Strain Fitness in Paraburkholderia graminis OAS925 around ABIE53_005229

Experiment: Shikimic Acid 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABIE53_005227 and ABIE53_005228 are separated by 29 nucleotidesABIE53_005228 and ABIE53_005229 overlap by 4 nucleotidesABIE53_005229 and ABIE53_005230 are separated by 192 nucleotidesABIE53_005230 and ABIE53_005231 overlap by 4 nucleotides ABIE53_005227: ABIE53_005227 - 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase, at 1,484,588 to 1,486,045 _005227 ABIE53_005228: ABIE53_005228 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase/2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 1,486,075 to 1,486,833 _005228 ABIE53_005229: ABIE53_005229 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase/2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 1,486,830 to 1,487,615 _005229 ABIE53_005230: ABIE53_005230 - homoprotocatechuate degradation regulator HpaR, at 1,487,808 to 1,488,215 _005230 ABIE53_005231: ABIE53_005231 - hypothetical protein, at 1,488,212 to 1,489,237 _005231 Position (kb) 1486 1487 1488Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1486.057 kb on + strandat 1486.376 kb on - strand, within ABIE53_005228at 1486.421 kb on + strand, within ABIE53_005228at 1486.422 kb on - strand, within ABIE53_005228at 1486.422 kb on - strand, within ABIE53_005228at 1486.501 kb on + strand, within ABIE53_005228at 1486.502 kb on - strand, within ABIE53_005228at 1486.502 kb on - strand, within ABIE53_005228at 1486.502 kb on - strand, within ABIE53_005228at 1486.547 kb on + strand, within ABIE53_005228at 1486.548 kb on - strand, within ABIE53_005228at 1486.749 kb on + strand, within ABIE53_005228at 1486.750 kb on - strand, within ABIE53_005228at 1487.020 kb on + strand, within ABIE53_005229at 1487.020 kb on + strand, within ABIE53_005229at 1487.020 kb on + strand, within ABIE53_005229at 1487.020 kb on + strand, within ABIE53_005229at 1487.179 kb on + strand, within ABIE53_005229at 1487.182 kb on - strand, within ABIE53_005229at 1487.390 kb on - strand, within ABIE53_005229at 1487.390 kb on - strand, within ABIE53_005229at 1487.390 kb on - strand, within ABIE53_005229at 1487.419 kb on + strand, within ABIE53_005229at 1487.420 kb on - strand, within ABIE53_005229at 1487.485 kb on + strand, within ABIE53_005229at 1487.486 kb on - strand, within ABIE53_005229at 1487.486 kb on - strand, within ABIE53_005229at 1487.682 kb on + strandat 1487.762 kb on + strandat 1487.762 kb on + strandat 1487.762 kb on + strandat 1487.763 kb on - strandat 1487.940 kb on - strand, within ABIE53_005230at 1487.994 kb on - strand, within ABIE53_005230at 1488.165 kb on + strand, within ABIE53_005230at 1488.223 kb on - strandat 1488.300 kb on - strandat 1488.302 kb on + strandat 1488.302 kb on + strandat 1488.303 kb on - strandat 1488.303 kb on - strandat 1488.313 kb on + strandat 1488.402 kb on - strand, within ABIE53_005231at 1488.413 kb on + strand, within ABIE53_005231at 1488.524 kb on + strand, within ABIE53_005231at 1488.525 kb on - strand, within ABIE53_005231at 1488.525 kb on - strand, within ABIE53_005231

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Per-strain Table

Position Strand Gene LocusTag Fraction Shikimic Acid 5 mM (C)
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1,486,057 + -0.4
1,486,376 - ABIE53_005228 0.40 -0.2
1,486,421 + ABIE53_005228 0.46 -0.8
1,486,422 - ABIE53_005228 0.46 +1.8
1,486,422 - ABIE53_005228 0.46 +0.8
1,486,501 + ABIE53_005228 0.56 -1.0
1,486,502 - ABIE53_005228 0.56 -1.8
1,486,502 - ABIE53_005228 0.56 +0.8
1,486,502 - ABIE53_005228 0.56 -1.6
1,486,547 + ABIE53_005228 0.62 +0.4
1,486,548 - ABIE53_005228 0.62 +0.8
1,486,749 + ABIE53_005228 0.89 +0.8
1,486,750 - ABIE53_005228 0.89 +0.8
1,487,020 + ABIE53_005229 0.24 -2.8
1,487,020 + ABIE53_005229 0.24 -2.1
1,487,020 + ABIE53_005229 0.24 +0.2
1,487,020 + ABIE53_005229 0.24 -0.2
1,487,179 + ABIE53_005229 0.44 +1.4
1,487,182 - ABIE53_005229 0.45 -0.5
1,487,390 - ABIE53_005229 0.71 +1.7
1,487,390 - ABIE53_005229 0.71 +0.6
1,487,390 - ABIE53_005229 0.71 -1.2
1,487,419 + ABIE53_005229 0.75 -2.3
1,487,420 - ABIE53_005229 0.75 -2.0
1,487,485 + ABIE53_005229 0.83 -0.8
1,487,486 - ABIE53_005229 0.83 +0.8
1,487,486 - ABIE53_005229 0.83 +2.4
1,487,682 + +0.2
1,487,762 + -0.4
1,487,762 + -1.5
1,487,762 + +2.4
1,487,763 - +1.8
1,487,940 - ABIE53_005230 0.32 -1.8
1,487,994 - ABIE53_005230 0.46 -0.8
1,488,165 + ABIE53_005230 0.88 -3.2
1,488,223 - +0.8
1,488,300 - +0.8
1,488,302 + +0.2
1,488,302 + +0.0
1,488,303 - +0.8
1,488,303 - -1.6
1,488,313 + +0.6
1,488,402 - ABIE53_005231 0.19 -2.8
1,488,413 + ABIE53_005231 0.20 +0.1
1,488,524 + ABIE53_005231 0.30 +1.8
1,488,525 - ABIE53_005231 0.31 +1.4
1,488,525 - ABIE53_005231 0.31 -1.5

Or see this region's nucleotide sequence