Experiment: KB with 4-hydroxychalcone 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_RNA26 and Psyr_RNA27 are separated by 21 nucleotides Psyr_RNA27 and Psyr_1589 are separated by 360 nucleotides Psyr_1589 and Psyr_1590 are separated by 125 nucleotides Psyr_1590 and Psyr_1591 are separated by 55 nucleotides Psyr_1591 and Psyr_1592 are separated by 32 nucleotides
Psyr_RNA26: Psyr_RNA26 - tRNA-Val, at 1,788,216 to 1,788,291
_RNA26
Psyr_RNA27: Psyr_RNA27 - tRNA-Asp, at 1,788,313 to 1,788,389
_RNA27
Psyr_1589: Psyr_1589 - conserved hypothetical protein, at 1,788,750 to 1,789,145
_1589
Psyr_1590: Psyr_1590 - L-carnitine dehydratase/bile acid-inducible protein F, at 1,789,271 to 1,790,461
_1590
Psyr_1591: Psyr_1591 - Glyoxalase/bleomycin resistance protein/dioxygenase, at 1,790,517 to 1,790,909
_1591
Psyr_1592: Psyr_1592 - conserved hypothetical protein, at 1,790,942 to 1,791,460
_1592
Position (kb)
1789
1790
1791 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1788.549 kb on + strand at 1788.549 kb on + strand at 1788.586 kb on + strand at 1788.627 kb on + strand at 1788.630 kb on + strand at 1788.631 kb on - strand at 1788.631 kb on - strand at 1788.687 kb on - strand at 1788.765 kb on - strand at 1788.775 kb on + strand at 1788.775 kb on + strand at 1788.775 kb on + strand at 1788.776 kb on - strand at 1788.823 kb on + strand, within Psyr_1589 at 1788.893 kb on - strand, within Psyr_1589 at 1788.897 kb on + strand, within Psyr_1589 at 1788.916 kb on - strand, within Psyr_1589 at 1788.927 kb on + strand, within Psyr_1589 at 1788.927 kb on + strand, within Psyr_1589 at 1788.928 kb on - strand, within Psyr_1589 at 1788.928 kb on - strand, within Psyr_1589 at 1789.020 kb on + strand, within Psyr_1589 at 1789.069 kb on + strand, within Psyr_1589 at 1789.069 kb on + strand, within Psyr_1589 at 1789.079 kb on + strand, within Psyr_1589 at 1789.079 kb on + strand, within Psyr_1589 at 1789.079 kb on + strand, within Psyr_1589 at 1789.080 kb on - strand, within Psyr_1589 at 1789.080 kb on - strand, within Psyr_1589 at 1789.139 kb on + strand at 1789.139 kb on + strand at 1789.140 kb on - strand at 1789.542 kb on + strand, within Psyr_1590 at 1789.542 kb on + strand, within Psyr_1590 at 1789.542 kb on + strand, within Psyr_1590 at 1789.542 kb on + strand, within Psyr_1590 at 1789.542 kb on + strand, within Psyr_1590 at 1789.542 kb on + strand, within Psyr_1590 at 1789.542 kb on + strand, within Psyr_1590 at 1789.542 kb on + strand, within Psyr_1590 at 1789.543 kb on - strand, within Psyr_1590 at 1789.543 kb on - strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.857 kb on + strand, within Psyr_1590 at 1789.858 kb on - strand, within Psyr_1590 at 1789.858 kb on - strand, within Psyr_1590 at 1789.858 kb on - strand, within Psyr_1590 at 1789.858 kb on - strand, within Psyr_1590 at 1789.858 kb on - strand, within Psyr_1590 at 1789.858 kb on - strand, within Psyr_1590 at 1789.858 kb on - strand, within Psyr_1590 at 1789.858 kb on - strand, within Psyr_1590 at 1789.871 kb on + strand, within Psyr_1590 at 1789.871 kb on + strand, within Psyr_1590 at 1789.871 kb on + strand, within Psyr_1590 at 1789.872 kb on - strand, within Psyr_1590 at 1789.872 kb on - strand, within Psyr_1590 at 1789.872 kb on - strand, within Psyr_1590 at 1790.003 kb on + strand, within Psyr_1590 at 1790.004 kb on - strand, within Psyr_1590 at 1790.032 kb on + strand, within Psyr_1590 at 1790.033 kb on - strand, within Psyr_1590 at 1790.033 kb on - strand, within Psyr_1590 at 1790.204 kb on - strand, within Psyr_1590 at 1790.222 kb on + strand, within Psyr_1590 at 1790.255 kb on + strand, within Psyr_1590 at 1790.320 kb on + strand, within Psyr_1590 at 1790.320 kb on + strand, within Psyr_1590 at 1790.356 kb on - strand at 1790.356 kb on - strand at 1790.455 kb on + strand at 1790.496 kb on - strand at 1790.542 kb on + strand at 1790.542 kb on + strand at 1790.580 kb on + strand, within Psyr_1591 at 1790.580 kb on + strand, within Psyr_1591 at 1790.584 kb on + strand, within Psyr_1591 at 1790.584 kb on + strand, within Psyr_1591 at 1790.585 kb on - strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.844 kb on + strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.845 kb on - strand, within Psyr_1591 at 1790.907 kb on + strand at 1790.908 kb on - strand at 1790.908 kb on - strand at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.012 kb on + strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.013 kb on - strand, within Psyr_1592 at 1791.086 kb on + strand, within Psyr_1592 at 1791.086 kb on + strand, within Psyr_1592 at 1791.086 kb on + strand, within Psyr_1592 at 1791.086 kb on + strand, within Psyr_1592 at 1791.087 kb on - strand, within Psyr_1592 at 1791.087 kb on - strand, within Psyr_1592 at 1791.087 kb on - strand, within Psyr_1592 at 1791.087 kb on - strand, within Psyr_1592 at 1791.087 kb on - strand, within Psyr_1592 at 1791.087 kb on - strand, within Psyr_1592 at 1791.162 kb on + strand, within Psyr_1592 at 1791.162 kb on + strand, within Psyr_1592 at 1791.162 kb on + strand, within Psyr_1592 at 1791.163 kb on - strand, within Psyr_1592 at 1791.163 kb on - strand, within Psyr_1592 at 1791.357 kb on + strand, within Psyr_1592 at 1791.358 kb on - strand, within Psyr_1592 at 1791.461 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with 4-hydroxychalcone 10 mM remove 1,788,549 + +0.1 1,788,549 + -1.5 1,788,586 + -1.1 1,788,627 + +1.3 1,788,630 + +0.4 1,788,631 - +0.7 1,788,631 - +0.5 1,788,687 - -0.7 1,788,765 - -1.2 1,788,775 + +0.2 1,788,775 + -0.0 1,788,775 + +1.5 1,788,776 - -0.3 1,788,823 + Psyr_1589 0.18 -0.2 1,788,893 - Psyr_1589 0.36 +1.3 1,788,897 + Psyr_1589 0.37 +2.1 1,788,916 - Psyr_1589 0.42 -1.2 1,788,927 + Psyr_1589 0.45 +0.1 1,788,927 + Psyr_1589 0.45 -0.7 1,788,928 - Psyr_1589 0.45 +0.1 1,788,928 - Psyr_1589 0.45 -0.6 1,789,020 + Psyr_1589 0.68 -0.6 1,789,069 + Psyr_1589 0.81 -1.5 1,789,069 + Psyr_1589 0.81 -0.8 1,789,079 + Psyr_1589 0.83 -0.1 1,789,079 + Psyr_1589 0.83 -0.4 1,789,079 + Psyr_1589 0.83 -0.6 1,789,080 - Psyr_1589 0.83 -1.7 1,789,080 - Psyr_1589 0.83 -1.7 1,789,139 + +0.2 1,789,139 + -0.7 1,789,140 - +0.1 1,789,542 + Psyr_1590 0.23 +1.0 1,789,542 + Psyr_1590 0.23 -0.2 1,789,542 + Psyr_1590 0.23 -0.4 1,789,542 + Psyr_1590 0.23 -1.3 1,789,542 + Psyr_1590 0.23 -0.6 1,789,542 + Psyr_1590 0.23 +0.7 1,789,542 + Psyr_1590 0.23 -3.8 1,789,542 + Psyr_1590 0.23 -0.5 1,789,543 - Psyr_1590 0.23 -1.2 1,789,543 - Psyr_1590 0.23 -1.3 1,789,857 + Psyr_1590 0.49 +1.1 1,789,857 + Psyr_1590 0.49 +0.5 1,789,857 + -1.3 1,789,857 + Psyr_1590 0.49 +3.4 1,789,857 + Psyr_1590 0.49 -0.2 1,789,857 + Psyr_1590 0.49 +0.2 1,789,857 + Psyr_1590 0.49 +1.6 1,789,857 + Psyr_1590 0.49 +0.8 1,789,857 + Psyr_1590 0.49 +0.1 1,789,857 + Psyr_1590 0.49 +0.1 1,789,857 + Psyr_1590 0.49 -0.9 1,789,857 + Psyr_1590 0.49 +1.1 1,789,858 - Psyr_1590 0.49 +0.8 1,789,858 - Psyr_1590 0.49 +1.4 1,789,858 - Psyr_1590 0.49 +0.4 1,789,858 - Psyr_1590 0.49 -0.3 1,789,858 - Psyr_1590 0.49 +1.4 1,789,858 - Psyr_1590 0.49 -0.1 1,789,858 - Psyr_1590 0.49 +0.7 1,789,858 - Psyr_1590 0.49 +3.1 1,789,871 + Psyr_1590 0.50 +1.3 1,789,871 + Psyr_1590 0.50 +0.1 1,789,871 + Psyr_1590 0.50 -0.5 1,789,872 - Psyr_1590 0.50 -1.3 1,789,872 - Psyr_1590 0.50 +0.2 1,789,872 - Psyr_1590 0.50 +1.6 1,790,003 + Psyr_1590 0.61 -0.7 1,790,004 - Psyr_1590 0.62 +0.1 1,790,032 + Psyr_1590 0.64 -2.0 1,790,033 - Psyr_1590 0.64 -0.9 1,790,033 - Psyr_1590 0.64 +1.4 1,790,204 - Psyr_1590 0.78 +2.4 1,790,222 + Psyr_1590 0.80 +0.2 1,790,255 + Psyr_1590 0.83 +0.1 1,790,320 + Psyr_1590 0.88 +1.2 1,790,320 + Psyr_1590 0.88 +0.5 1,790,356 - -1.9 1,790,356 - +0.1 1,790,455 + +1.3 1,790,496 - -1.4 1,790,542 + -0.3 1,790,542 + -1.1 1,790,580 + Psyr_1591 0.16 -0.1 1,790,580 + Psyr_1591 0.16 +0.6 1,790,584 + Psyr_1591 0.17 +0.8 1,790,584 + Psyr_1591 0.17 -1.1 1,790,585 - Psyr_1591 0.17 +0.5 1,790,844 + Psyr_1591 0.83 +0.9 1,790,844 + Psyr_1591 0.83 +0.6 1,790,844 + Psyr_1591 0.83 +2.6 1,790,844 + Psyr_1591 0.83 -0.5 1,790,844 + Psyr_1591 0.83 +0.5 1,790,844 + Psyr_1591 0.83 -0.2 1,790,845 - Psyr_1591 0.83 +0.7 1,790,845 - Psyr_1591 0.83 -0.3 1,790,845 - Psyr_1591 0.83 +1.1 1,790,845 - Psyr_1591 0.83 +0.3 1,790,845 - Psyr_1591 0.83 -0.6 1,790,845 - Psyr_1591 0.83 -0.2 1,790,845 - Psyr_1591 0.83 +0.7 1,790,907 + +0.7 1,790,908 - +2.1 1,790,908 - -0.3 1,791,012 + Psyr_1592 0.13 -0.8 1,791,012 + Psyr_1592 0.13 +0.4 1,791,012 + Psyr_1592 0.13 +0.9 1,791,012 + Psyr_1592 0.13 -0.0 1,791,012 + Psyr_1592 0.13 +2.4 1,791,012 + Psyr_1592 0.13 -0.6 1,791,012 + Psyr_1592 0.13 +1.2 1,791,012 + Psyr_1592 0.13 +0.2 1,791,012 + Psyr_1592 0.13 -0.7 1,791,012 + Psyr_1592 0.13 -0.7 1,791,012 + Psyr_1592 0.13 -1.3 1,791,012 + Psyr_1592 0.13 +0.1 1,791,012 + Psyr_1592 0.13 +0.8 1,791,012 + Psyr_1592 0.13 -0.9 1,791,013 - Psyr_1592 0.14 -0.2 1,791,013 - Psyr_1592 0.14 +1.1 1,791,013 - Psyr_1592 0.14 +0.2 1,791,013 - Psyr_1592 0.14 -0.1 1,791,013 - Psyr_1592 0.14 +0.1 1,791,013 - Psyr_1592 0.14 -2.6 1,791,013 - Psyr_1592 0.14 -0.2 1,791,013 - Psyr_1592 0.14 -0.4 1,791,013 - Psyr_1592 0.14 -0.2 1,791,013 - Psyr_1592 0.14 +0.1 1,791,013 - Psyr_1592 0.14 +1.3 1,791,013 - Psyr_1592 0.14 -0.7 1,791,013 - Psyr_1592 0.14 -0.6 1,791,013 - Psyr_1592 0.14 +0.3 1,791,013 - Psyr_1592 0.14 +0.3 1,791,013 - Psyr_1592 0.14 -0.9 1,791,086 + Psyr_1592 0.28 +0.0 1,791,086 + Psyr_1592 0.28 +1.3 1,791,086 + Psyr_1592 0.28 +1.4 1,791,086 + Psyr_1592 0.28 +1.7 1,791,087 - Psyr_1592 0.28 -0.3 1,791,087 - Psyr_1592 0.28 -0.2 1,791,087 - Psyr_1592 0.28 -0.2 1,791,087 - Psyr_1592 0.28 -0.5 1,791,087 - Psyr_1592 0.28 -0.2 1,791,087 - Psyr_1592 0.28 -1.3 1,791,162 + Psyr_1592 0.42 +0.0 1,791,162 + Psyr_1592 0.42 -1.3 1,791,162 + Psyr_1592 0.42 -2.4 1,791,163 - Psyr_1592 0.43 +0.2 1,791,163 - Psyr_1592 0.43 +1.8 1,791,357 + Psyr_1592 0.80 -1.2 1,791,358 - Psyr_1592 0.80 -1.1 1,791,461 - +0.3
Or see this region's nucleotide sequence