Experiment: BHIS with Antimycin A 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4385 and BT4386 are separated by 41 nucleotides BT4386 and BT4387 are separated by 33 nucleotides BT4387 and BT4388 are separated by 12 nucleotides
BT4385: BT4385 - di-tripeptide ABC transporter (NCBI ptt file), at 5,778,289 to 5,779,833
BT4385
BT4386: BT4386 - hypoxanthine-guanine phosphoribosyltransferase (NCBI ptt file), at 5,779,875 to 5,780,411
BT4386
BT4387: BT4387 - adenylate kinase (ATP-AMP transphosphatase) (NCBI ptt file), at 5,780,445 to 5,781,014
BT4387
BT4388: BT4388 - GTP-binding protein (NCBI ptt file), at 5,781,027 to 5,782,193
BT4388
Position (kb) 5779
5780
5781 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5778.889 kb on - strand, within BT4385 at 5778.999 kb on + strand, within BT4385 at 5779.014 kb on + strand, within BT4385 at 5779.063 kb on + strand, within BT4385 at 5779.064 kb on - strand, within BT4385 at 5779.164 kb on + strand, within BT4385 at 5779.171 kb on - strand, within BT4385 at 5779.179 kb on - strand, within BT4385 at 5779.184 kb on - strand, within BT4385 at 5779.250 kb on + strand, within BT4385 at 5779.250 kb on + strand, within BT4385 at 5779.350 kb on + strand, within BT4385 at 5779.353 kb on + strand, within BT4385 at 5779.362 kb on + strand, within BT4385 at 5779.362 kb on + strand, within BT4385 at 5779.479 kb on + strand, within BT4385 at 5779.491 kb on - strand, within BT4385 at 5779.491 kb on - strand, within BT4385 at 5779.572 kb on + strand, within BT4385 at 5779.579 kb on - strand, within BT4385 at 5779.582 kb on - strand, within BT4385 at 5779.627 kb on + strand, within BT4385 at 5780.051 kb on + strand, within BT4386 at 5780.188 kb on + strand, within BT4386 at 5781.001 kb on + strand at 5781.012 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Antimycin A 0.1 mM remove 5,778,889 - BT4385 0.39 -2.5 5,778,999 + BT4385 0.46 +0.1 5,779,014 + BT4385 0.47 +0.1 5,779,063 + BT4385 0.50 -1.5 5,779,064 - BT4385 0.50 -0.9 5,779,164 + BT4385 0.57 -0.9 5,779,171 - BT4385 0.57 -1.3 5,779,179 - BT4385 0.58 -1.3 5,779,184 - BT4385 0.58 +2.1 5,779,250 + BT4385 0.62 -1.8 5,779,250 + BT4385 0.62 -2.5 5,779,350 + BT4385 0.69 -0.5 5,779,353 + BT4385 0.69 -1.9 5,779,362 + BT4385 0.69 -2.1 5,779,362 + BT4385 0.69 -0.1 5,779,479 + BT4385 0.77 -0.5 5,779,491 - BT4385 0.78 -0.3 5,779,491 - BT4385 0.78 -1.5 5,779,572 + BT4385 0.83 -0.9 5,779,579 - BT4385 0.83 -0.3 5,779,582 - BT4385 0.84 +0.1 5,779,627 + BT4385 0.87 +0.1 5,780,051 + BT4386 0.33 -2.9 5,780,188 + BT4386 0.58 -3.7 5,781,001 + +0.1 5,781,012 + -0.1
Or see this region's nucleotide sequence