Experiment: BHIS with Dimethyl Sulfoxide 0.1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4270 and BT4271 are separated by 112 nucleotides BT4271 and BT4272 are separated by 5 nucleotides
BT4270: BT4270 - hypothetical protein (NCBI ptt file), at 5,625,894 to 5,627,711
BT4270
BT4271: BT4271 - hypothetical protein (NCBI ptt file), at 5,627,824 to 5,628,531
BT4271
BT4272: BT4272 - hypothetical protein (NCBI ptt file), at 5,628,537 to 5,631,527
BT4272
Position (kb)
5627
5628
5629 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5626.853 kb on + strand, within BT4270 at 5626.929 kb on - strand, within BT4270 at 5627.054 kb on + strand, within BT4270 at 5627.065 kb on + strand, within BT4270 at 5627.066 kb on - strand, within BT4270 at 5627.066 kb on - strand, within BT4270 at 5627.070 kb on - strand, within BT4270 at 5627.111 kb on + strand, within BT4270 at 5627.124 kb on + strand, within BT4270 at 5627.202 kb on - strand, within BT4270 at 5627.220 kb on - strand, within BT4270 at 5627.284 kb on + strand, within BT4270 at 5627.284 kb on + strand, within BT4270 at 5627.285 kb on - strand, within BT4270 at 5627.391 kb on + strand, within BT4270 at 5627.493 kb on + strand, within BT4270 at 5627.494 kb on - strand, within BT4270 at 5627.495 kb on + strand, within BT4270 at 5627.560 kb on - strand at 5627.565 kb on + strand at 5627.639 kb on + strand at 5627.653 kb on - strand at 5627.659 kb on - strand at 5627.660 kb on + strand at 5627.702 kb on - strand at 5627.721 kb on - strand at 5627.801 kb on - strand at 5627.855 kb on + strand at 5627.989 kb on + strand, within BT4271 at 5627.989 kb on + strand, within BT4271 at 5628.015 kb on + strand at 5628.068 kb on - strand, within BT4271 at 5628.068 kb on - strand, within BT4271 at 5628.180 kb on - strand, within BT4271 at 5628.205 kb on - strand, within BT4271 at 5628.205 kb on - strand, within BT4271 at 5628.398 kb on + strand, within BT4271 at 5628.471 kb on + strand at 5628.565 kb on + strand at 5628.566 kb on - strand at 5628.569 kb on + strand at 5628.570 kb on - strand at 5628.570 kb on - strand at 5628.599 kb on + strand at 5628.606 kb on + strand at 5628.627 kb on - strand at 5628.627 kb on - strand at 5628.703 kb on + strand at 5628.854 kb on + strand, within BT4272 at 5628.994 kb on - strand, within BT4272 at 5629.000 kb on + strand, within BT4272 at 5629.000 kb on + strand, within BT4272 at 5629.052 kb on + strand, within BT4272 at 5629.053 kb on - strand, within BT4272 at 5629.127 kb on + strand, within BT4272 at 5629.127 kb on + strand, within BT4272 at 5629.128 kb on - strand, within BT4272 at 5629.142 kb on - strand, within BT4272 at 5629.164 kb on + strand, within BT4272 at 5629.216 kb on + strand, within BT4272 at 5629.286 kb on + strand, within BT4272 at 5629.288 kb on + strand, within BT4272 at 5629.300 kb on - strand, within BT4272 at 5629.374 kb on + strand, within BT4272 at 5629.402 kb on + strand, within BT4272 at 5629.407 kb on + strand, within BT4272 at 5629.407 kb on + strand, within BT4272 at 5629.408 kb on - strand, within BT4272 at 5629.414 kb on + strand, within BT4272 at 5629.433 kb on + strand, within BT4272 at 5629.491 kb on - strand, within BT4272 at 5629.498 kb on + strand, within BT4272 at 5629.507 kb on - strand, within BT4272
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.1 vol% remove 5,626,853 + BT4270 0.53 -0.8 5,626,929 - BT4270 0.57 -1.8 5,627,054 + BT4270 0.64 -2.8 5,627,065 + BT4270 0.64 +0.1 5,627,066 - BT4270 0.64 -2.3 5,627,066 - BT4270 0.64 -3.1 5,627,070 - BT4270 0.65 -0.5 5,627,111 + BT4270 0.67 -2.1 5,627,124 + BT4270 0.68 -1.9 5,627,202 - BT4270 0.72 -0.9 5,627,220 - BT4270 0.73 +0.4 5,627,284 + BT4270 0.76 -0.7 5,627,284 + BT4270 0.76 -1.4 5,627,285 - BT4270 0.77 -1.6 5,627,391 + BT4270 0.82 -2.1 5,627,493 + BT4270 0.88 -1.6 5,627,494 - BT4270 0.88 -2.1 5,627,495 + BT4270 0.88 -1.7 5,627,560 - -1.0 5,627,565 + -1.5 5,627,639 + -0.6 5,627,653 - -0.4 5,627,659 - -1.1 5,627,660 + +1.0 5,627,702 - +0.0 5,627,721 - -1.2 5,627,801 - +0.0 5,627,855 + +0.0 5,627,989 + BT4271 0.23 -1.0 5,627,989 + BT4271 0.23 +0.0 5,628,015 + +0.0 5,628,068 - BT4271 0.34 -2.3 5,628,068 - BT4271 0.34 -3.4 5,628,180 - BT4271 0.50 +1.0 5,628,205 - BT4271 0.54 -2.7 5,628,205 - BT4271 0.54 -2.0 5,628,398 + BT4271 0.81 -1.3 5,628,471 + -0.5 5,628,565 + +1.0 5,628,566 - +0.8 5,628,569 + -2.3 5,628,570 - -1.8 5,628,570 - +0.0 5,628,599 + +0.0 5,628,606 + -1.6 5,628,627 - -1.4 5,628,627 - -2.6 5,628,703 + -0.8 5,628,854 + BT4272 0.11 +0.0 5,628,994 - BT4272 0.15 -2.4 5,629,000 + BT4272 0.15 -2.6 5,629,000 + BT4272 0.15 +0.0 5,629,052 + BT4272 0.17 +0.8 5,629,053 - BT4272 0.17 -0.8 5,629,127 + BT4272 0.20 -0.6 5,629,127 + BT4272 0.20 +0.0 5,629,128 - BT4272 0.20 -0.1 5,629,142 - BT4272 0.20 +0.0 5,629,164 + BT4272 0.21 +0.0 5,629,216 + BT4272 0.23 +0.0 5,629,286 + BT4272 0.25 -1.4 5,629,288 + BT4272 0.25 -1.4 5,629,300 - BT4272 0.26 -1.4 5,629,374 + BT4272 0.28 -0.2 5,629,402 + BT4272 0.29 +0.0 5,629,407 + BT4272 0.29 +0.4 5,629,407 + BT4272 0.29 -3.0 5,629,408 - BT4272 0.29 -1.4 5,629,414 + BT4272 0.29 +0.0 5,629,433 + BT4272 0.30 -1.0 5,629,491 - BT4272 0.32 -2.5 5,629,498 + BT4272 0.32 -0.4 5,629,507 - BT4272 0.32 -0.5
Or see this region's nucleotide sequence