Experiment: BHIS with Chlorhexidine diacetate salt 0.0015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1821 and BT1822 are separated by 26 nucleotides BT1822 and BT1823 are separated by 178 nucleotides BT1823 and BT1824 overlap by 17 nucleotides BT1824 and BT1825 overlap by 14 nucleotides
BT1821: BT1821 - transposase (NCBI ptt file), at 2,282,267 to 2,283,430
BT1821
BT1822: BT1822 - pyruvate dehydrogenase [cytochrome] (NCBI ptt file), at 2,283,457 to 2,284,143
BT1822
BT1823: BT1823 - conserved hypothetical protein, putative membrane protein (NCBI ptt file), at 2,284,322 to 2,285,239
BT1823
BT1824: BT1824 - conserved hypothetical protein, putative permease (NCBI ptt file), at 2,285,223 to 2,286,179
BT1824
BT1825: BT1825 - conserved hypothetical protein (NCBI ptt file), at 2,286,166 to 2,286,672
BT1825
Position (kb)
2284
2285
2286 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2283.490 kb on + strand at 2283.564 kb on - strand, within BT1822 at 2283.754 kb on + strand, within BT1822 at 2283.852 kb on + strand, within BT1822 at 2283.936 kb on + strand, within BT1822 at 2283.937 kb on - strand, within BT1822 at 2283.937 kb on - strand, within BT1822 at 2283.942 kb on - strand, within BT1822 at 2284.018 kb on - strand, within BT1822 at 2284.101 kb on + strand at 2284.128 kb on - strand at 2284.160 kb on + strand at 2284.162 kb on + strand at 2284.166 kb on + strand at 2284.256 kb on - strand at 2284.299 kb on + strand at 2284.348 kb on - strand at 2284.364 kb on + strand at 2284.365 kb on - strand at 2284.365 kb on - strand at 2284.462 kb on + strand, within BT1823 at 2284.539 kb on + strand, within BT1823 at 2284.557 kb on + strand, within BT1823 at 2284.573 kb on - strand, within BT1823 at 2284.653 kb on - strand, within BT1823 at 2284.653 kb on - strand, within BT1823 at 2284.653 kb on - strand, within BT1823 at 2284.748 kb on - strand, within BT1823 at 2284.748 kb on - strand, within BT1823 at 2284.750 kb on + strand, within BT1823 at 2284.764 kb on - strand, within BT1823 at 2284.764 kb on - strand, within BT1823 at 2284.821 kb on + strand, within BT1823 at 2284.948 kb on + strand, within BT1823 at 2284.949 kb on - strand, within BT1823 at 2285.060 kb on + strand, within BT1823 at 2285.110 kb on + strand, within BT1823 at 2285.193 kb on - strand at 2285.237 kb on + strand at 2285.357 kb on + strand, within BT1824 at 2285.369 kb on + strand, within BT1824 at 2285.370 kb on - strand, within BT1824 at 2285.378 kb on - strand, within BT1824 at 2285.448 kb on - strand, within BT1824 at 2285.515 kb on + strand, within BT1824 at 2285.516 kb on - strand, within BT1824 at 2285.574 kb on + strand, within BT1824 at 2285.649 kb on - strand, within BT1824 at 2285.651 kb on - strand, within BT1824 at 2285.690 kb on - strand, within BT1824 at 2285.700 kb on - strand, within BT1824 at 2285.730 kb on - strand, within BT1824 at 2285.818 kb on + strand, within BT1824 at 2285.911 kb on - strand, within BT1824 at 2285.955 kb on + strand, within BT1824 at 2286.004 kb on - strand, within BT1824 at 2286.178 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorhexidine diacetate salt 0.0015625 mM remove 2,283,490 + -2.6 2,283,564 - BT1822 0.16 -1.6 2,283,754 + BT1822 0.43 -1.0 2,283,852 + BT1822 0.57 +1.0 2,283,936 + BT1822 0.70 -0.4 2,283,937 - BT1822 0.70 -0.0 2,283,937 - BT1822 0.70 -0.0 2,283,942 - BT1822 0.71 +0.6 2,284,018 - BT1822 0.82 -0.8 2,284,101 + -1.2 2,284,128 - -0.5 2,284,160 + -0.6 2,284,162 + +1.6 2,284,166 + -2.6 2,284,256 - -1.2 2,284,299 + -0.9 2,284,348 - -3.3 2,284,364 + -1.0 2,284,365 - -2.0 2,284,365 - -1.0 2,284,462 + BT1823 0.15 -0.0 2,284,539 + BT1823 0.24 -1.6 2,284,557 + BT1823 0.26 -2.2 2,284,573 - BT1823 0.27 -1.0 2,284,653 - BT1823 0.36 -2.6 2,284,653 - BT1823 0.36 -2.0 2,284,653 - BT1823 0.36 -1.0 2,284,748 - BT1823 0.46 -1.0 2,284,748 - BT1823 0.46 -0.0 2,284,750 + BT1823 0.47 -1.6 2,284,764 - BT1823 0.48 -1.6 2,284,764 - BT1823 0.48 -2.8 2,284,821 + BT1823 0.54 -1.6 2,284,948 + BT1823 0.68 -1.8 2,284,949 - BT1823 0.68 -1.5 2,285,060 + BT1823 0.80 -2.3 2,285,110 + BT1823 0.86 -1.3 2,285,193 - -1.6 2,285,237 + -1.7 2,285,357 + BT1824 0.14 -1.1 2,285,369 + BT1824 0.15 -1.6 2,285,370 - BT1824 0.15 -0.3 2,285,378 - BT1824 0.16 -3.0 2,285,448 - BT1824 0.24 -0.9 2,285,515 + BT1824 0.31 -0.5 2,285,516 - BT1824 0.31 +0.1 2,285,574 + BT1824 0.37 -0.4 2,285,649 - BT1824 0.45 -1.7 2,285,651 - BT1824 0.45 -1.2 2,285,690 - BT1824 0.49 -0.0 2,285,700 - BT1824 0.50 -0.6 2,285,730 - BT1824 0.53 -0.7 2,285,818 + BT1824 0.62 -0.0 2,285,911 - BT1824 0.72 -0.0 2,285,955 + BT1824 0.76 -2.3 2,286,004 - BT1824 0.82 -1.5 2,286,178 - -0.9
Or see this region's nucleotide sequence