Experiment: KB with Curcumin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1257 and Psyr_1258 are separated by 373 nucleotides Psyr_1258 and Psyr_1259 are separated by 7 nucleotides
Psyr_1257: Psyr_1257 - 2-isopropylmalate synthase, at 1,413,086 to 1,414,756
_1257
Psyr_1258: Psyr_1258 - Peptidase M23B, at 1,415,130 to 1,415,954
_1258
Psyr_1259: Psyr_1259 - Exodeoxyribonuclease VII large subunit, at 1,415,962 to 1,417,353
_1259
Position (kb)
1415
1416 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1414.231 kb on - strand, within Psyr_1257 at 1414.269 kb on + strand, within Psyr_1257 at 1414.269 kb on + strand, within Psyr_1257 at 1414.270 kb on - strand, within Psyr_1257 at 1414.270 kb on - strand, within Psyr_1257 at 1414.368 kb on + strand, within Psyr_1257 at 1414.368 kb on + strand, within Psyr_1257 at 1414.368 kb on + strand, within Psyr_1257 at 1414.369 kb on - strand, within Psyr_1257 at 1414.369 kb on - strand, within Psyr_1257 at 1414.369 kb on - strand, within Psyr_1257 at 1414.369 kb on - strand, within Psyr_1257 at 1414.369 kb on - strand, within Psyr_1257 at 1414.448 kb on + strand, within Psyr_1257 at 1414.691 kb on - strand at 1414.725 kb on + strand at 1414.726 kb on - strand at 1414.772 kb on + strand at 1414.772 kb on + strand at 1414.772 kb on + strand at 1414.773 kb on - strand at 1414.773 kb on - strand at 1414.811 kb on + strand at 1414.812 kb on - strand at 1414.815 kb on + strand at 1414.815 kb on + strand at 1414.816 kb on - strand at 1414.816 kb on - strand at 1414.816 kb on - strand at 1414.816 kb on - strand at 1415.024 kb on - strand at 1415.055 kb on - strand at 1415.131 kb on + strand at 1415.131 kb on + strand at 1415.132 kb on - strand at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.294 kb on + strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.295 kb on - strand, within Psyr_1258 at 1415.337 kb on + strand, within Psyr_1258 at 1415.338 kb on - strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.359 kb on + strand, within Psyr_1258 at 1415.360 kb on - strand, within Psyr_1258 at 1415.360 kb on - strand, within Psyr_1258 at 1415.360 kb on - strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.803 kb on + strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.804 kb on - strand, within Psyr_1258 at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.888 kb on + strand at 1415.889 kb on - strand at 1415.949 kb on + strand at 1415.950 kb on - strand at 1415.950 kb on - strand at 1415.950 kb on - strand at 1415.950 kb on - strand at 1416.175 kb on + strand, within Psyr_1259 at 1416.175 kb on + strand, within Psyr_1259 at 1416.175 kb on + strand, within Psyr_1259 at 1416.176 kb on - strand, within Psyr_1259 at 1416.176 kb on - strand, within Psyr_1259
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Curcumin 10 mM remove 1,414,231 - Psyr_1257 0.69 +2.1 1,414,269 + Psyr_1257 0.71 -0.8 1,414,269 + Psyr_1257 0.71 -0.8 1,414,270 - Psyr_1257 0.71 +0.4 1,414,270 - Psyr_1257 0.71 +1.0 1,414,368 + Psyr_1257 0.77 -0.7 1,414,368 + Psyr_1257 0.77 +0.7 1,414,368 + Psyr_1257 0.77 +0.4 1,414,369 - Psyr_1257 0.77 -0.0 1,414,369 - Psyr_1257 0.77 -1.5 1,414,369 - Psyr_1257 0.77 -0.2 1,414,369 - Psyr_1257 0.77 -1.0 1,414,369 - Psyr_1257 0.77 -0.0 1,414,448 + Psyr_1257 0.82 -0.2 1,414,691 - -0.2 1,414,725 + +0.2 1,414,726 - +2.2 1,414,772 + +1.5 1,414,772 + +0.3 1,414,772 + -0.5 1,414,773 - +1.4 1,414,773 - +0.6 1,414,811 + +0.5 1,414,812 - +1.7 1,414,815 + +1.6 1,414,815 + -0.3 1,414,816 - +0.6 1,414,816 - +0.8 1,414,816 - -0.2 1,414,816 - +0.4 1,415,024 - +0.3 1,415,055 - -0.0 1,415,131 + -1.2 1,415,131 + -1.3 1,415,132 - +0.1 1,415,294 + Psyr_1258 0.20 -1.0 1,415,294 + Psyr_1258 0.20 +0.9 1,415,294 + Psyr_1258 0.20 +1.1 1,415,294 + Psyr_1258 0.20 +0.1 1,415,294 + Psyr_1258 0.20 +0.6 1,415,294 + Psyr_1258 0.20 +1.2 1,415,295 - Psyr_1258 0.20 +3.0 1,415,295 - Psyr_1258 0.20 -0.0 1,415,295 - Psyr_1258 0.20 +1.0 1,415,295 - Psyr_1258 0.20 +0.3 1,415,295 - Psyr_1258 0.20 -0.5 1,415,295 - Psyr_1258 0.20 +2.0 1,415,295 - Psyr_1258 0.20 -0.3 1,415,337 + Psyr_1258 0.25 +0.1 1,415,338 - Psyr_1258 0.25 +0.8 1,415,359 + Psyr_1258 0.28 +0.6 1,415,359 + Psyr_1258 0.28 +0.2 1,415,359 + Psyr_1258 0.28 +0.2 1,415,359 + Psyr_1258 0.28 -1.3 1,415,359 + Psyr_1258 0.28 +1.0 1,415,359 + Psyr_1258 0.28 +0.4 1,415,359 + Psyr_1258 0.28 +0.5 1,415,359 + Psyr_1258 0.28 -0.2 1,415,359 + Psyr_1258 0.28 -0.3 1,415,360 - Psyr_1258 0.28 -0.3 1,415,360 - Psyr_1258 0.28 +0.2 1,415,360 - Psyr_1258 0.28 +0.1 1,415,803 + Psyr_1258 0.82 -1.3 1,415,803 + Psyr_1258 0.82 +0.9 1,415,803 + Psyr_1258 0.82 +1.3 1,415,803 + Psyr_1258 0.82 -0.2 1,415,804 - Psyr_1258 0.82 +1.0 1,415,804 - Psyr_1258 0.82 +1.1 1,415,804 - Psyr_1258 0.82 +1.0 1,415,804 - Psyr_1258 0.82 +1.2 1,415,804 - Psyr_1258 0.82 +1.3 1,415,888 + +2.6 1,415,888 + -0.6 1,415,888 + -0.0 1,415,888 + -0.4 1,415,888 + +1.2 1,415,889 - +2.0 1,415,949 + +0.8 1,415,950 - +0.2 1,415,950 - +1.0 1,415,950 - +0.6 1,415,950 - +1.0 1,416,175 + Psyr_1259 0.15 +4.9 1,416,175 + Psyr_1259 0.15 -0.5 1,416,175 + Psyr_1259 0.15 -0.6 1,416,176 - Psyr_1259 0.15 -0.2 1,416,176 - Psyr_1259 0.15 -1.8
Or see this region's nucleotide sequence