Experiment: H2 McCas with 1 mM Na2S
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04505 and MMP_RS04510 are separated by 15 nucleotides MMP_RS04510 and MMP_RS04515 are separated by 13 nucleotides MMP_RS04515 and MMP_RS04520 are separated by 443 nucleotides MMP_RS04520 and MMP_RS04525 are separated by 31 nucleotides
MMP_RS04505: MMP_RS04505 - glycine betaine ABC transporter substrate-binding protein, at 857,796 to 858,683
_RS04505
MMP_RS04510: MMP_RS04510 - ABC transporter permease subunit, at 858,699 to 859,532
_RS04510
MMP_RS04515: MMP_RS04515 - betaine/proline/choline family ABC transporter ATP-binding protein, at 859,546 to 860,715
_RS04515
MMP_RS04520: MMP_RS04520 - nucleoside deaminase, at 861,159 to 861,596
_RS04520
MMP_RS04525: MMP_RS04525 - D-2-hydroxyacid dehydrogenase, at 861,628 to 862,584
_RS04525
Position (kb) 859
860
861 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 858.546 kb on + strand, within MMP_RS04505 at 858.546 kb on + strand, within MMP_RS04505 at 858.697 kb on - strand at 858.759 kb on + strand at 858.794 kb on - strand, within MMP_RS04510 at 858.795 kb on + strand, within MMP_RS04510 at 858.816 kb on - strand, within MMP_RS04510 at 858.946 kb on + strand, within MMP_RS04510 at 859.115 kb on + strand, within MMP_RS04510 at 859.115 kb on + strand, within MMP_RS04510 at 859.141 kb on + strand, within MMP_RS04510 at 859.170 kb on - strand, within MMP_RS04510 at 859.171 kb on + strand, within MMP_RS04510 at 859.191 kb on - strand, within MMP_RS04510 at 859.292 kb on - strand, within MMP_RS04510 at 859.295 kb on - strand, within MMP_RS04510 at 859.353 kb on - strand, within MMP_RS04510 at 859.354 kb on + strand, within MMP_RS04510 at 859.386 kb on - strand, within MMP_RS04510 at 859.431 kb on - strand, within MMP_RS04510 at 859.431 kb on - strand, within MMP_RS04510 at 859.431 kb on - strand, within MMP_RS04510 at 859.432 kb on + strand, within MMP_RS04510 at 859.435 kb on - strand, within MMP_RS04510 at 859.436 kb on + strand, within MMP_RS04510 at 859.467 kb on + strand at 859.467 kb on + strand at 859.517 kb on - strand at 859.548 kb on + strand at 859.548 kb on + strand at 859.557 kb on - strand at 859.563 kb on - strand at 859.587 kb on + strand at 859.613 kb on + strand at 859.673 kb on + strand, within MMP_RS04515 at 859.689 kb on - strand, within MMP_RS04515 at 859.690 kb on + strand, within MMP_RS04515 at 859.690 kb on + strand, within MMP_RS04515 at 859.758 kb on + strand, within MMP_RS04515 at 859.777 kb on - strand, within MMP_RS04515 at 859.777 kb on - strand, within MMP_RS04515 at 859.778 kb on + strand, within MMP_RS04515 at 859.781 kb on - strand, within MMP_RS04515 at 859.782 kb on + strand, within MMP_RS04515 at 859.799 kb on + strand, within MMP_RS04515 at 859.839 kb on - strand, within MMP_RS04515 at 859.839 kb on - strand, within MMP_RS04515 at 859.842 kb on - strand, within MMP_RS04515 at 859.843 kb on + strand, within MMP_RS04515 at 860.047 kb on - strand, within MMP_RS04515 at 860.153 kb on + strand, within MMP_RS04515 at 860.167 kb on - strand, within MMP_RS04515 at 860.391 kb on + strand, within MMP_RS04515 at 860.401 kb on + strand, within MMP_RS04515 at 860.491 kb on + strand, within MMP_RS04515 at 860.493 kb on - strand, within MMP_RS04515 at 860.493 kb on - strand, within MMP_RS04515 at 860.493 kb on - strand, within MMP_RS04515 at 860.493 kb on - strand, within MMP_RS04515 at 860.493 kb on - strand, within MMP_RS04515 at 860.494 kb on + strand, within MMP_RS04515 at 860.501 kb on + strand, within MMP_RS04515 at 860.571 kb on - strand, within MMP_RS04515 at 860.574 kb on - strand, within MMP_RS04515 at 860.575 kb on + strand, within MMP_RS04515 at 860.580 kb on - strand, within MMP_RS04515 at 860.703 kb on - strand at 860.704 kb on + strand at 860.716 kb on + strand at 860.717 kb on - strand at 860.766 kb on - strand at 860.795 kb on - strand at 860.796 kb on + strand at 860.819 kb on + strand at 860.837 kb on + strand at 860.837 kb on + strand at 860.848 kb on - strand at 860.855 kb on - strand at 860.856 kb on + strand at 860.875 kb on - strand at 860.880 kb on - strand at 860.881 kb on + strand at 860.885 kb on + strand at 860.943 kb on + strand at 861.008 kb on + strand at 861.088 kb on - strand at 861.088 kb on - strand at 861.088 kb on - strand at 861.397 kb on - strand, within MMP_RS04520 at 861.423 kb on - strand, within MMP_RS04520 at 861.514 kb on - strand, within MMP_RS04520 at 861.599 kb on - strand at 861.642 kb on + strand at 861.715 kb on - strand at 861.715 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 1 mM Na2S remove 858,546 + MMP_RS04505 0.84 -0.2 858,546 + MMP_RS04505 0.84 -1.0 858,697 - +0.4 858,759 + +0.4 858,794 - MMP_RS04510 0.11 -0.3 858,795 + MMP_RS04510 0.12 -0.1 858,816 - MMP_RS04510 0.14 -0.4 858,946 + MMP_RS04510 0.30 -0.1 859,115 + MMP_RS04510 0.50 -0.3 859,115 + MMP_RS04510 0.50 -0.2 859,141 + MMP_RS04510 0.53 -0.2 859,170 - MMP_RS04510 0.56 -1.3 859,171 + MMP_RS04510 0.57 -0.3 859,191 - MMP_RS04510 0.59 +0.3 859,292 - MMP_RS04510 0.71 +0.1 859,295 - MMP_RS04510 0.71 +0.6 859,353 - MMP_RS04510 0.78 +1.0 859,354 + MMP_RS04510 0.79 -0.1 859,386 - MMP_RS04510 0.82 +0.1 859,431 - MMP_RS04510 0.88 +0.4 859,431 - MMP_RS04510 0.88 -0.2 859,431 - MMP_RS04510 0.88 -0.4 859,432 + MMP_RS04510 0.88 +1.0 859,435 - MMP_RS04510 0.88 +1.1 859,436 + MMP_RS04510 0.88 +0.0 859,467 + -0.1 859,467 + -0.4 859,517 - -1.0 859,548 + +0.3 859,548 + +0.5 859,557 - -1.2 859,563 - +0.6 859,587 + +0.7 859,613 + -1.3 859,673 + MMP_RS04515 0.11 +0.6 859,689 - MMP_RS04515 0.12 +0.2 859,690 + MMP_RS04515 0.12 +0.3 859,690 + MMP_RS04515 0.12 +0.5 859,758 + MMP_RS04515 0.18 +0.9 859,777 - MMP_RS04515 0.20 -1.7 859,777 - MMP_RS04515 0.20 +0.7 859,778 + MMP_RS04515 0.20 -1.2 859,781 - MMP_RS04515 0.20 +1.4 859,782 + MMP_RS04515 0.20 +2.0 859,799 + MMP_RS04515 0.22 +0.4 859,839 - MMP_RS04515 0.25 +0.9 859,839 - MMP_RS04515 0.25 +0.3 859,842 - MMP_RS04515 0.25 +0.2 859,843 + MMP_RS04515 0.25 +1.1 860,047 - MMP_RS04515 0.43 +1.0 860,153 + MMP_RS04515 0.52 +0.0 860,167 - MMP_RS04515 0.53 +0.4 860,391 + MMP_RS04515 0.72 +0.7 860,401 + MMP_RS04515 0.73 -0.1 860,491 + MMP_RS04515 0.81 +0.7 860,493 - MMP_RS04515 0.81 +0.4 860,493 - MMP_RS04515 0.81 +0.2 860,493 - MMP_RS04515 0.81 +0.5 860,493 - MMP_RS04515 0.81 -0.1 860,493 - MMP_RS04515 0.81 +0.3 860,494 + MMP_RS04515 0.81 -0.0 860,501 + MMP_RS04515 0.82 +0.4 860,571 - MMP_RS04515 0.88 -0.7 860,574 - MMP_RS04515 0.88 +1.0 860,575 + MMP_RS04515 0.88 +0.1 860,580 - MMP_RS04515 0.88 +0.5 860,703 - -0.6 860,704 + +0.1 860,716 + +0.0 860,717 - +0.6 860,766 - +0.1 860,795 - -0.2 860,796 + -2.3 860,819 + +0.7 860,837 + +0.2 860,837 + +0.6 860,848 - -0.6 860,855 - -0.4 860,856 + -0.4 860,875 - -0.1 860,880 - +0.9 860,881 + +0.6 860,885 + +0.5 860,943 + +0.5 861,008 + +0.5 861,088 - -1.3 861,088 - -0.1 861,088 - -0.4 861,397 - MMP_RS04520 0.54 +0.7 861,423 - MMP_RS04520 0.60 +0.2 861,514 - MMP_RS04520 0.81 -0.3 861,599 - +0.0 861,642 + +0.6 861,715 - -5.0 861,715 - +0.3
Or see this region's nucleotide sequence