Experiment: BHIS with Antimycin A 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1366 and BT1367 are separated by 83 nucleotides BT1367 and BT1368 overlap by 4 nucleotides
BT1366: BT1366 - hypothetical protein (NCBI ptt file), at 1,694,426 to 1,696,003
BT1366
BT1367: BT1367 - hydrolase, metal-dependent (NCBI ptt file), at 1,696,087 to 1,696,845
BT1367
BT1368: BT1368 - UDP-N-acetylenolpyruvoylglucosamine reductase (NCBI ptt file), at 1,696,842 to 1,697,837
BT1368
Position (kb)
1696
1697 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1695.104 kb on - strand, within BT1366 at 1695.241 kb on + strand, within BT1366 at 1695.428 kb on + strand, within BT1366 at 1695.429 kb on - strand, within BT1366 at 1695.429 kb on - strand, within BT1366 at 1695.429 kb on - strand, within BT1366 at 1695.429 kb on - strand, within BT1366 at 1695.429 kb on - strand, within BT1366 at 1695.440 kb on + strand, within BT1366 at 1695.523 kb on + strand, within BT1366 at 1695.523 kb on + strand, within BT1366 at 1695.524 kb on - strand, within BT1366 at 1695.529 kb on + strand, within BT1366 at 1695.542 kb on - strand, within BT1366 at 1695.580 kb on - strand, within BT1366 at 1695.778 kb on - strand, within BT1366 at 1695.877 kb on + strand at 1695.878 kb on - strand at 1695.879 kb on + strand at 1695.880 kb on - strand at 1695.880 kb on - strand at 1695.931 kb on - strand at 1695.992 kb on - strand at 1696.117 kb on - strand at 1696.117 kb on - strand at 1696.148 kb on - strand at 1696.151 kb on + strand at 1696.167 kb on + strand, within BT1367 at 1696.173 kb on - strand, within BT1367 at 1696.191 kb on - strand, within BT1367 at 1696.191 kb on - strand, within BT1367 at 1696.294 kb on + strand, within BT1367 at 1696.295 kb on - strand, within BT1367 at 1696.509 kb on - strand, within BT1367 at 1696.541 kb on + strand, within BT1367 at 1696.557 kb on + strand, within BT1367 at 1696.616 kb on + strand, within BT1367 at 1696.646 kb on - strand, within BT1367 at 1696.699 kb on + strand, within BT1367 at 1696.752 kb on + strand, within BT1367 at 1696.789 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Antimycin A 0.1 mM remove 1,695,104 - BT1366 0.43 -2.4 1,695,241 + BT1366 0.52 -0.8 1,695,428 + BT1366 0.63 +0.2 1,695,429 - BT1366 0.64 -2.1 1,695,429 - BT1366 0.64 -2.4 1,695,429 - BT1366 0.64 -1.8 1,695,429 - BT1366 0.64 -0.9 1,695,429 - BT1366 0.64 -0.8 1,695,440 + BT1366 0.64 -1.8 1,695,523 + BT1366 0.70 +1.8 1,695,523 + BT1366 0.70 -1.8 1,695,524 - BT1366 0.70 +0.2 1,695,529 + BT1366 0.70 -1.8 1,695,542 - BT1366 0.71 -1.4 1,695,580 - BT1366 0.73 -0.4 1,695,778 - BT1366 0.86 -1.0 1,695,877 + -1.4 1,695,878 - -1.4 1,695,879 + +0.2 1,695,880 - +0.2 1,695,880 - +0.6 1,695,931 - -1.9 1,695,992 - -1.6 1,696,117 - -2.4 1,696,117 - -1.8 1,696,148 - -2.1 1,696,151 + -0.4 1,696,167 + BT1367 0.11 +0.2 1,696,173 - BT1367 0.11 -2.6 1,696,191 - BT1367 0.14 -2.1 1,696,191 - BT1367 0.14 -1.4 1,696,294 + BT1367 0.27 +0.2 1,696,295 - BT1367 0.27 -2.1 1,696,509 - BT1367 0.56 -4.0 1,696,541 + BT1367 0.60 +0.2 1,696,557 + BT1367 0.62 +1.2 1,696,616 + BT1367 0.70 -2.4 1,696,646 - BT1367 0.74 +0.2 1,696,699 + BT1367 0.81 -3.1 1,696,752 + BT1367 0.88 -3.3 1,696,789 - -3.3
Or see this region's nucleotide sequence