Experiment: H2 McCas with 1 mM Na2S
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07665 and MMP_RS07670 are separated by 45 nucleotides MMP_RS07670 and MMP_RS07675 are separated by 105 nucleotides MMP_RS07675 and MMP_RS07680 are separated by 99 nucleotides MMP_RS07680 and MMP_RS07685 are separated by 13 nucleotides
MMP_RS07665: MMP_RS07665 - amidohydrolase, at 1,451,888 to 1,453,156
_RS07665
MMP_RS07670: MMP_RS07670 - orotate phosphoribosyltransferase, at 1,453,202 to 1,453,759
_RS07670
MMP_RS07675: MMP_RS07675 - S-layer protein, at 1,453,865 to 1,455,208
_RS07675
MMP_RS07680: MMP_RS07680 - DUF366 family protein, at 1,455,308 to 1,455,910
_RS07680
MMP_RS07685: MMP_RS07685 - TIGR01212 family radical SAM protein, at 1,455,924 to 1,456,922
_RS07685
Position (kb)
1453
1454
1455
1456 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1453.034 kb on - strand at 1453.132 kb on - strand at 1453.132 kb on - strand at 1453.148 kb on - strand at 1453.398 kb on + strand at 1453.771 kb on - strand at 1453.795 kb on - strand at 1453.798 kb on + strand at 1453.804 kb on - strand at 1453.815 kb on - strand at 1453.815 kb on - strand at 1453.815 kb on - strand at 1453.817 kb on - strand at 1453.818 kb on + strand at 1453.820 kb on - strand at 1453.821 kb on + strand at 1453.826 kb on + strand at 1453.828 kb on - strand at 1453.828 kb on - strand at 1453.829 kb on + strand at 1453.880 kb on + strand at 1453.888 kb on + strand at 1453.893 kb on - strand at 1453.897 kb on - strand at 1453.903 kb on + strand at 1453.925 kb on + strand at 1453.925 kb on + strand at 1453.925 kb on + strand at 1453.926 kb on - strand at 1453.927 kb on + strand at 1453.932 kb on + strand at 1453.932 kb on + strand at 1453.936 kb on - strand at 1453.936 kb on - strand at 1453.936 kb on - strand at 1453.944 kb on - strand at 1453.945 kb on + strand at 1454.016 kb on + strand, within MMP_RS07675 at 1454.018 kb on - strand, within MMP_RS07675 at 1454.081 kb on - strand, within MMP_RS07675 at 1454.082 kb on + strand, within MMP_RS07675 at 1454.082 kb on + strand, within MMP_RS07675 at 1454.128 kb on - strand, within MMP_RS07675 at 1454.129 kb on + strand, within MMP_RS07675 at 1454.166 kb on - strand, within MMP_RS07675 at 1454.171 kb on - strand, within MMP_RS07675 at 1454.187 kb on + strand, within MMP_RS07675 at 1454.255 kb on + strand, within MMP_RS07675 at 1454.256 kb on - strand, within MMP_RS07675 at 1454.261 kb on - strand, within MMP_RS07675 at 1454.261 kb on - strand, within MMP_RS07675 at 1454.261 kb on - strand, within MMP_RS07675 at 1454.306 kb on + strand, within MMP_RS07675 at 1454.311 kb on - strand, within MMP_RS07675 at 1454.312 kb on + strand, within MMP_RS07675 at 1454.337 kb on - strand, within MMP_RS07675 at 1454.338 kb on + strand, within MMP_RS07675 at 1454.338 kb on + strand, within MMP_RS07675 at 1454.338 kb on + strand, within MMP_RS07675 at 1454.338 kb on + strand, within MMP_RS07675 at 1454.339 kb on - strand, within MMP_RS07675 at 1454.339 kb on - strand, within MMP_RS07675 at 1454.340 kb on + strand, within MMP_RS07675 at 1454.340 kb on + strand, within MMP_RS07675 at 1454.340 kb on + strand, within MMP_RS07675 at 1454.341 kb on - strand, within MMP_RS07675 at 1454.341 kb on - strand, within MMP_RS07675 at 1454.341 kb on - strand, within MMP_RS07675 at 1454.341 kb on - strand, within MMP_RS07675 at 1454.341 kb on - strand, within MMP_RS07675 at 1454.342 kb on + strand, within MMP_RS07675 at 1454.342 kb on + strand, within MMP_RS07675 at 1454.342 kb on + strand, within MMP_RS07675 at 1454.342 kb on + strand, within MMP_RS07675 at 1454.382 kb on - strand, within MMP_RS07675 at 1454.441 kb on + strand, within MMP_RS07675 at 1454.441 kb on + strand, within MMP_RS07675 at 1454.444 kb on - strand, within MMP_RS07675 at 1454.445 kb on + strand, within MMP_RS07675 at 1454.445 kb on + strand, within MMP_RS07675 at 1454.445 kb on + strand, within MMP_RS07675 at 1454.477 kb on - strand, within MMP_RS07675 at 1454.503 kb on - strand, within MMP_RS07675 at 1454.573 kb on + strand, within MMP_RS07675 at 1454.619 kb on + strand, within MMP_RS07675 at 1454.623 kb on - strand, within MMP_RS07675 at 1454.659 kb on + strand, within MMP_RS07675 at 1454.666 kb on + strand, within MMP_RS07675 at 1454.685 kb on - strand, within MMP_RS07675 at 1454.686 kb on + strand, within MMP_RS07675 at 1454.720 kb on + strand, within MMP_RS07675 at 1454.730 kb on - strand, within MMP_RS07675 at 1454.735 kb on - strand, within MMP_RS07675 at 1454.735 kb on - strand, within MMP_RS07675 at 1454.735 kb on - strand, within MMP_RS07675 at 1454.735 kb on - strand, within MMP_RS07675 at 1454.741 kb on - strand, within MMP_RS07675 at 1454.742 kb on + strand, within MMP_RS07675 at 1454.746 kb on - strand, within MMP_RS07675 at 1454.777 kb on + strand, within MMP_RS07675 at 1454.781 kb on - strand, within MMP_RS07675 at 1454.781 kb on - strand, within MMP_RS07675 at 1454.833 kb on + strand, within MMP_RS07675 at 1454.834 kb on - strand, within MMP_RS07675 at 1454.843 kb on + strand, within MMP_RS07675 at 1454.895 kb on - strand, within MMP_RS07675 at 1454.941 kb on + strand, within MMP_RS07675 at 1454.944 kb on + strand, within MMP_RS07675 at 1455.005 kb on + strand, within MMP_RS07675 at 1455.050 kb on + strand, within MMP_RS07675 at 1455.276 kb on + strand at 1455.344 kb on + strand at 1455.588 kb on - strand, within MMP_RS07680 at 1455.795 kb on - strand, within MMP_RS07680 at 1455.817 kb on - strand, within MMP_RS07680 at 1455.847 kb on - strand, within MMP_RS07680 at 1455.847 kb on - strand, within MMP_RS07680 at 1455.914 kb on + strand at 1455.914 kb on + strand at 1456.072 kb on + strand, within MMP_RS07685 at 1456.076 kb on + strand, within MMP_RS07685 at 1456.158 kb on - strand, within MMP_RS07685 at 1456.159 kb on + strand, within MMP_RS07685
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 1 mM Na2S remove 1,453,034 - -3.1 1,453,132 - -2.1 1,453,132 - -2.9 1,453,148 - -2.9 1,453,398 + -2.9 1,453,771 - -0.4 1,453,795 - -2.5 1,453,798 + -2.7 1,453,804 - -1.6 1,453,815 - -1.6 1,453,815 - -0.5 1,453,815 - -1.6 1,453,817 - +0.2 1,453,818 + -1.5 1,453,820 - -1.3 1,453,821 + -2.4 1,453,826 + -3.8 1,453,828 - -1.1 1,453,828 - -1.1 1,453,829 + -3.0 1,453,880 + +0.6 1,453,888 + +0.5 1,453,893 - +0.5 1,453,897 - +0.6 1,453,903 + +0.9 1,453,925 + +0.3 1,453,925 + +1.2 1,453,925 + +1.2 1,453,926 - +0.2 1,453,927 + +0.3 1,453,932 + +0.1 1,453,932 + +1.0 1,453,936 - +0.4 1,453,936 - +0.4 1,453,936 - +0.4 1,453,944 - -1.4 1,453,945 + -0.2 1,454,016 + MMP_RS07675 0.11 +0.7 1,454,018 - MMP_RS07675 0.11 +0.9 1,454,081 - MMP_RS07675 0.16 +0.9 1,454,082 + MMP_RS07675 0.16 +0.5 1,454,082 + MMP_RS07675 0.16 +0.2 1,454,128 - MMP_RS07675 0.20 +0.1 1,454,129 + MMP_RS07675 0.20 +0.6 1,454,166 - MMP_RS07675 0.22 +0.5 1,454,171 - MMP_RS07675 0.23 +1.2 1,454,187 + MMP_RS07675 0.24 +0.3 1,454,255 + MMP_RS07675 0.29 +0.1 1,454,256 - MMP_RS07675 0.29 +0.2 1,454,261 - MMP_RS07675 0.29 +0.3 1,454,261 - MMP_RS07675 0.29 -0.4 1,454,261 - MMP_RS07675 0.29 +0.1 1,454,306 + MMP_RS07675 0.33 +1.1 1,454,311 - MMP_RS07675 0.33 -0.5 1,454,312 + MMP_RS07675 0.33 +0.7 1,454,337 - MMP_RS07675 0.35 +1.2 1,454,338 + MMP_RS07675 0.35 +0.5 1,454,338 + MMP_RS07675 0.35 +0.9 1,454,338 + MMP_RS07675 0.35 +0.6 1,454,338 + MMP_RS07675 0.35 +1.2 1,454,339 - MMP_RS07675 0.35 +0.0 1,454,339 - MMP_RS07675 0.35 +1.0 1,454,340 + MMP_RS07675 0.35 +0.0 1,454,340 + MMP_RS07675 0.35 -0.1 1,454,340 + MMP_RS07675 0.35 +0.1 1,454,341 - MMP_RS07675 0.35 -0.9 1,454,341 - MMP_RS07675 0.35 +0.8 1,454,341 - MMP_RS07675 0.35 +0.3 1,454,341 - MMP_RS07675 0.35 +0.6 1,454,341 - MMP_RS07675 0.35 +0.4 1,454,342 + MMP_RS07675 0.35 +1.2 1,454,342 + MMP_RS07675 0.35 +0.6 1,454,342 + MMP_RS07675 0.35 -1.8 1,454,342 + MMP_RS07675 0.35 -0.2 1,454,382 - MMP_RS07675 0.38 +0.9 1,454,441 + MMP_RS07675 0.43 +0.8 1,454,441 + MMP_RS07675 0.43 -0.7 1,454,444 - MMP_RS07675 0.43 +0.2 1,454,445 + MMP_RS07675 0.43 +0.7 1,454,445 + MMP_RS07675 0.43 +1.0 1,454,445 + MMP_RS07675 0.43 +0.7 1,454,477 - MMP_RS07675 0.46 +0.2 1,454,503 - MMP_RS07675 0.47 +0.2 1,454,573 + MMP_RS07675 0.53 +0.3 1,454,619 + MMP_RS07675 0.56 -0.2 1,454,623 - MMP_RS07675 0.56 +0.3 1,454,659 + MMP_RS07675 0.59 -0.2 1,454,666 + MMP_RS07675 0.60 +0.9 1,454,685 - MMP_RS07675 0.61 +0.1 1,454,686 + MMP_RS07675 0.61 +0.3 1,454,720 + MMP_RS07675 0.64 +0.6 1,454,730 - MMP_RS07675 0.64 +0.3 1,454,735 - MMP_RS07675 0.65 +0.3 1,454,735 - MMP_RS07675 0.65 +1.0 1,454,735 - MMP_RS07675 0.65 +0.3 1,454,735 - MMP_RS07675 0.65 -0.7 1,454,741 - MMP_RS07675 0.65 +0.1 1,454,742 + MMP_RS07675 0.65 -0.4 1,454,746 - MMP_RS07675 0.66 +0.6 1,454,777 + MMP_RS07675 0.68 +0.5 1,454,781 - MMP_RS07675 0.68 -1.4 1,454,781 - MMP_RS07675 0.68 -2.5 1,454,833 + MMP_RS07675 0.72 +0.8 1,454,834 - MMP_RS07675 0.72 +0.1 1,454,843 + MMP_RS07675 0.73 +0.9 1,454,895 - MMP_RS07675 0.77 +0.6 1,454,941 + MMP_RS07675 0.80 +0.9 1,454,944 + MMP_RS07675 0.80 +0.2 1,455,005 + MMP_RS07675 0.85 -0.3 1,455,050 + MMP_RS07675 0.88 +0.3 1,455,276 + +0.5 1,455,344 + -0.4 1,455,588 - MMP_RS07680 0.46 +1.0 1,455,795 - MMP_RS07680 0.81 +0.7 1,455,817 - MMP_RS07680 0.84 +0.4 1,455,847 - MMP_RS07680 0.89 +0.8 1,455,847 - MMP_RS07680 0.89 -0.4 1,455,914 + -0.6 1,455,914 + -0.2 1,456,072 + MMP_RS07685 0.15 -0.4 1,456,076 + MMP_RS07685 0.15 +0.3 1,456,158 - MMP_RS07685 0.23 -0.6 1,456,159 + MMP_RS07685 0.24 -0.3
Or see this region's nucleotide sequence