Experiment: H2 McCas with 10 mM Na2S
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06980 and MMP_RS06985 are separated by 138 nucleotides MMP_RS06985 and MMP_RS06990 are separated by 15 nucleotides MMP_RS06990 and MMP_RS06995 are separated by 17 nucleotides
MMP_RS06980: MMP_RS06980 - tRNA 2-thiocytidine biosynthesis TtcA family protein, at 1,332,600 to 1,333,574
_RS06980
MMP_RS06985: MMP_RS06985 - tRNA 4-thiouridine(8) synthase ThiI, at 1,333,713 to 1,334,864
_RS06985
MMP_RS06990: MMP_RS06990 - CatB-related O-acetyltransferase, at 1,334,880 to 1,335,560
_RS06990
MMP_RS06995: MMP_RS06995 - TIGR00269 family protein, at 1,335,578 to 1,336,513
_RS06995
Position (kb)
1333
1334
1335 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1332.725 kb on - strand, within MMP_RS06980 at 1332.726 kb on + strand, within MMP_RS06980 at 1332.761 kb on + strand, within MMP_RS06980 at 1332.873 kb on - strand, within MMP_RS06980 at 1332.874 kb on + strand, within MMP_RS06980 at 1332.916 kb on + strand, within MMP_RS06980 at 1332.919 kb on + strand, within MMP_RS06980 at 1332.920 kb on - strand, within MMP_RS06980 at 1332.934 kb on + strand, within MMP_RS06980 at 1332.947 kb on - strand, within MMP_RS06980 at 1332.947 kb on - strand, within MMP_RS06980 at 1333.237 kb on + strand, within MMP_RS06980 at 1333.362 kb on - strand, within MMP_RS06980 at 1333.506 kb on + strand at 1333.528 kb on + strand at 1333.532 kb on + strand at 1333.563 kb on - strand at 1333.564 kb on + strand at 1333.590 kb on - strand at 1333.602 kb on + strand at 1333.602 kb on + strand at 1333.602 kb on + strand at 1333.602 kb on + strand at 1333.605 kb on - strand at 1333.605 kb on - strand at 1333.606 kb on + strand at 1333.606 kb on + strand at 1333.606 kb on + strand at 1333.608 kb on + strand at 1333.615 kb on - strand at 1333.615 kb on - strand at 1333.615 kb on - strand at 1333.617 kb on - strand at 1333.618 kb on + strand at 1333.619 kb on - strand at 1333.619 kb on - strand at 1333.620 kb on + strand at 1333.620 kb on + strand at 1333.627 kb on + strand at 1333.645 kb on + strand at 1333.649 kb on + strand at 1333.655 kb on - strand at 1333.656 kb on + strand at 1333.656 kb on + strand at 1333.660 kb on + strand at 1333.660 kb on + strand at 1333.668 kb on - strand at 1333.679 kb on + strand at 1333.679 kb on + strand at 1333.948 kb on + strand at 1334.046 kb on + strand at 1334.330 kb on - strand at 1334.345 kb on + strand at 1334.434 kb on + strand at 1334.434 kb on + strand at 1334.445 kb on + strand at 1334.750 kb on + strand at 1334.844 kb on + strand at 1334.868 kb on + strand at 1334.877 kb on + strand at 1335.025 kb on + strand, within MMP_RS06990 at 1335.053 kb on - strand, within MMP_RS06990 at 1335.061 kb on + strand, within MMP_RS06990 at 1335.095 kb on - strand, within MMP_RS06990 at 1335.104 kb on - strand, within MMP_RS06990 at 1335.144 kb on + strand, within MMP_RS06990 at 1335.271 kb on - strand, within MMP_RS06990 at 1335.286 kb on - strand, within MMP_RS06990 at 1335.287 kb on + strand, within MMP_RS06990 at 1335.360 kb on + strand, within MMP_RS06990 at 1335.377 kb on + strand, within MMP_RS06990 at 1335.503 kb on - strand at 1335.504 kb on + strand at 1335.510 kb on + strand at 1335.745 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 10 mM Na2S remove 1,332,725 - MMP_RS06980 0.13 -1.8 1,332,726 + MMP_RS06980 0.13 -2.1 1,332,761 + MMP_RS06980 0.17 -3.0 1,332,873 - MMP_RS06980 0.28 -5.7 1,332,874 + MMP_RS06980 0.28 -2.2 1,332,916 + MMP_RS06980 0.32 -2.1 1,332,919 + MMP_RS06980 0.33 -2.9 1,332,920 - MMP_RS06980 0.33 -2.3 1,332,934 + MMP_RS06980 0.34 -4.2 1,332,947 - MMP_RS06980 0.36 -2.9 1,332,947 - MMP_RS06980 0.36 -3.0 1,333,237 + MMP_RS06980 0.65 -2.3 1,333,362 - MMP_RS06980 0.78 -3.7 1,333,506 + -3.3 1,333,528 + -2.0 1,333,532 + -5.8 1,333,563 - -2.2 1,333,564 + -2.8 1,333,590 - -0.5 1,333,602 + -0.2 1,333,602 + -0.6 1,333,602 + -0.6 1,333,602 + -0.1 1,333,605 - +0.4 1,333,605 - +0.5 1,333,606 + +0.1 1,333,606 + -0.9 1,333,606 + -0.6 1,333,608 + +0.3 1,333,615 - +0.2 1,333,615 - +1.0 1,333,615 - +0.2 1,333,617 - +0.6 1,333,618 + +0.8 1,333,619 - +1.5 1,333,619 - +0.7 1,333,620 + +0.2 1,333,620 + +0.7 1,333,627 + -0.6 1,333,645 + -1.4 1,333,649 + +1.0 1,333,655 - -3.2 1,333,656 + +0.1 1,333,656 + +0.4 1,333,660 + -0.0 1,333,660 + -0.2 1,333,668 - -2.2 1,333,679 + -1.3 1,333,679 + -1.3 1,333,948 + -1.7 1,334,046 + -1.8 1,334,330 - -2.0 1,334,345 + -1.0 1,334,434 + -1.9 1,334,434 + -2.4 1,334,445 + -2.1 1,334,750 + -3.1 1,334,844 + +0.9 1,334,868 + +0.3 1,334,877 + +0.8 1,335,025 + MMP_RS06990 0.21 +0.9 1,335,053 - MMP_RS06990 0.25 +0.4 1,335,061 + MMP_RS06990 0.27 +1.2 1,335,095 - MMP_RS06990 0.32 -0.4 1,335,104 - MMP_RS06990 0.33 +0.5 1,335,144 + MMP_RS06990 0.39 -0.3 1,335,271 - MMP_RS06990 0.57 +0.8 1,335,286 - MMP_RS06990 0.60 +1.1 1,335,287 + MMP_RS06990 0.60 +0.1 1,335,360 + MMP_RS06990 0.70 +1.2 1,335,377 + MMP_RS06990 0.73 +0.6 1,335,503 - -1.3 1,335,504 + +0.3 1,335,510 + +0.6 1,335,745 + -3.3
Or see this region's nucleotide sequence