Experiment: Grazed flask 8 outgrowth 150uE (no effect)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0342 and psb27 are separated by 63 nucleotides psb27 and Synpcc7942_0344 are separated by 352 nucleotides Synpcc7942_0344 and birA are separated by 84 nucleotides birA and Synpcc7942_0346 are separated by 45 nucleotides
Synpcc7942_0342: Synpcc7942_0342 - hypothetical protein, at 336,363 to 336,590
_0342
Synpcc7942_0343: psb27 - photosystem II 11 kD protein, at 336,654 to 337,055
psb27
Synpcc7942_0344: Synpcc7942_0344 - probable peptidase, at 337,408 to 338,451
_0344
Synpcc7942_0345: birA - Biotin--acetyl-CoA-carboxylase ligase, at 338,536 to 339,306
birA
Synpcc7942_0346: Synpcc7942_0346 - Protein of unknown function DUF152, at 339,352 to 340,131
_0346
Position (kb)
337
338
339 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 336.425 kb on - strand, within Synpcc7942_0342 at 336.452 kb on + strand, within Synpcc7942_0342 at 336.468 kb on + strand, within Synpcc7942_0342 at 336.498 kb on + strand, within Synpcc7942_0342 at 336.504 kb on + strand, within Synpcc7942_0342 at 336.506 kb on - strand, within Synpcc7942_0342 at 336.512 kb on - strand, within Synpcc7942_0342 at 336.544 kb on - strand, within Synpcc7942_0342 at 336.544 kb on - strand, within Synpcc7942_0342 at 336.563 kb on - strand, within Synpcc7942_0342 at 336.590 kb on + strand at 336.640 kb on + strand at 336.769 kb on - strand, within psb27 at 336.774 kb on + strand, within psb27 at 336.774 kb on + strand, within psb27 at 336.814 kb on - strand, within psb27 at 336.838 kb on + strand, within psb27 at 336.933 kb on + strand, within psb27 at 337.050 kb on + strand at 337.050 kb on + strand at 337.050 kb on + strand at 337.058 kb on - strand at 337.058 kb on - strand at 337.058 kb on - strand at 337.058 kb on - strand at 337.058 kb on - strand at 337.101 kb on + strand at 337.107 kb on + strand at 337.200 kb on + strand at 337.200 kb on + strand at 337.200 kb on + strand at 337.201 kb on + strand at 337.201 kb on + strand at 337.201 kb on + strand at 337.201 kb on + strand at 337.201 kb on + strand at 337.201 kb on + strand at 337.201 kb on + strand at 337.201 kb on + strand at 337.209 kb on - strand at 337.209 kb on - strand at 337.209 kb on - strand at 337.209 kb on - strand at 337.209 kb on - strand at 337.222 kb on - strand at 337.224 kb on + strand at 337.236 kb on + strand at 337.245 kb on - strand at 337.249 kb on + strand at 337.257 kb on + strand at 337.257 kb on + strand at 337.327 kb on + strand at 337.364 kb on + strand at 337.405 kb on + strand at 337.405 kb on + strand at 337.412 kb on + strand at 337.412 kb on + strand at 337.413 kb on - strand at 337.420 kb on - strand at 337.431 kb on + strand at 337.442 kb on + strand at 337.486 kb on + strand at 337.489 kb on + strand at 337.540 kb on + strand, within Synpcc7942_0344 at 337.563 kb on - strand, within Synpcc7942_0344 at 337.593 kb on - strand, within Synpcc7942_0344 at 337.707 kb on + strand, within Synpcc7942_0344 at 337.719 kb on - strand, within Synpcc7942_0344 at 337.814 kb on + strand, within Synpcc7942_0344 at 337.821 kb on - strand, within Synpcc7942_0344 at 337.821 kb on - strand, within Synpcc7942_0344 at 337.846 kb on + strand, within Synpcc7942_0344 at 337.854 kb on - strand, within Synpcc7942_0344 at 337.854 kb on - strand, within Synpcc7942_0344 at 337.870 kb on + strand, within Synpcc7942_0344 at 337.870 kb on + strand, within Synpcc7942_0344 at 337.880 kb on + strand at 337.951 kb on - strand, within Synpcc7942_0344 at 337.958 kb on + strand, within Synpcc7942_0344 at 337.966 kb on - strand, within Synpcc7942_0344 at 337.980 kb on + strand, within Synpcc7942_0344 at 338.009 kb on + strand, within Synpcc7942_0344 at 338.009 kb on + strand, within Synpcc7942_0344 at 338.017 kb on - strand, within Synpcc7942_0344 at 338.042 kb on - strand, within Synpcc7942_0344 at 338.046 kb on - strand, within Synpcc7942_0344 at 338.121 kb on - strand, within Synpcc7942_0344 at 338.220 kb on + strand, within Synpcc7942_0344 at 338.281 kb on + strand, within Synpcc7942_0344 at 338.320 kb on - strand, within Synpcc7942_0344 at 338.320 kb on - strand, within Synpcc7942_0344 at 338.331 kb on - strand, within Synpcc7942_0344 at 338.379 kb on - strand at 338.402 kb on + strand at 338.405 kb on - strand at 338.421 kb on - strand at 338.435 kb on - strand at 338.435 kb on - strand at 338.437 kb on - strand at 338.446 kb on + strand at 338.454 kb on - strand at 338.454 kb on - strand at 338.463 kb on - strand at 338.464 kb on - strand at 338.480 kb on + strand at 338.485 kb on - strand at 338.488 kb on - strand at 338.492 kb on + strand at 338.492 kb on + strand at 338.515 kb on - strand at 338.534 kb on + strand at 338.566 kb on - strand at 339.336 kb on + strand at 339.344 kb on + strand at 339.371 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Grazed flask 8 outgrowth 150uE (no effect) remove 336,425 - Synpcc7942_0342 0.27 +0.8 336,452 + Synpcc7942_0342 0.39 +0.4 336,468 + Synpcc7942_0342 0.46 -3.5 336,498 + Synpcc7942_0342 0.59 -0.8 336,504 + Synpcc7942_0342 0.62 +0.3 336,506 - Synpcc7942_0342 0.63 +1.3 336,512 - Synpcc7942_0342 0.65 +0.1 336,544 - Synpcc7942_0342 0.79 +0.8 336,544 - Synpcc7942_0342 0.79 -0.9 336,563 - Synpcc7942_0342 0.88 +0.3 336,590 + -0.4 336,640 + +0.3 336,769 - psb27 Synpcc7942_0343 0.29 -0.6 336,774 + psb27 Synpcc7942_0343 0.30 -1.5 336,774 + psb27 Synpcc7942_0343 0.30 -0.9 336,814 - psb27 Synpcc7942_0343 0.40 +0.1 336,838 + psb27 Synpcc7942_0343 0.46 -1.3 336,933 + psb27 Synpcc7942_0343 0.69 -0.5 337,050 + +0.5 337,050 + +0.1 337,050 + -0.6 337,058 - +0.2 337,058 - +0.3 337,058 - -0.6 337,058 - +0.4 337,058 - +1.2 337,101 + -1.0 337,107 + +0.3 337,200 + +0.7 337,200 + +0.2 337,200 + -0.2 337,201 + -0.5 337,201 + -0.2 337,201 + +0.1 337,201 + +0.6 337,201 + +0.4 337,201 + -1.5 337,201 + +0.0 337,201 + -0.6 337,209 - -0.3 337,209 - +0.4 337,209 - -0.5 337,209 - +0.8 337,209 - -0.2 337,222 - -0.8 337,224 + +0.2 337,236 + +0.6 337,245 - +0.1 337,249 + +0.5 337,257 + +0.1 337,257 + +0.0 337,327 + +0.5 337,364 + -1.1 337,405 + +0.3 337,405 + -0.4 337,412 + +2.1 337,412 + +0.2 337,413 - -0.1 337,420 - -0.3 337,431 + +0.4 337,442 + -1.0 337,486 + -0.1 337,489 + -1.0 337,540 + Synpcc7942_0344 0.13 +0.6 337,563 - Synpcc7942_0344 0.15 -0.3 337,593 - Synpcc7942_0344 0.18 +0.1 337,707 + Synpcc7942_0344 0.29 +0.2 337,719 - Synpcc7942_0344 0.30 -1.9 337,814 + Synpcc7942_0344 0.39 -1.6 337,821 - Synpcc7942_0344 0.40 -1.3 337,821 - Synpcc7942_0344 0.40 -1.1 337,846 + Synpcc7942_0344 0.42 -0.1 337,854 - Synpcc7942_0344 0.43 -0.2 337,854 - Synpcc7942_0344 0.43 +1.2 337,870 + Synpcc7942_0344 0.44 -0.6 337,870 + Synpcc7942_0344 0.44 -0.5 337,880 + -0.9 337,951 - Synpcc7942_0344 0.52 -1.0 337,958 + Synpcc7942_0344 0.53 -1.9 337,966 - Synpcc7942_0344 0.53 +0.7 337,980 + Synpcc7942_0344 0.55 +0.6 338,009 + Synpcc7942_0344 0.58 -1.8 338,009 + Synpcc7942_0344 0.58 -0.5 338,017 - Synpcc7942_0344 0.58 -0.0 338,042 - Synpcc7942_0344 0.61 -2.2 338,046 - Synpcc7942_0344 0.61 -0.6 338,121 - Synpcc7942_0344 0.68 -0.7 338,220 + Synpcc7942_0344 0.78 -1.7 338,281 + Synpcc7942_0344 0.84 -0.7 338,320 - Synpcc7942_0344 0.87 -0.6 338,320 - Synpcc7942_0344 0.87 -0.2 338,331 - Synpcc7942_0344 0.88 -0.5 338,379 - -0.3 338,402 + -0.9 338,405 - -1.6 338,421 - -0.7 338,435 - -0.9 338,435 - -0.6 338,437 - -0.7 338,446 + +0.6 338,454 - +0.6 338,454 - -0.2 338,463 - +0.1 338,464 - -0.2 338,480 + -1.9 338,485 - -1.9 338,488 - +0.9 338,492 + +0.3 338,492 + -0.3 338,515 - +0.0 338,534 + -0.2 338,566 - -1.0 339,336 + +0.7 339,344 + +0.2 339,371 - -0.1
Or see this region's nucleotide sequence