Experiment: KB with Prenyletin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1638 and Psyr_1639 are separated by 669 nucleotides Psyr_1639 and Psyr_1640 overlap by 11 nucleotides Psyr_1640 and Psyr_1641 are separated by 24 nucleotides
Psyr_1638: Psyr_1638 - RNAse E, at 1,842,286 to 1,845,648
_1638
Psyr_1639: Psyr_1639 - ribosomal large subunit pseudouridine synthase C, at 1,846,318 to 1,847,274
_1639
Psyr_1640: Psyr_1640 - HAD-superfamily hydrolase, subfamily IA, variant 3:HAD-superfamily hydrolase, subfamily IA, variant 1, at 1,847,264 to 1,847,926
_1640
Psyr_1641: Psyr_1641 - Peptidase S49, SppA, at 1,847,951 to 1,848,949
_1641
Position (kb)
1846
1847
1848 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1846.132 kb on - strand at 1846.265 kb on - strand at 1846.265 kb on - strand at 1846.265 kb on - strand at 1846.265 kb on - strand at 1846.265 kb on - strand at 1846.265 kb on - strand at 1846.278 kb on - strand at 1846.278 kb on - strand at 1846.306 kb on - strand at 1846.411 kb on + strand at 1846.412 kb on - strand at 1846.499 kb on - strand, within Psyr_1639 at 1846.499 kb on - strand, within Psyr_1639 at 1846.612 kb on + strand, within Psyr_1639 at 1846.613 kb on - strand, within Psyr_1639 at 1846.613 kb on - strand, within Psyr_1639 at 1846.630 kb on - strand, within Psyr_1639 at 1846.654 kb on - strand, within Psyr_1639 at 1846.761 kb on + strand, within Psyr_1639 at 1846.761 kb on + strand, within Psyr_1639 at 1846.761 kb on + strand, within Psyr_1639 at 1846.849 kb on + strand, within Psyr_1639 at 1846.849 kb on + strand, within Psyr_1639 at 1846.850 kb on - strand, within Psyr_1639 at 1846.850 kb on - strand, within Psyr_1639 at 1847.090 kb on + strand, within Psyr_1639 at 1847.107 kb on + strand, within Psyr_1639 at 1847.107 kb on + strand, within Psyr_1639 at 1847.108 kb on - strand, within Psyr_1639 at 1847.108 kb on - strand, within Psyr_1639 at 1847.108 kb on - strand, within Psyr_1639 at 1847.173 kb on + strand, within Psyr_1639 at 1847.173 kb on + strand, within Psyr_1639 at 1847.173 kb on + strand, within Psyr_1639 at 1847.173 kb on + strand, within Psyr_1639 at 1847.173 kb on + strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.174 kb on - strand, within Psyr_1639 at 1847.211 kb on + strand at 1847.212 kb on - strand at 1847.212 kb on - strand at 1847.277 kb on - strand at 1847.486 kb on + strand, within Psyr_1640 at 1847.487 kb on - strand, within Psyr_1640 at 1847.487 kb on - strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.498 kb on + strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.499 kb on - strand, within Psyr_1640 at 1847.651 kb on + strand, within Psyr_1640 at 1847.651 kb on + strand, within Psyr_1640 at 1847.652 kb on - strand, within Psyr_1640 at 1847.652 kb on - strand, within Psyr_1640 at 1847.652 kb on - strand at 1848.019 kb on + strand at 1848.019 kb on + strand at 1848.020 kb on - strand at 1848.020 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Prenyletin 10 mM remove 1,846,132 - +0.6 1,846,265 - -2.3 1,846,265 - -1.2 1,846,265 - -0.2 1,846,265 - -1.5 1,846,265 - +0.6 1,846,265 - -3.3 1,846,278 - +0.6 1,846,278 - +0.0 1,846,306 - +0.9 1,846,411 + -0.1 1,846,412 - -1.2 1,846,499 - Psyr_1639 0.19 -3.7 1,846,499 - Psyr_1639 0.19 +0.8 1,846,612 + Psyr_1639 0.31 -0.4 1,846,613 - Psyr_1639 0.31 -1.5 1,846,613 - Psyr_1639 0.31 +0.9 1,846,630 - Psyr_1639 0.33 -1.1 1,846,654 - Psyr_1639 0.35 +0.0 1,846,761 + Psyr_1639 0.46 -0.2 1,846,761 + Psyr_1639 0.46 -1.0 1,846,761 + Psyr_1639 0.46 -0.1 1,846,849 + Psyr_1639 0.55 -0.6 1,846,849 + Psyr_1639 0.55 -0.8 1,846,850 - Psyr_1639 0.56 +0.0 1,846,850 - Psyr_1639 0.56 +1.8 1,847,090 + Psyr_1639 0.81 +2.0 1,847,107 + Psyr_1639 0.82 +0.0 1,847,107 + Psyr_1639 0.82 +2.4 1,847,108 - Psyr_1639 0.83 -0.6 1,847,108 - Psyr_1639 0.83 +1.2 1,847,108 - Psyr_1639 0.83 +1.6 1,847,173 + Psyr_1639 0.89 -0.5 1,847,173 + Psyr_1639 0.89 +0.0 1,847,173 + Psyr_1639 0.89 -1.4 1,847,173 + Psyr_1639 0.89 +0.5 1,847,173 + Psyr_1639 0.89 -0.4 1,847,174 - Psyr_1639 0.89 -0.4 1,847,174 - Psyr_1639 0.89 -0.1 1,847,174 - Psyr_1639 0.89 -0.2 1,847,174 - Psyr_1639 0.89 -0.4 1,847,174 - Psyr_1639 0.89 -2.0 1,847,174 - Psyr_1639 0.89 -1.7 1,847,174 - Psyr_1639 0.89 +0.0 1,847,174 - Psyr_1639 0.89 -0.5 1,847,174 - Psyr_1639 0.89 -0.2 1,847,174 - Psyr_1639 0.89 -3.1 1,847,174 - Psyr_1639 0.89 +3.8 1,847,174 - Psyr_1639 0.89 +0.9 1,847,174 - Psyr_1639 0.89 -2.2 1,847,174 - Psyr_1639 0.89 -1.8 1,847,174 - Psyr_1639 0.89 -0.8 1,847,174 - Psyr_1639 0.89 +0.3 1,847,211 + -0.8 1,847,212 - -0.8 1,847,212 - -1.1 1,847,277 - +2.6 1,847,486 + Psyr_1640 0.33 -0.6 1,847,487 - Psyr_1640 0.34 +0.6 1,847,487 - Psyr_1640 0.34 +2.0 1,847,498 + Psyr_1640 0.35 +1.0 1,847,498 + Psyr_1640 0.35 +1.6 1,847,498 + Psyr_1640 0.35 -0.6 1,847,498 + Psyr_1640 0.35 +0.2 1,847,498 + Psyr_1640 0.35 +0.6 1,847,498 + Psyr_1640 0.35 -2.8 1,847,498 + Psyr_1640 0.35 +1.4 1,847,498 + Psyr_1640 0.35 +1.4 1,847,498 + Psyr_1640 0.35 +0.1 1,847,498 + Psyr_1640 0.35 -1.4 1,847,498 + Psyr_1640 0.35 +0.9 1,847,498 + Psyr_1640 0.35 +0.0 1,847,498 + Psyr_1640 0.35 -0.9 1,847,498 + Psyr_1640 0.35 +1.6 1,847,498 + Psyr_1640 0.35 -1.1 1,847,499 - Psyr_1640 0.35 +0.3 1,847,499 - Psyr_1640 0.35 +0.6 1,847,499 - Psyr_1640 0.35 +1.0 1,847,499 - Psyr_1640 0.35 +0.6 1,847,499 - Psyr_1640 0.35 +3.0 1,847,499 - Psyr_1640 0.35 +0.0 1,847,651 + Psyr_1640 0.58 +0.6 1,847,651 + Psyr_1640 0.58 -0.6 1,847,652 - Psyr_1640 0.59 -0.4 1,847,652 - Psyr_1640 0.59 -0.6 1,847,652 - -0.6 1,848,019 + +0.5 1,848,019 + -1.1 1,848,020 - +1.2 1,848,020 - -1.2
Or see this region's nucleotide sequence