Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4488 and BT4489 are separated by 385 nucleotides BT4489 and BT4490 are separated by 37 nucleotides BT4490 and BT4491 are separated by 15 nucleotides
BT4488: BT4488 - formamidopyrimidine-DNA glycosylase (NCBI ptt file), at 5,904,430 to 5,905,269
BT4488
BT4489: BT4489 - hypothetical protein (NCBI ptt file), at 5,905,655 to 5,907,253
BT4489
BT4490: BT4490 - hypothetical protein (NCBI ptt file), at 5,907,291 to 5,907,863
BT4490
BT4491: BT4491 - hypothetical protein (NCBI ptt file), at 5,907,879 to 5,908,265
BT4491
Position (kb)
5905
5906
5907
5908 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5904.667 kb on + strand, within BT4488 at 5904.707 kb on + strand, within BT4488 at 5904.708 kb on - strand, within BT4488 at 5904.721 kb on + strand, within BT4488 at 5904.721 kb on + strand, within BT4488 at 5904.722 kb on - strand, within BT4488 at 5904.822 kb on - strand, within BT4488 at 5904.914 kb on + strand, within BT4488 at 5904.978 kb on + strand, within BT4488 at 5904.979 kb on - strand, within BT4488 at 5905.033 kb on + strand, within BT4488 at 5905.071 kb on + strand, within BT4488 at 5905.214 kb on - strand at 5905.264 kb on + strand at 5905.265 kb on - strand at 5905.265 kb on - strand at 5905.265 kb on - strand at 5905.310 kb on + strand at 5905.310 kb on + strand at 5905.310 kb on + strand at 5905.315 kb on + strand at 5905.380 kb on + strand at 5905.431 kb on + strand at 5905.431 kb on + strand at 5905.433 kb on + strand at 5905.477 kb on - strand at 5905.483 kb on - strand at 5905.591 kb on + strand at 5905.591 kb on + strand at 5905.592 kb on - strand at 5905.603 kb on - strand at 5905.633 kb on - strand at 5905.634 kb on + strand at 5905.636 kb on + strand at 5905.643 kb on + strand at 5905.644 kb on - strand at 5905.644 kb on - strand at 5905.647 kb on + strand at 5905.656 kb on + strand at 5905.669 kb on + strand at 5905.670 kb on - strand at 5905.687 kb on + strand at 5905.727 kb on - strand at 5905.739 kb on - strand at 5905.765 kb on - strand at 5905.765 kb on - strand at 5905.807 kb on + strand at 5905.808 kb on - strand at 5905.808 kb on - strand at 5905.822 kb on + strand, within BT4489 at 5905.823 kb on - strand, within BT4489 at 5905.828 kb on + strand, within BT4489 at 5905.829 kb on - strand, within BT4489 at 5905.829 kb on - strand, within BT4489 at 5905.863 kb on - strand, within BT4489 at 5905.874 kb on + strand, within BT4489 at 5905.886 kb on - strand, within BT4489 at 5905.889 kb on + strand, within BT4489 at 5905.889 kb on + strand, within BT4489 at 5905.889 kb on + strand, within BT4489 at 5905.950 kb on - strand, within BT4489 at 5905.992 kb on + strand, within BT4489 at 5906.033 kb on + strand, within BT4489 at 5906.033 kb on + strand, within BT4489 at 5906.111 kb on + strand, within BT4489 at 5906.111 kb on + strand, within BT4489 at 5906.137 kb on + strand, within BT4489 at 5906.169 kb on - strand, within BT4489 at 5906.401 kb on + strand, within BT4489 at 5906.456 kb on - strand, within BT4489 at 5906.479 kb on - strand, within BT4489 at 5906.536 kb on + strand, within BT4489 at 5906.536 kb on + strand, within BT4489 at 5906.537 kb on - strand, within BT4489 at 5906.537 kb on - strand, within BT4489 at 5906.546 kb on + strand, within BT4489 at 5906.558 kb on - strand, within BT4489 at 5906.565 kb on + strand, within BT4489 at 5906.566 kb on - strand, within BT4489 at 5906.600 kb on - strand, within BT4489 at 5906.656 kb on + strand, within BT4489 at 5906.699 kb on - strand, within BT4489 at 5906.898 kb on - strand, within BT4489 at 5906.966 kb on + strand, within BT4489 at 5906.966 kb on + strand, within BT4489 at 5906.967 kb on - strand, within BT4489 at 5906.967 kb on - strand, within BT4489 at 5907.030 kb on + strand, within BT4489 at 5907.156 kb on - strand at 5907.166 kb on + strand at 5907.276 kb on - strand at 5907.323 kb on + strand at 5907.381 kb on - strand at 5907.401 kb on - strand, within BT4490 at 5907.517 kb on - strand at 5907.547 kb on + strand, within BT4490 at 5907.548 kb on - strand, within BT4490 at 5907.579 kb on + strand, within BT4490 at 5907.584 kb on + strand, within BT4490 at 5907.641 kb on + strand, within BT4490 at 5907.732 kb on + strand, within BT4490 at 5907.881 kb on - strand at 5907.897 kb on + strand at 5907.900 kb on - strand at 5907.914 kb on + strand at 5907.915 kb on - strand at 5907.932 kb on + strand, within BT4491 at 5907.944 kb on + strand, within BT4491 at 5907.945 kb on - strand, within BT4491 at 5907.970 kb on - strand, within BT4491 at 5907.973 kb on + strand, within BT4491 at 5908.018 kb on - strand, within BT4491 at 5908.041 kb on - strand, within BT4491 at 5908.107 kb on + strand, within BT4491 at 5908.107 kb on + strand, within BT4491 at 5908.107 kb on + strand, within BT4491 at 5908.108 kb on - strand, within BT4491 at 5908.113 kb on + strand, within BT4491 at 5908.113 kb on + strand, within BT4491 at 5908.114 kb on - strand, within BT4491 at 5908.114 kb on - strand, within BT4491 at 5908.155 kb on + strand, within BT4491 at 5908.194 kb on - strand, within BT4491 at 5908.198 kb on - strand, within BT4491 at 5908.219 kb on - strand, within BT4491
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 5,904,667 + BT4488 0.28 +0.1 5,904,707 + BT4488 0.33 -1.7 5,904,708 - BT4488 0.33 +0.0 5,904,721 + BT4488 0.35 +1.3 5,904,721 + BT4488 0.35 +0.4 5,904,722 - BT4488 0.35 +0.0 5,904,822 - BT4488 0.47 +0.6 5,904,914 + BT4488 0.58 +0.0 5,904,978 + BT4488 0.65 -1.9 5,904,979 - BT4488 0.65 -1.6 5,905,033 + BT4488 0.72 +0.0 5,905,071 + BT4488 0.76 -2.3 5,905,214 - -1.6 5,905,264 + +1.0 5,905,265 - -1.0 5,905,265 - +0.4 5,905,265 - +0.8 5,905,310 + -1.0 5,905,310 + +0.0 5,905,310 + -0.7 5,905,315 + -0.4 5,905,380 + -2.1 5,905,431 + -1.6 5,905,431 + +1.0 5,905,433 + -2.8 5,905,477 - -3.2 5,905,483 - -1.0 5,905,591 + +0.0 5,905,591 + +0.4 5,905,592 - +1.6 5,905,603 - +0.7 5,905,633 - +0.0 5,905,634 + -2.2 5,905,636 + -1.0 5,905,643 + +0.0 5,905,644 - -0.9 5,905,644 - +0.0 5,905,647 + -1.6 5,905,656 + -0.7 5,905,669 + -1.0 5,905,670 - -0.1 5,905,687 + -0.4 5,905,727 - -2.2 5,905,739 - -2.0 5,905,765 - -1.6 5,905,765 - -2.2 5,905,807 + -1.6 5,905,808 - +2.3 5,905,808 - -2.8 5,905,822 + BT4489 0.10 -1.0 5,905,823 - BT4489 0.11 +1.6 5,905,828 + BT4489 0.11 -0.5 5,905,829 - BT4489 0.11 -0.4 5,905,829 - BT4489 0.11 +0.3 5,905,863 - BT4489 0.13 +0.0 5,905,874 + BT4489 0.14 -1.6 5,905,886 - BT4489 0.14 -0.4 5,905,889 + BT4489 0.15 -2.7 5,905,889 + BT4489 0.15 -1.9 5,905,889 + BT4489 0.15 -1.6 5,905,950 - BT4489 0.18 -0.8 5,905,992 + BT4489 0.21 +0.0 5,906,033 + BT4489 0.24 +0.3 5,906,033 + BT4489 0.24 +0.0 5,906,111 + BT4489 0.29 -1.4 5,906,111 + BT4489 0.29 -1.8 5,906,137 + BT4489 0.30 -0.8 5,906,169 - BT4489 0.32 +0.0 5,906,401 + BT4489 0.47 -0.3 5,906,456 - BT4489 0.50 +0.9 5,906,479 - BT4489 0.52 -2.0 5,906,536 + BT4489 0.55 -0.6 5,906,536 + BT4489 0.55 -1.0 5,906,537 - BT4489 0.55 +1.6 5,906,537 - BT4489 0.55 +0.0 5,906,546 + BT4489 0.56 -1.6 5,906,558 - BT4489 0.56 -1.6 5,906,565 + BT4489 0.57 +0.1 5,906,566 - BT4489 0.57 -2.6 5,906,600 - BT4489 0.59 -0.5 5,906,656 + BT4489 0.63 -1.0 5,906,699 - BT4489 0.65 +1.2 5,906,898 - BT4489 0.78 +0.0 5,906,966 + BT4489 0.82 -1.0 5,906,966 + BT4489 0.82 -2.8 5,906,967 - BT4489 0.82 +0.0 5,906,967 - BT4489 0.82 +0.4 5,907,030 + BT4489 0.86 -0.6 5,907,156 - -2.3 5,907,166 + +0.0 5,907,276 - -1.0 5,907,323 + -0.5 5,907,381 - +0.0 5,907,401 - BT4490 0.19 -1.0 5,907,517 - -1.6 5,907,547 + BT4490 0.45 +0.6 5,907,548 - BT4490 0.45 -1.6 5,907,579 + BT4490 0.50 -2.3 5,907,584 + BT4490 0.51 -1.1 5,907,641 + BT4490 0.61 -1.0 5,907,732 + BT4490 0.77 +0.3 5,907,881 - -1.0 5,907,897 + +1.6 5,907,900 - +1.3 5,907,914 + -2.3 5,907,915 - +0.0 5,907,932 + BT4491 0.14 -3.0 5,907,944 + BT4491 0.17 -0.5 5,907,945 - BT4491 0.17 +0.0 5,907,970 - BT4491 0.24 -0.5 5,907,973 + BT4491 0.24 +0.0 5,908,018 - BT4491 0.36 +0.0 5,908,041 - BT4491 0.42 -1.5 5,908,107 + BT4491 0.59 -0.6 5,908,107 + BT4491 0.59 -2.6 5,908,107 + BT4491 0.59 +0.5 5,908,108 - BT4491 0.59 +0.0 5,908,113 + BT4491 0.60 -1.6 5,908,113 + BT4491 0.60 -2.8 5,908,114 - BT4491 0.61 +0.0 5,908,114 - BT4491 0.61 -1.2 5,908,155 + BT4491 0.71 -1.2 5,908,194 - BT4491 0.81 -2.6 5,908,198 - BT4491 0.82 -2.0 5,908,219 - BT4491 0.88 -0.6
Or see this region's nucleotide sequence