Experiment: KB with Curcumin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2002 and Psyr_2003 are separated by 74 nucleotides Psyr_2003 and Psyr_2004 are separated by 66 nucleotides Psyr_2004 and Psyr_2005 are separated by 361 nucleotides Psyr_2005 and Psyr_2006 overlap by 7 nucleotides
Psyr_2002: Psyr_2002 - Protein of unknown function UPF0153, at 2,323,529 to 2,323,786
_2002
Psyr_2003: Psyr_2003 - conserved hypothetical protein, at 2,323,861 to 2,324,454
_2003
Psyr_2004: Psyr_2004 - citrate synthase, at 2,324,521 to 2,325,810
_2004
Psyr_2005: Psyr_2005 - succinate dehydrogenase subunit C, at 2,326,172 to 2,326,546
_2005
Psyr_2006: Psyr_2006 - succinate dehydrogenase subunit D, at 2,326,540 to 2,326,908
_2006
Position (kb)
2324
2325
2326 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2323.563 kb on + strand, within Psyr_2002 at 2323.856 kb on - strand at 2323.886 kb on + strand at 2323.886 kb on + strand at 2323.887 kb on - strand at 2323.887 kb on - strand at 2323.981 kb on + strand, within Psyr_2003 at 2323.981 kb on + strand, within Psyr_2003 at 2323.982 kb on - strand, within Psyr_2003 at 2323.990 kb on + strand, within Psyr_2003 at 2323.991 kb on - strand, within Psyr_2003 at 2323.999 kb on - strand, within Psyr_2003 at 2324.021 kb on + strand, within Psyr_2003 at 2324.021 kb on + strand, within Psyr_2003 at 2324.021 kb on + strand, within Psyr_2003 at 2324.128 kb on + strand, within Psyr_2003 at 2324.128 kb on + strand, within Psyr_2003 at 2324.129 kb on - strand, within Psyr_2003 at 2324.142 kb on + strand, within Psyr_2003 at 2324.143 kb on - strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.263 kb on + strand, within Psyr_2003 at 2324.263 kb on + strand, within Psyr_2003 at 2324.264 kb on - strand, within Psyr_2003 at 2324.264 kb on - strand, within Psyr_2003 at 2324.335 kb on + strand, within Psyr_2003 at 2324.412 kb on + strand at 2324.412 kb on + strand at 2324.412 kb on + strand at 2324.413 kb on - strand at 2324.475 kb on + strand at 2324.522 kb on + strand at 2324.523 kb on - strand at 2325.825 kb on - strand at 2325.872 kb on - strand at 2325.950 kb on + strand at 2326.026 kb on + strand at 2326.026 kb on + strand at 2326.034 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Curcumin 10 mM remove 2,323,563 + Psyr_2002 0.13 -0.8 2,323,856 - -1.7 2,323,886 + +0.2 2,323,886 + +0.1 2,323,887 - +0.4 2,323,887 - +1.2 2,323,981 + Psyr_2003 0.20 +0.6 2,323,981 + Psyr_2003 0.20 -0.3 2,323,982 - Psyr_2003 0.20 +0.1 2,323,990 + Psyr_2003 0.22 -0.0 2,323,991 - Psyr_2003 0.22 +1.8 2,323,999 - Psyr_2003 0.23 +0.9 2,324,021 + Psyr_2003 0.27 -0.9 2,324,021 + Psyr_2003 0.27 -0.0 2,324,021 + Psyr_2003 0.27 +2.2 2,324,128 + Psyr_2003 0.45 +1.3 2,324,128 + Psyr_2003 0.45 -0.1 2,324,129 - Psyr_2003 0.45 -1.1 2,324,142 + Psyr_2003 0.47 -0.8 2,324,143 - Psyr_2003 0.47 -0.0 2,324,242 + Psyr_2003 0.64 +2.9 2,324,242 + Psyr_2003 0.64 +0.6 2,324,242 + Psyr_2003 0.64 -0.1 2,324,242 + Psyr_2003 0.64 -1.9 2,324,242 + Psyr_2003 0.64 -0.1 2,324,242 + Psyr_2003 0.64 +0.1 2,324,242 + Psyr_2003 0.64 -1.4 2,324,242 + Psyr_2003 0.64 -0.8 2,324,242 + Psyr_2003 0.64 +0.9 2,324,242 + Psyr_2003 0.64 +0.4 2,324,242 + Psyr_2003 0.64 +1.2 2,324,243 - Psyr_2003 0.64 +0.0 2,324,243 - Psyr_2003 0.64 -1.3 2,324,243 - Psyr_2003 0.64 +1.1 2,324,243 - Psyr_2003 0.64 -0.5 2,324,243 - Psyr_2003 0.64 -0.1 2,324,243 - Psyr_2003 0.64 -1.1 2,324,243 - Psyr_2003 0.64 +0.1 2,324,243 - Psyr_2003 0.64 +0.4 2,324,243 - Psyr_2003 0.64 +2.0 2,324,243 - Psyr_2003 0.64 +1.2 2,324,243 - Psyr_2003 0.64 -0.6 2,324,263 + Psyr_2003 0.68 -1.7 2,324,263 + Psyr_2003 0.68 -0.9 2,324,264 - Psyr_2003 0.68 +0.0 2,324,264 - Psyr_2003 0.68 +1.7 2,324,335 + Psyr_2003 0.80 -0.5 2,324,412 + +1.9 2,324,412 + -1.6 2,324,412 + +0.8 2,324,413 - -1.6 2,324,475 + +0.4 2,324,522 + -1.1 2,324,523 - -2.3 2,325,825 - -1.1 2,325,872 - +1.2 2,325,950 + +0.2 2,326,026 + +0.0 2,326,026 + +0.6 2,326,034 + +0.5
Or see this region's nucleotide sequence