Experiment: MoLS4 with L-Homoserine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaE and DVU1355 are separated by 477 nucleotides DVU1355 and DVU1356 are separated by 7 nucleotides DVU1356 and DVU1357 overlap by 4 nucleotides DVU1357 and DVU1358 are separated by 218 nucleotides
DVU1353: dnaE - DNA polymerase III, alpha subunit (TIGR), at 1,430,903 to 1,434,397
dnaE
DVU1355: DVU1355 - hypothetical protein (TIGR), at 1,434,875 to 1,435,243
DVU1355
DVU1356: DVU1356 - HD domain protein (TIGR), at 1,435,251 to 1,436,054
DVU1356
DVU1357: DVU1357 - conserved domain protein (TIGR), at 1,436,051 to 1,436,677
DVU1357
DVU1358: DVU1358 - hydrolase, haloacid dehalogenase-like family (TIGR), at 1,436,896 to 1,437,588
DVU1358
Position (kb)
1435
1436
1437 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1434.464 kb on + strand at 1434.472 kb on - strand at 1434.472 kb on - strand at 1434.484 kb on + strand at 1434.484 kb on + strand at 1434.739 kb on + strand at 1434.739 kb on + strand at 1434.739 kb on + strand at 1434.747 kb on - strand at 1434.754 kb on + strand at 1434.754 kb on + strand at 1434.754 kb on + strand at 1434.762 kb on - strand at 1434.762 kb on - strand at 1434.773 kb on + strand at 1434.781 kb on - strand at 1434.956 kb on + strand, within DVU1355 at 1434.992 kb on + strand, within DVU1355 at 1435.023 kb on - strand, within DVU1355 at 1435.093 kb on - strand, within DVU1355 at 1435.167 kb on - strand, within DVU1355 at 1435.206 kb on - strand, within DVU1355 at 1435.211 kb on - strand at 1435.211 kb on - strand at 1435.265 kb on - strand at 1435.335 kb on + strand, within DVU1356 at 1435.352 kb on + strand, within DVU1356 at 1435.352 kb on + strand, within DVU1356 at 1435.360 kb on - strand, within DVU1356 at 1435.360 kb on - strand, within DVU1356 at 1435.435 kb on - strand, within DVU1356 at 1435.525 kb on + strand, within DVU1356 at 1435.625 kb on - strand, within DVU1356 at 1435.716 kb on - strand, within DVU1356 at 1435.759 kb on - strand, within DVU1356 at 1435.810 kb on + strand, within DVU1356 at 1435.823 kb on + strand, within DVU1356 at 1436.036 kb on + strand at 1436.087 kb on + strand at 1436.120 kb on + strand, within DVU1357 at 1436.659 kb on + strand at 1436.683 kb on + strand at 1436.737 kb on - strand at 1436.804 kb on - strand at 1436.810 kb on + strand at 1436.837 kb on - strand at 1436.877 kb on - strand at 1436.968 kb on + strand, within DVU1358 at 1436.976 kb on - strand, within DVU1358 at 1437.052 kb on - strand, within DVU1358
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Homoserine as nutrient 10mM remove 1,434,464 + +0.3 1,434,472 - +0.7 1,434,472 - -1.1 1,434,484 + +2.2 1,434,484 + -0.2 1,434,739 + -0.4 1,434,739 + +0.1 1,434,739 + -0.1 1,434,747 - -0.2 1,434,754 + -0.1 1,434,754 + -0.6 1,434,754 + -0.1 1,434,762 - -0.1 1,434,762 - -0.3 1,434,773 + -0.9 1,434,781 - -1.4 1,434,956 + DVU1355 0.22 -1.1 1,434,992 + DVU1355 0.32 +0.9 1,435,023 - DVU1355 0.40 +0.5 1,435,093 - DVU1355 0.59 -0.3 1,435,167 - DVU1355 0.79 -0.8 1,435,206 - DVU1355 0.90 +0.4 1,435,211 - +0.4 1,435,211 - -0.9 1,435,265 - -1.5 1,435,335 + DVU1356 0.10 +2.2 1,435,352 + DVU1356 0.13 +0.2 1,435,352 + DVU1356 0.13 +0.1 1,435,360 - DVU1356 0.14 +0.6 1,435,360 - DVU1356 0.14 -0.9 1,435,435 - DVU1356 0.23 -0.4 1,435,525 + DVU1356 0.34 -0.8 1,435,625 - DVU1356 0.47 -0.1 1,435,716 - DVU1356 0.58 -1.4 1,435,759 - DVU1356 0.63 -0.6 1,435,810 + DVU1356 0.70 -0.4 1,435,823 + DVU1356 0.71 -0.4 1,436,036 + +0.3 1,436,087 + -1.8 1,436,120 + DVU1357 0.11 -0.2 1,436,659 + +0.2 1,436,683 + -1.0 1,436,737 - +0.4 1,436,804 - +0.4 1,436,810 + -0.0 1,436,837 - +0.1 1,436,877 - -0.7 1,436,968 + DVU1358 0.10 -1.3 1,436,976 - DVU1358 0.12 -0.0 1,437,052 - DVU1358 0.23 -0.9
Or see this region's nucleotide sequence