Experiment: BHIS with Dimethyl Sulfoxide 0.1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1254 and BT1255 are separated by 124 nucleotides BT1255 and BT1256 overlap by 1 nucleotides BT1256 and BT1257 are separated by 204 nucleotides
BT1254: BT1254 - inner membrane protein (NCBI ptt file), at 1,559,674 to 1,561,029
BT1254
BT1255: BT1255 - hypothetical protein (NCBI ptt file), at 1,561,154 to 1,561,738
BT1255
BT1256: BT1256 - conserved hypothetical protein (NCBI ptt file), at 1,561,738 to 1,562,019
BT1256
BT1257: BT1257 - conserved hypothetical protein (NCBI ptt file), at 1,562,224 to 1,562,691
BT1257
Position (kb)
1561
1562 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1560.175 kb on - strand, within BT1254 at 1560.511 kb on + strand, within BT1254 at 1560.631 kb on + strand, within BT1254 at 1560.711 kb on - strand, within BT1254 at 1560.715 kb on + strand, within BT1254 at 1560.725 kb on + strand, within BT1254 at 1560.748 kb on + strand, within BT1254 at 1560.748 kb on + strand, within BT1254 at 1560.819 kb on + strand, within BT1254 at 1560.825 kb on - strand, within BT1254 at 1560.840 kb on + strand, within BT1254 at 1560.840 kb on + strand, within BT1254 at 1560.894 kb on - strand at 1560.899 kb on + strand at 1560.910 kb on + strand at 1560.911 kb on - strand at 1560.989 kb on + strand at 1560.994 kb on + strand at 1560.995 kb on - strand at 1561.038 kb on - strand at 1561.044 kb on + strand at 1561.045 kb on - strand at 1561.051 kb on + strand at 1561.097 kb on + strand at 1561.110 kb on + strand at 1561.132 kb on + strand at 1561.170 kb on - strand at 1561.369 kb on + strand, within BT1255 at 1561.369 kb on + strand, within BT1255 at 1561.370 kb on - strand, within BT1255 at 1561.467 kb on - strand, within BT1255 at 1561.473 kb on - strand, within BT1255 at 1561.615 kb on - strand, within BT1255 at 1561.641 kb on - strand, within BT1255 at 1561.641 kb on - strand, within BT1255 at 1561.641 kb on - strand, within BT1255 at 1561.652 kb on + strand, within BT1255 at 1561.653 kb on - strand, within BT1255 at 1561.799 kb on + strand, within BT1256 at 1561.800 kb on - strand, within BT1256 at 1562.020 kb on - strand at 1562.178 kb on + strand at 1562.179 kb on - strand at 1562.231 kb on + strand at 1562.236 kb on + strand at 1562.238 kb on + strand at 1562.240 kb on + strand at 1562.287 kb on - strand, within BT1257 at 1562.292 kb on + strand, within BT1257 at 1562.330 kb on + strand, within BT1257 at 1562.383 kb on + strand, within BT1257 at 1562.446 kb on + strand, within BT1257 at 1562.479 kb on + strand, within BT1257 at 1562.482 kb on - strand, within BT1257 at 1562.577 kb on - strand, within BT1257 at 1562.580 kb on + strand, within BT1257 at 1562.580 kb on + strand, within BT1257 at 1562.581 kb on - strand, within BT1257 at 1562.581 kb on - strand, within BT1257 at 1562.639 kb on + strand, within BT1257 at 1562.653 kb on - strand at 1562.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.1 vol% remove 1,560,175 - BT1254 0.37 +0.1 1,560,511 + BT1254 0.62 -0.9 1,560,631 + BT1254 0.71 +0.1 1,560,711 - BT1254 0.76 +0.1 1,560,715 + BT1254 0.77 -1.4 1,560,725 + BT1254 0.78 -0.9 1,560,748 + BT1254 0.79 -1.2 1,560,748 + BT1254 0.79 +0.1 1,560,819 + BT1254 0.84 -0.9 1,560,825 - BT1254 0.85 -1.4 1,560,840 + BT1254 0.86 -0.9 1,560,840 + BT1254 0.86 -1.0 1,560,894 - -1.8 1,560,899 + -1.3 1,560,910 + -2.6 1,560,911 - -1.1 1,560,989 + -1.3 1,560,994 + -1.3 1,560,995 - -0.3 1,561,038 - -1.9 1,561,044 + -1.5 1,561,045 - +0.1 1,561,051 + -1.1 1,561,097 + -1.3 1,561,110 + +0.1 1,561,132 + +0.1 1,561,170 - -1.5 1,561,369 + BT1255 0.37 -1.9 1,561,369 + BT1255 0.37 -1.9 1,561,370 - BT1255 0.37 -1.9 1,561,467 - BT1255 0.54 -1.4 1,561,473 - BT1255 0.55 -2.4 1,561,615 - BT1255 0.79 -0.3 1,561,641 - BT1255 0.83 +0.6 1,561,641 - BT1255 0.83 -2.3 1,561,641 - BT1255 0.83 -2.3 1,561,652 + BT1255 0.85 -3.1 1,561,653 - BT1255 0.85 -2.5 1,561,799 + BT1256 0.22 +0.1 1,561,800 - BT1256 0.22 -1.4 1,562,020 - -1.9 1,562,178 + -0.9 1,562,179 - -0.4 1,562,231 + -3.3 1,562,236 + -1.9 1,562,238 + -1.9 1,562,240 + -0.7 1,562,287 - BT1257 0.13 -0.9 1,562,292 + BT1257 0.15 +1.1 1,562,330 + BT1257 0.23 -1.5 1,562,383 + BT1257 0.34 -0.2 1,562,446 + BT1257 0.47 -0.6 1,562,479 + BT1257 0.54 -1.9 1,562,482 - BT1257 0.55 +0.6 1,562,577 - BT1257 0.75 +0.1 1,562,580 + BT1257 0.76 -0.4 1,562,580 + BT1257 0.76 -1.8 1,562,581 - BT1257 0.76 -2.5 1,562,581 - BT1257 0.76 -2.5 1,562,639 + BT1257 0.89 -0.4 1,562,653 - -0.5 1,562,731 - -0.0
Or see this region's nucleotide sequence