Experiment: MoLS4 with L-Homoserine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoU and DVU1086 are separated by 427 nucleotides DVU1086 and DVU1087 are separated by 3 nucleotides DVU1087 and DVU1088 are separated by 46 nucleotides DVU1088 and alaS are separated by 379 nucleotides
DVU1085: phoU - phosphate transport system protein PhoU (TIGR), at 1,188,620 to 1,189,285
phoU
DVU1086: DVU1086 - hypothetical protein (TIGR), at 1,189,713 to 1,190,192
DVU1086
DVU1087: DVU1087 - conserved hypothetical protein (TIGR), at 1,190,196 to 1,191,176
DVU1087
DVU1088: DVU1088 - hypothetical protein (TIGR), at 1,191,223 to 1,191,399
DVU1088
DVU1089: alaS - alanyl-tRNA synthetase (TIGR), at 1,191,779 to 1,194,418
alaS
Position (kb)
1190
1191
1192 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1189.280 kb on + strand at 1189.336 kb on - strand at 1189.390 kb on - strand at 1189.514 kb on - strand at 1189.627 kb on + strand at 1189.627 kb on + strand at 1189.653 kb on + strand at 1189.789 kb on + strand, within DVU1086 at 1189.848 kb on + strand, within DVU1086 at 1189.911 kb on + strand, within DVU1086 at 1189.963 kb on + strand, within DVU1086 at 1189.966 kb on - strand, within DVU1086 at 1190.011 kb on - strand, within DVU1086 at 1190.063 kb on - strand, within DVU1086 at 1190.084 kb on + strand, within DVU1086 at 1190.084 kb on + strand, within DVU1086 at 1190.106 kb on + strand, within DVU1086 at 1190.186 kb on + strand at 1190.215 kb on - strand at 1190.537 kb on - strand, within DVU1087 at 1190.640 kb on + strand, within DVU1087 at 1190.768 kb on - strand, within DVU1087 at 1190.773 kb on - strand, within DVU1087 at 1190.915 kb on + strand, within DVU1087 at 1190.938 kb on - strand, within DVU1087 at 1190.985 kb on - strand, within DVU1087 at 1191.028 kb on - strand, within DVU1087 at 1191.083 kb on + strand at 1191.083 kb on + strand at 1191.109 kb on + strand at 1191.112 kb on - strand at 1191.117 kb on - strand at 1191.117 kb on - strand at 1191.284 kb on + strand, within DVU1088 at 1191.402 kb on - strand at 1191.402 kb on - strand at 1191.402 kb on - strand at 1191.427 kb on + strand at 1191.435 kb on - strand at 1191.446 kb on - strand at 1191.472 kb on + strand at 1191.480 kb on - strand at 1191.514 kb on - strand at 1191.665 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Homoserine as nutrient 10mM remove 1,189,280 + +2.8 1,189,336 - +0.9 1,189,390 - -0.0 1,189,514 - -0.2 1,189,627 + +0.5 1,189,627 + -0.4 1,189,653 + +0.2 1,189,789 + DVU1086 0.16 -0.2 1,189,848 + DVU1086 0.28 +0.0 1,189,911 + DVU1086 0.41 -0.3 1,189,963 + DVU1086 0.52 -0.1 1,189,966 - DVU1086 0.53 -1.2 1,190,011 - DVU1086 0.62 +1.0 1,190,063 - DVU1086 0.73 -1.0 1,190,084 + DVU1086 0.77 -0.1 1,190,084 + DVU1086 0.77 -1.5 1,190,106 + DVU1086 0.82 +0.3 1,190,186 + +0.5 1,190,215 - -0.4 1,190,537 - DVU1087 0.35 -1.1 1,190,640 + DVU1087 0.45 -1.1 1,190,768 - DVU1087 0.58 +0.3 1,190,773 - DVU1087 0.59 -1.4 1,190,915 + DVU1087 0.73 -1.4 1,190,938 - DVU1087 0.76 -1.5 1,190,985 - DVU1087 0.80 -1.5 1,191,028 - DVU1087 0.85 -1.6 1,191,083 + -2.1 1,191,083 + -1.6 1,191,109 + -1.5 1,191,112 - -1.6 1,191,117 - -0.3 1,191,117 - -1.5 1,191,284 + DVU1088 0.34 -1.6 1,191,402 - +0.8 1,191,402 - -0.0 1,191,402 - -0.8 1,191,427 + -0.2 1,191,435 - +0.2 1,191,446 - +0.1 1,191,472 + -0.2 1,191,480 - +0.3 1,191,514 - +0.4 1,191,665 + -1.5
Or see this region's nucleotide sequence