Experiment: BHIS with Dimethyl Sulfoxide 0.2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0773 and BT0774 are separated by 51 nucleotides BT0774 and BT0775 are separated by 128 nucleotides BT0775 and BT0776 are separated by 6 nucleotides BT0776 and BT0777 are separated by 14 nucleotides
BT0773: BT0773 - alpha-amylase 3 (NCBI ptt file), at 963,212 to 964,909
BT0773
BT0774: BT0774 - conserved protein with a conserved patatin-like phospholipase domain (NCBI ptt file), at 964,961 to 965,767
BT0774
BT0775: BT0775 - ferredoxin-type protein (NCBI ptt file), at 965,896 to 967,461
BT0775
BT0776: BT0776 - conserved hypothetical protein (NCBI ptt file), at 967,468 to 968,403
BT0776
BT0777: BT0777 - hypothetical protein (NCBI ptt file), at 968,418 to 968,933
BT0777
Position (kb) 965
966
967
968 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 964.917 kb on - strand at 965.020 kb on + strand at 965.022 kb on + strand at 965.077 kb on - strand, within BT0774 at 965.210 kb on + strand, within BT0774 at 965.387 kb on - strand, within BT0774 at 965.557 kb on - strand, within BT0774 at 965.632 kb on + strand, within BT0774 at 965.648 kb on - strand, within BT0774 at 965.699 kb on - strand at 965.719 kb on - strand at 965.724 kb on - strand at 965.735 kb on - strand at 965.801 kb on + strand at 965.985 kb on - strand at 965.986 kb on + strand at 965.987 kb on - strand at 965.987 kb on - strand at 966.083 kb on + strand, within BT0775 at 966.093 kb on + strand, within BT0775 at 966.172 kb on + strand, within BT0775 at 966.175 kb on - strand, within BT0775 at 966.504 kb on + strand at 966.504 kb on + strand, within BT0775 at 966.712 kb on - strand, within BT0775 at 966.712 kb on - strand, within BT0775 at 966.712 kb on - strand, within BT0775 at 966.868 kb on - strand, within BT0775 at 966.898 kb on + strand, within BT0775 at 966.964 kb on + strand, within BT0775 at 966.964 kb on + strand, within BT0775 at 967.001 kb on - strand, within BT0775 at 967.031 kb on + strand, within BT0775 at 967.048 kb on - strand, within BT0775 at 967.052 kb on - strand, within BT0775 at 967.052 kb on - strand, within BT0775 at 967.054 kb on + strand, within BT0775 at 967.055 kb on - strand, within BT0775 at 967.077 kb on - strand, within BT0775 at 967.225 kb on - strand, within BT0775 at 967.258 kb on - strand, within BT0775 at 967.265 kb on + strand, within BT0775 at 967.269 kb on + strand, within BT0775 at 967.269 kb on + strand, within BT0775 at 967.270 kb on - strand, within BT0775 at 967.301 kb on - strand, within BT0775 at 967.309 kb on + strand at 967.309 kb on + strand at 967.315 kb on + strand at 967.327 kb on + strand at 967.476 kb on + strand at 967.607 kb on - strand, within BT0776 at 967.695 kb on + strand, within BT0776 at 967.717 kb on - strand, within BT0776 at 967.804 kb on - strand, within BT0776 at 968.062 kb on + strand, within BT0776 at 968.347 kb on + strand at 968.348 kb on - strand at 968.349 kb on + strand at 968.371 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 0.2 vol% remove 964,917 - -0.2 965,020 + +0.2 965,022 + -2.9 965,077 - BT0774 0.14 -2.2 965,210 + BT0774 0.31 -1.4 965,387 - BT0774 0.53 -0.2 965,557 - BT0774 0.74 -0.8 965,632 + BT0774 0.83 +0.0 965,648 - BT0774 0.85 -0.8 965,699 - -1.4 965,719 - -1.2 965,724 - +3.3 965,735 - +0.2 965,801 + +0.2 965,985 - -0.3 965,986 + -0.2 965,987 - -0.0 965,987 - -0.6 966,083 + BT0775 0.12 -1.4 966,093 + BT0775 0.13 -2.4 966,172 + BT0775 0.18 -3.5 966,175 - BT0775 0.18 -0.8 966,504 + -0.4 966,504 + BT0775 0.39 -1.4 966,712 - BT0775 0.52 -1.1 966,712 - BT0775 0.52 -0.3 966,712 - BT0775 0.52 -0.8 966,868 - BT0775 0.62 +0.2 966,898 + BT0775 0.64 +0.2 966,964 + BT0775 0.68 -1.1 966,964 + BT0775 0.68 -0.8 967,001 - BT0775 0.71 -0.4 967,031 + BT0775 0.72 -1.8 967,048 - BT0775 0.74 -1.4 967,052 - BT0775 0.74 -2.6 967,052 - BT0775 0.74 -2.4 967,054 + BT0775 0.74 +0.2 967,055 - BT0775 0.74 +0.2 967,077 - BT0775 0.75 -0.8 967,225 - BT0775 0.85 -1.5 967,258 - BT0775 0.87 -1.8 967,265 + BT0775 0.87 +0.2 967,269 + BT0775 0.88 -0.8 967,269 + BT0775 0.88 +0.2 967,270 - BT0775 0.88 -3.3 967,301 - BT0775 0.90 -1.1 967,309 + +0.1 967,309 + +0.1 967,315 + -1.4 967,327 + -0.9 967,476 + -0.2 967,607 - BT0776 0.15 -0.9 967,695 + BT0776 0.24 -1.1 967,717 - BT0776 0.27 +0.1 967,804 - BT0776 0.36 +0.1 968,062 + BT0776 0.63 -1.4 968,347 + -3.3 968,348 - -2.9 968,349 + -1.4 968,371 - +0.1
Or see this region's nucleotide sequence