Experiment: MoLS4 with L-Homoserine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0761 and DVU0762 are separated by 25 nucleotides DVU0762 and gdp are separated by 97 nucleotides gdp and hup-2 are separated by 119 nucleotides hup-2 and DORF7327 are separated by 292 nucleotides DORF7327 and DVU0765 overlap by 8 nucleotides
DVU0761: DVU0761 - lipoprotein, putative (TIGR), at 852,498 to 853,088
DVU0761
DVU0762: DVU0762 - conserved hypothetical protein (TIGR), at 853,114 to 853,584
DVU0762
DVU0763: gdp - GGDEF domain protein (TIGR), at 853,682 to 854,617
gdp
DVU0764: hup-2 - DNA-binding protein HU (TIGR), at 854,737 to 855,012
hup-2
DORF7327: DORF7327 - no description, at 855,305 to 855,571
DORF7327
DVU0765: DVU0765 - hydroxypyruvate reductase, putative (TIGR), at 855,564 to 856,928
DVU0765
Position (kb) 853
854
855 Strain fitness (log2 ratio) -2
-1
0
1
2 at 852.701 kb on - strand, within DVU0761 at 852.763 kb on + strand, within DVU0761 at 852.824 kb on + strand, within DVU0761 at 852.833 kb on - strand, within DVU0761 at 852.901 kb on + strand, within DVU0761 at 852.909 kb on - strand, within DVU0761 at 852.949 kb on + strand, within DVU0761 at 852.991 kb on + strand, within DVU0761 at 853.085 kb on + strand at 853.093 kb on - strand at 853.096 kb on + strand at 853.105 kb on + strand at 853.113 kb on - strand at 853.135 kb on + strand at 853.178 kb on - strand, within DVU0762 at 853.192 kb on - strand, within DVU0762 at 853.199 kb on - strand, within DVU0762 at 853.207 kb on - strand, within DVU0762 at 853.207 kb on - strand, within DVU0762 at 853.219 kb on - strand, within DVU0762 at 853.342 kb on + strand, within DVU0762 at 853.362 kb on + strand, within DVU0762 at 853.370 kb on - strand, within DVU0762 at 853.389 kb on - strand, within DVU0762 at 853.396 kb on - strand, within DVU0762 at 853.498 kb on + strand, within DVU0762 at 853.532 kb on - strand, within DVU0762 at 853.545 kb on - strand at 853.548 kb on + strand at 853.600 kb on + strand at 853.600 kb on + strand at 853.736 kb on + strand at 853.784 kb on + strand, within gdp at 853.890 kb on + strand, within gdp at 854.058 kb on - strand, within gdp at 854.060 kb on - strand, within gdp at 854.102 kb on + strand, within gdp at 854.112 kb on - strand, within gdp at 854.128 kb on + strand, within gdp at 854.156 kb on + strand, within gdp at 854.171 kb on + strand, within gdp at 854.179 kb on - strand, within gdp at 854.222 kb on - strand, within gdp at 854.230 kb on + strand, within gdp at 854.239 kb on + strand, within gdp at 854.276 kb on + strand, within gdp at 854.360 kb on + strand, within gdp at 854.360 kb on - strand, within gdp at 854.534 kb on + strand at 854.624 kb on - strand at 854.682 kb on - strand at 854.752 kb on - strand at 854.788 kb on + strand, within hup-2 at 854.801 kb on - strand, within hup-2 at 854.885 kb on + strand, within hup-2 at 854.885 kb on + strand, within hup-2 at 855.166 kb on + strand at 855.166 kb on + strand at 855.172 kb on - strand at 855.174 kb on - strand at 855.174 kb on - strand at 855.184 kb on - strand at 855.184 kb on - strand at 855.208 kb on - strand at 855.258 kb on - strand at 855.282 kb on + strand at 855.289 kb on + strand at 855.290 kb on - strand at 855.311 kb on - strand at 855.367 kb on + strand, within DORF7327 at 855.385 kb on + strand, within DORF7327 at 855.398 kb on + strand, within DORF7327 at 855.414 kb on - strand, within DORF7327 at 855.453 kb on - strand, within DORF7327 at 855.570 kb on - strand at 855.603 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Homoserine as nutrient 10mM remove 852,701 - DVU0761 0.34 -0.3 852,763 + DVU0761 0.45 -0.1 852,824 + DVU0761 0.55 -1.0 852,833 - DVU0761 0.57 +2.5 852,901 + DVU0761 0.68 -0.6 852,909 - DVU0761 0.70 +0.1 852,949 + DVU0761 0.76 -1.4 852,991 + DVU0761 0.83 +0.3 853,085 + -0.1 853,093 - +0.3 853,096 + +1.5 853,105 + -0.2 853,113 - -0.3 853,135 + -0.2 853,178 - DVU0762 0.14 -0.3 853,192 - DVU0762 0.17 +0.3 853,199 - DVU0762 0.18 -1.4 853,207 - DVU0762 0.20 +0.2 853,207 - DVU0762 0.20 -1.2 853,219 - DVU0762 0.22 -0.1 853,342 + DVU0762 0.48 -0.8 853,362 + DVU0762 0.53 -0.0 853,370 - DVU0762 0.54 +1.2 853,389 - DVU0762 0.58 +0.0 853,396 - DVU0762 0.60 +0.1 853,498 + DVU0762 0.82 +0.7 853,532 - DVU0762 0.89 +0.6 853,545 - -0.3 853,548 + -0.4 853,600 + -0.8 853,600 + +0.0 853,736 + -0.4 853,784 + gdp DVU0763 0.11 -0.2 853,890 + gdp DVU0763 0.22 +0.1 854,058 - gdp DVU0763 0.40 -2.1 854,060 - gdp DVU0763 0.40 +1.1 854,102 + gdp DVU0763 0.45 +0.3 854,112 - gdp DVU0763 0.46 -1.2 854,128 + gdp DVU0763 0.48 +0.1 854,156 + gdp DVU0763 0.51 -0.8 854,171 + gdp DVU0763 0.52 +0.0 854,179 - gdp DVU0763 0.53 -0.1 854,222 - gdp DVU0763 0.58 +0.3 854,230 + gdp DVU0763 0.59 -0.1 854,239 + gdp DVU0763 0.60 +0.0 854,276 + gdp DVU0763 0.63 +0.2 854,360 + gdp DVU0763 0.72 -0.1 854,360 - gdp DVU0763 0.72 -0.1 854,534 + -0.7 854,624 - -1.7 854,682 - +0.9 854,752 - -1.1 854,788 + hup-2 DVU0764 0.18 -1.7 854,801 - hup-2 DVU0764 0.23 +0.5 854,885 + hup-2 DVU0764 0.54 +0.1 854,885 + hup-2 DVU0764 0.54 +0.5 855,166 + -0.5 855,166 + +0.3 855,172 - +0.0 855,174 - -1.6 855,174 - -0.3 855,184 - +0.2 855,184 - +0.7 855,208 - -0.1 855,258 - +0.1 855,282 + -0.5 855,289 + -0.1 855,290 - -0.3 855,311 - -1.4 855,367 + DORF7327 0.23 +0.5 855,385 + DORF7327 0.30 +0.3 855,398 + DORF7327 0.35 -0.1 855,414 - DORF7327 0.41 +0.8 855,453 - DORF7327 0.55 +0.1 855,570 - +0.9 855,603 - +1.3
Or see this region's nucleotide sequence