140 kb on - strand, within malQ at 3300.213 kb on - strand, within malQ at 3300.301 kb on + strand, within malQ at 3300.318 kb on - strand, within malQ at 3300.322 kb on + strand, within malQ at 3300.357 kb on + strand, within malQ at 3300.516 kb on + strand at 3300.665 kb on - strand at 3300.755 kb on + strand at 3300.927 kb on - strand, within sppA at 3301.096 kb on + strand, within sppA at 3301.190 kb on - strand, within sppA at 3301.192 kb on - strand, within sppA at 3301.344 kb on + strand, within sppA at 3301.367 kb on + strand, within sppA at 3301.375 kb on - strand, within sppA
Per-strain Table
Position Strand Gene LocusTag Fraction Pyruvate-Sulfate (60-15mM) with Tungstate 2mM remove 3,298,010 - DVU3147 0.44 -0.9 3,298,012 + DVU3147 0.44 -1.0 3,298,032 + DVU3147 0.46 -0.1 3,298,123 + DVU3147 0.53 -0.6 3,298,285 - DVU3147 0.66 -0.7 3,298,287 + DVU3147 0.66 -1.2 3,298,441 + DVU3147 0.78 -1.3 3,298,471 + DVU3147 0.81 -1.0 3,298,491 - DVU3147 0.82 +0.0 3,298,606 - +1.1 3,298,651 - +1.1 3,298,804 + -3.1 3,298,816 + -0.9 3,298,818 + +0.4 3,298,854 - +1.3 3,298,903 - +0.8 3,298,925 + -0.4 3,298,991 - -0.5 3,299,020 - -0.8 3,299,108 - +0.2 3,299,167 + malQ DVU3148 0.11 +0.2 3,299,174 - malQ DVU3148 0.11 +0.2 3,299,193 - malQ DVU3148 0.12 -1.2 3,299,208 - malQ DVU3148 0.13 +1.8 3,299,220 + malQ DVU3148 0.14 -0.1 3,299,278 - malQ DVU3148 0.18 -0.1 3,299,319 + malQ DVU3148 0.21 +0.2 3,299,350 + malQ DVU3148 0.23 -0.6 3,299,429 - malQ DVU3148 0.28 -1.2 3,299,456 - malQ DVU3148 0.30 -0.1 3,299,456 - malQ DVU3148 0.30 +0.3 3,299,524 - malQ DVU3148 0.34 -0.8 3,299,525 + malQ DVU3148 0.34 -0.2 3,299,526 - malQ DVU3148 0.34 +1.2 3,299,531 - malQ DVU3148 0.35 +0.7 3,299,542 + malQ DVU3148 0.36 +0.2 3,299,679 - malQ DVU3148 0.45 +0.3 3,299,717 + malQ DVU3148 0.47 -0.2 3,299,725 - malQ DVU3148 0.48 -0.2 3,299,791 - malQ DVU3148 0.52 -0.6 3,299,791 - malQ DVU3148 0.52 -1.0 3,299,821 - malQ DVU3148 0.54 -1.0 3,299,828 - malQ DVU3148 0.54 +0.1 3,299,830 + malQ DVU3148 0.55 +0.4 3,299,906 - malQ DVU3148 0.60 -0.5 3,299,952 - malQ DVU3148 0.63 -0.1 3,300,083 - malQ DVU3148 0.71 +0.4 3,300,083 - malQ DVU3148 0.71 -1.6 3,300,096 - malQ DVU3148 0.72 +0.2 3,300,140 - malQ DVU3148 0.75 -0.1 3,300,213 - malQ DVU3148 0.80 +1.8 3,300,301 + malQ DVU3148 0.86 +0.4 3,300,318 - malQ DVU3148 0.87 +0.0 3,300,322 + malQ DVU3148 0.87 -0.4 3,300,357 + malQ DVU3148 0.89 +0.0 3,300,516 + -0.4 3,300,665 - -1.6 3,300,755 + +0.1 3,300,927 - sppA DVU3149 0.23 +0.8 3,301,096 + sppA DVU3149 0.42 -1.2 3,301,190 - sppA DVU3149 0.52 +0.6 3,301,192 - sppA DVU3149 0.52 -0.9 3,301,344 + sppA DVU3149 0.69 -0.8 3,301,367 + sppA DVU3149 0.72 -0.7 3,301,375 - sppA DVU3149 0.73 -1.1
Or see this region's nucleotide sequence