Experiment: BHIS with Dimethyl Sulfoxide 0.1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4116 and BT4117 are separated by 283 nucleotides BT4117 and BT4118 are separated by 3 nucleotides
BT4116: BT4116 - conserved hypothetical protein (NCBI ptt file), at 5,389,689 to 5,391,260
BT4116
BT4117: BT4117 - conserved hypothetical protein (NCBI ptt file), at 5,391,544 to 5,392,338
BT4117
BT4118: BT4118 - putative two-component system response regulator, no kinase domain (NCBI ptt file), at 5,392,342 to 5,393,154
BT4118
Position (kb)
5391
5392
5393 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5390.618 kb on - strand, within BT4116 at 5390.634 kb on - strand, within BT4116 at 5390.664 kb on - strand, within BT4116 at 5390.769 kb on - strand, within BT4116 at 5390.814 kb on + strand, within BT4116 at 5390.815 kb on - strand, within BT4116 at 5390.871 kb on + strand, within BT4116 at 5391.011 kb on + strand, within BT4116 at 5391.011 kb on + strand, within BT4116 at 5391.011 kb on + strand, within BT4116 at 5391.012 kb on - strand, within BT4116 at 5391.012 kb on - strand, within BT4116 at 5391.013 kb on + strand, within BT4116 at 5391.016 kb on - strand, within BT4116 at 5391.016 kb on - strand, within BT4116 at 5391.038 kb on + strand, within BT4116 at 5391.162 kb on + strand at 5391.216 kb on + strand at 5391.216 kb on + strand at 5391.254 kb on + strand at 5391.289 kb on - strand at 5391.304 kb on + strand at 5391.305 kb on - strand at 5391.309 kb on - strand at 5391.327 kb on - strand at 5391.385 kb on - strand at 5391.395 kb on - strand at 5391.407 kb on - strand at 5391.509 kb on + strand at 5391.537 kb on - strand at 5391.574 kb on - strand at 5391.676 kb on - strand, within BT4117 at 5391.677 kb on + strand, within BT4117 at 5391.681 kb on - strand, within BT4117 at 5391.683 kb on + strand, within BT4117 at 5391.684 kb on - strand, within BT4117 at 5391.684 kb on - strand, within BT4117 at 5391.705 kb on + strand, within BT4117 at 5391.707 kb on + strand, within BT4117 at 5391.707 kb on + strand, within BT4117 at 5391.728 kb on + strand, within BT4117 at 5391.753 kb on + strand, within BT4117 at 5391.761 kb on + strand, within BT4117 at 5391.910 kb on - strand, within BT4117 at 5391.925 kb on - strand, within BT4117 at 5392.050 kb on - strand, within BT4117 at 5392.128 kb on + strand, within BT4117 at 5392.129 kb on - strand, within BT4117 at 5392.150 kb on - strand, within BT4117 at 5392.150 kb on - strand, within BT4117 at 5392.165 kb on - strand, within BT4117 at 5392.184 kb on + strand, within BT4117 at 5392.184 kb on + strand, within BT4117 at 5392.184 kb on + strand, within BT4117 at 5392.185 kb on - strand, within BT4117 at 5392.185 kb on - strand, within BT4117 at 5392.187 kb on - strand, within BT4117 at 5392.208 kb on + strand, within BT4117 at 5392.215 kb on + strand, within BT4117 at 5392.269 kb on + strand at 5392.287 kb on - strand at 5392.289 kb on + strand at 5392.342 kb on - strand at 5392.367 kb on - strand at 5392.440 kb on - strand, within BT4118 at 5392.508 kb on + strand, within BT4118 at 5392.508 kb on + strand, within BT4118 at 5392.538 kb on + strand, within BT4118 at 5392.615 kb on + strand, within BT4118 at 5392.625 kb on + strand, within BT4118 at 5392.665 kb on + strand, within BT4118 at 5392.723 kb on + strand, within BT4118 at 5392.837 kb on - strand, within BT4118 at 5392.892 kb on - strand, within BT4118 at 5392.895 kb on + strand, within BT4118 at 5392.912 kb on + strand, within BT4118 at 5392.912 kb on + strand, within BT4118 at 5392.926 kb on + strand, within BT4118 at 5392.927 kb on - strand, within BT4118 at 5392.967 kb on - strand, within BT4118 at 5393.049 kb on - strand, within BT4118 at 5393.078 kb on + strand at 5393.079 kb on - strand at 5393.153 kb on - strand at 5393.153 kb on - strand at 5393.159 kb on + strand at 5393.160 kb on - strand at 5393.160 kb on - strand at 5393.241 kb on + strand at 5393.241 kb on + strand at 5393.317 kb on - strand at 5393.317 kb on - strand at 5393.320 kb on + strand at 5393.331 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.1 vol% remove 5,390,618 - BT4116 0.59 -1.9 5,390,634 - BT4116 0.60 -1.5 5,390,664 - BT4116 0.62 -0.6 5,390,769 - BT4116 0.69 -0.5 5,390,814 + BT4116 0.72 -2.6 5,390,815 - BT4116 0.72 +1.5 5,390,871 + BT4116 0.75 -2.7 5,391,011 + BT4116 0.84 -2.6 5,391,011 + BT4116 0.84 -2.1 5,391,011 + BT4116 0.84 -0.6 5,391,012 - BT4116 0.84 -3.4 5,391,012 - BT4116 0.84 -1.3 5,391,013 + BT4116 0.84 -1.6 5,391,016 - BT4116 0.84 -0.7 5,391,016 - BT4116 0.84 -0.4 5,391,038 + BT4116 0.86 -0.0 5,391,162 + -1.3 5,391,216 + -0.0 5,391,216 + +0.4 5,391,254 + -0.0 5,391,289 - -1.2 5,391,304 + -1.9 5,391,305 - -3.0 5,391,309 - -0.3 5,391,327 - -2.3 5,391,385 - -0.0 5,391,395 - +0.5 5,391,407 - -0.0 5,391,509 + -1.6 5,391,537 - -0.0 5,391,574 - +0.4 5,391,676 - BT4117 0.17 -2.6 5,391,677 + BT4117 0.17 -1.0 5,391,681 - BT4117 0.17 -1.3 5,391,683 + BT4117 0.17 -2.0 5,391,684 - BT4117 0.18 -2.9 5,391,684 - BT4117 0.18 -1.7 5,391,705 + BT4117 0.20 -1.3 5,391,707 + BT4117 0.21 -1.1 5,391,707 + BT4117 0.21 -1.8 5,391,728 + BT4117 0.23 -2.0 5,391,753 + BT4117 0.26 -1.0 5,391,761 + BT4117 0.27 -2.0 5,391,910 - BT4117 0.46 -1.0 5,391,925 - BT4117 0.48 -1.6 5,392,050 - BT4117 0.64 -1.9 5,392,128 + BT4117 0.73 -2.3 5,392,129 - BT4117 0.74 -0.0 5,392,150 - BT4117 0.76 -2.2 5,392,150 - BT4117 0.76 +1.5 5,392,165 - BT4117 0.78 -1.8 5,392,184 + BT4117 0.81 -1.1 5,392,184 + BT4117 0.81 -1.2 5,392,184 + BT4117 0.81 -1.3 5,392,185 - BT4117 0.81 -0.4 5,392,185 - BT4117 0.81 -2.4 5,392,187 - BT4117 0.81 -2.6 5,392,208 + BT4117 0.84 -0.4 5,392,215 + BT4117 0.84 -0.0 5,392,269 + +0.2 5,392,287 - -0.0 5,392,289 + -2.6 5,392,342 - -0.0 5,392,367 - -2.2 5,392,440 - BT4118 0.12 -2.8 5,392,508 + BT4118 0.20 -1.9 5,392,508 + BT4118 0.20 -1.6 5,392,538 + BT4118 0.24 -3.5 5,392,615 + BT4118 0.34 -1.0 5,392,625 + BT4118 0.35 -1.6 5,392,665 + BT4118 0.40 -2.4 5,392,723 + BT4118 0.47 +0.3 5,392,837 - BT4118 0.61 -3.6 5,392,892 - BT4118 0.68 -3.7 5,392,895 + BT4118 0.68 -2.2 5,392,912 + BT4118 0.70 -1.0 5,392,912 + BT4118 0.70 -0.9 5,392,926 + BT4118 0.72 -1.0 5,392,927 - BT4118 0.72 -1.0 5,392,967 - BT4118 0.77 -1.6 5,393,049 - BT4118 0.87 -3.0 5,393,078 + -2.0 5,393,079 - -0.0 5,393,153 - +1.3 5,393,153 - -2.8 5,393,159 + -0.0 5,393,160 - -0.0 5,393,160 - -2.8 5,393,241 + -0.0 5,393,241 + -2.0 5,393,317 - -2.8 5,393,317 - -2.3 5,393,320 + -2.9 5,393,331 - -1.1
Or see this region's nucleotide sequence