Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT_2070 and BT2071 are separated by 64 nucleotides BT2071 and BT2072 are separated by 14 nucleotides
BT_2070: BT_2070 - methylcitrate synthase (RefSeq), at 2,591,718 to 2,593,022
_2070
BT2071: BT2071 - isocitrate dehydrogenase [NADP] (NCBI ptt file), at 2,593,087 to 2,594,277
BT2071
BT2072: BT2072 - aconitate hydratase (NCBI ptt file), at 2,594,292 to 2,596,535
BT2072
Position (kb)
2593
2594
2595 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2592.103 kb on + strand, within BT_2070 at 2592.119 kb on + strand, within BT_2070 at 2592.119 kb on + strand, within BT_2070 at 2592.124 kb on + strand, within BT_2070 at 2592.154 kb on + strand, within BT_2070 at 2592.155 kb on - strand, within BT_2070 at 2592.155 kb on - strand, within BT_2070 at 2592.155 kb on - strand, within BT_2070 at 2592.158 kb on + strand, within BT_2070 at 2592.159 kb on - strand, within BT_2070 at 2592.162 kb on + strand, within BT_2070 at 2592.192 kb on - strand, within BT_2070 at 2592.216 kb on - strand, within BT_2070 at 2592.289 kb on + strand, within BT_2070 at 2592.289 kb on + strand, within BT_2070 at 2592.289 kb on + strand, within BT_2070 at 2592.290 kb on - strand, within BT_2070 at 2592.293 kb on + strand, within BT_2070 at 2592.294 kb on - strand, within BT_2070 at 2592.294 kb on - strand, within BT_2070 at 2592.422 kb on + strand, within BT_2070 at 2592.423 kb on - strand, within BT_2070 at 2592.509 kb on + strand, within BT_2070 at 2592.510 kb on - strand, within BT_2070 at 2592.512 kb on - strand, within BT_2070 at 2592.545 kb on + strand, within BT_2070 at 2592.545 kb on + strand, within BT_2070 at 2592.585 kb on - strand, within BT_2070 at 2592.638 kb on + strand, within BT_2070 at 2592.701 kb on + strand, within BT_2070 at 2592.702 kb on - strand, within BT_2070 at 2592.890 kb on + strand, within BT_2070 at 2592.903 kb on + strand at 2592.903 kb on + strand at 2592.936 kb on + strand at 2592.937 kb on - strand at 2593.048 kb on + strand at 2593.048 kb on + strand at 2593.048 kb on + strand at 2593.065 kb on - strand at 2593.065 kb on - strand at 2593.075 kb on + strand at 2593.131 kb on + strand at 2593.143 kb on + strand at 2593.144 kb on - strand at 2593.247 kb on + strand, within BT2071 at 2593.368 kb on + strand, within BT2071 at 2593.368 kb on + strand, within BT2071 at 2593.369 kb on - strand, within BT2071 at 2593.428 kb on - strand, within BT2071 at 2593.463 kb on + strand, within BT2071 at 2593.486 kb on - strand, within BT2071 at 2593.784 kb on + strand, within BT2071 at 2593.785 kb on - strand, within BT2071 at 2593.832 kb on + strand, within BT2071 at 2593.832 kb on + strand, within BT2071 at 2593.832 kb on + strand, within BT2071 at 2593.833 kb on - strand, within BT2071 at 2593.833 kb on - strand, within BT2071 at 2593.834 kb on + strand, within BT2071 at 2593.835 kb on - strand, within BT2071 at 2593.835 kb on - strand, within BT2071 at 2593.835 kb on - strand, within BT2071 at 2593.835 kb on - strand, within BT2071 at 2593.837 kb on - strand, within BT2071 at 2593.837 kb on - strand, within BT2071 at 2593.837 kb on - strand, within BT2071 at 2593.846 kb on - strand, within BT2071 at 2594.211 kb on + strand at 2594.213 kb on + strand at 2594.213 kb on + strand at 2594.213 kb on + strand at 2594.214 kb on - strand at 2594.248 kb on - strand at 2594.248 kb on - strand at 2594.266 kb on + strand at 2594.347 kb on + strand at 2594.348 kb on - strand at 2594.351 kb on + strand at 2594.351 kb on + strand at 2594.351 kb on + strand at 2594.352 kb on - strand at 2594.499 kb on - strand at 2594.702 kb on - strand, within BT2072 at 2594.766 kb on + strand, within BT2072 at 2594.772 kb on - strand, within BT2072 at 2594.843 kb on + strand, within BT2072 at 2594.877 kb on - strand, within BT2072 at 2594.883 kb on + strand, within BT2072 at 2594.885 kb on + strand, within BT2072 at 2594.981 kb on + strand, within BT2072 at 2595.239 kb on + strand, within BT2072
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 2,592,103 + BT_2070 0.30 -1.1 2,592,119 + BT_2070 0.31 -2.0 2,592,119 + BT_2070 0.31 -2.0 2,592,124 + BT_2070 0.31 -0.0 2,592,154 + BT_2070 0.33 -1.0 2,592,155 - BT_2070 0.33 +1.6 2,592,155 - BT_2070 0.33 -2.1 2,592,155 - BT_2070 0.33 -1.4 2,592,158 + BT_2070 0.34 -1.0 2,592,159 - BT_2070 0.34 -1.2 2,592,162 + BT_2070 0.34 -2.7 2,592,192 - BT_2070 0.36 -0.6 2,592,216 - BT_2070 0.38 -1.6 2,592,289 + BT_2070 0.44 -2.6 2,592,289 + BT_2070 0.44 -2.0 2,592,289 + BT_2070 0.44 -2.0 2,592,290 - BT_2070 0.44 -1.6 2,592,293 + BT_2070 0.44 -1.4 2,592,294 - BT_2070 0.44 +0.4 2,592,294 - BT_2070 0.44 -1.3 2,592,422 + BT_2070 0.54 -0.0 2,592,423 - BT_2070 0.54 -1.2 2,592,509 + BT_2070 0.61 -0.7 2,592,510 - BT_2070 0.61 -3.3 2,592,512 - BT_2070 0.61 -0.0 2,592,545 + BT_2070 0.63 -0.6 2,592,545 + BT_2070 0.63 -1.6 2,592,585 - BT_2070 0.66 -0.5 2,592,638 + BT_2070 0.70 -1.7 2,592,701 + BT_2070 0.75 -1.0 2,592,702 - BT_2070 0.75 -0.6 2,592,890 + BT_2070 0.90 -2.3 2,592,903 + -0.4 2,592,903 + -0.7 2,592,936 + -0.9 2,592,937 - -1.2 2,593,048 + -1.5 2,593,048 + -0.6 2,593,048 + -0.0 2,593,065 - -2.3 2,593,065 - -2.0 2,593,075 + -1.7 2,593,131 + -0.7 2,593,143 + -2.1 2,593,144 - -1.3 2,593,247 + BT2071 0.13 -0.0 2,593,368 + BT2071 0.24 -0.0 2,593,368 + BT2071 0.24 -2.0 2,593,369 - BT2071 0.24 -2.8 2,593,428 - BT2071 0.29 -0.0 2,593,463 + BT2071 0.32 -1.0 2,593,486 - BT2071 0.34 -2.3 2,593,784 + BT2071 0.59 -2.2 2,593,785 - BT2071 0.59 +1.0 2,593,832 + BT2071 0.63 -1.0 2,593,832 + BT2071 0.63 -0.4 2,593,832 + BT2071 0.63 -0.9 2,593,833 - BT2071 0.63 -1.6 2,593,833 - BT2071 0.63 -0.0 2,593,834 + BT2071 0.63 -0.0 2,593,835 - BT2071 0.63 +0.6 2,593,835 - BT2071 0.63 -1.3 2,593,835 - BT2071 0.63 -1.6 2,593,835 - BT2071 0.63 -2.6 2,593,837 - BT2071 0.63 -0.6 2,593,837 - BT2071 0.63 -0.0 2,593,837 - BT2071 0.63 -2.3 2,593,846 - BT2071 0.64 -1.6 2,594,211 + -1.0 2,594,213 + -2.3 2,594,213 + -3.2 2,594,213 + -2.2 2,594,214 - -1.2 2,594,248 - -1.0 2,594,248 - -1.0 2,594,266 + -2.0 2,594,347 + -2.6 2,594,348 - -2.0 2,594,351 + -0.0 2,594,351 + -2.2 2,594,351 + -2.0 2,594,352 - -2.6 2,594,499 - -0.7 2,594,702 - BT2072 0.18 -1.6 2,594,766 + BT2072 0.21 -1.4 2,594,772 - BT2072 0.21 -1.6 2,594,843 + BT2072 0.25 +0.5 2,594,877 - BT2072 0.26 -0.4 2,594,883 + BT2072 0.26 +0.5 2,594,885 + BT2072 0.26 -0.0 2,594,981 + BT2072 0.31 -0.2 2,595,239 + BT2072 0.42 +1.0
Or see this region's nucleotide sequence