Experiment: BHIS with Dimetridazole 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3768 and BT3769 are separated by 124 nucleotides BT3769 and tRNA-Met are separated by 321 nucleotides tRNA-Met and BT3770 are separated by 238 nucleotides
BT3768: BT3768 - transcriptional regulator (NCBI ptt file), at 4,889,117 to 4,890,016
BT3768
BT3769: BT3769 - hypothetical protein (NCBI ptt file), at 4,890,141 to 4,890,572
BT3769
BT_t59: tRNA-Met - tRNA-Met (RefSeq), at 4,890,894 to 4,890,966
tRNA-Met
BT3770: BT3770 - transcriptional regulator (NCBI ptt file), at 4,891,205 to 4,891,795
BT3770
Position (kb)
4890
4891 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4889.316 kb on - strand, within BT3768 at 4889.316 kb on - strand, within BT3768 at 4889.317 kb on + strand, within BT3768 at 4889.320 kb on + strand, within BT3768 at 4889.321 kb on - strand, within BT3768 at 4889.354 kb on - strand, within BT3768 at 4889.418 kb on - strand, within BT3768 at 4889.418 kb on - strand, within BT3768 at 4889.454 kb on - strand, within BT3768 at 4889.539 kb on + strand, within BT3768 at 4889.539 kb on + strand, within BT3768 at 4889.598 kb on + strand, within BT3768 at 4889.598 kb on + strand, within BT3768 at 4889.612 kb on + strand, within BT3768 at 4889.628 kb on + strand, within BT3768 at 4889.628 kb on + strand, within BT3768 at 4889.629 kb on - strand, within BT3768 at 4889.668 kb on + strand, within BT3768 at 4889.669 kb on - strand, within BT3768 at 4889.719 kb on - strand, within BT3768 at 4889.719 kb on - strand, within BT3768 at 4889.723 kb on - strand, within BT3768 at 4889.786 kb on + strand, within BT3768 at 4889.817 kb on - strand, within BT3768 at 4889.819 kb on - strand, within BT3768 at 4889.977 kb on + strand at 4889.978 kb on - strand at 4890.027 kb on - strand at 4890.082 kb on + strand at 4890.083 kb on - strand at 4890.085 kb on - strand at 4890.167 kb on - strand at 4890.270 kb on - strand, within BT3769 at 4890.282 kb on + strand, within BT3769 at 4890.314 kb on - strand, within BT3769 at 4890.329 kb on + strand, within BT3769 at 4890.329 kb on + strand, within BT3769 at 4890.330 kb on - strand, within BT3769 at 4890.378 kb on - strand, within BT3769 at 4890.399 kb on - strand, within BT3769 at 4890.451 kb on - strand, within BT3769 at 4890.473 kb on + strand, within BT3769 at 4890.494 kb on + strand, within BT3769 at 4890.499 kb on - strand, within BT3769 at 4890.570 kb on + strand at 4890.582 kb on + strand at 4890.582 kb on + strand at 4890.617 kb on + strand at 4890.626 kb on - strand at 4890.626 kb on - strand at 4890.626 kb on - strand at 4890.629 kb on - strand at 4890.629 kb on - strand at 4890.736 kb on - strand at 4890.818 kb on - strand at 4891.048 kb on + strand at 4891.078 kb on - strand at 4891.106 kb on - strand at 4891.148 kb on + strand at 4891.148 kb on + strand at 4891.197 kb on + strand at 4891.197 kb on + strand at 4891.342 kb on + strand, within BT3770 at 4891.534 kb on + strand, within BT3770
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.00625 mM remove 4,889,316 - BT3768 0.22 -1.3 4,889,316 - BT3768 0.22 -0.5 4,889,317 + BT3768 0.22 +0.3 4,889,320 + BT3768 0.23 +0.1 4,889,321 - BT3768 0.23 +0.1 4,889,354 - BT3768 0.26 +0.3 4,889,418 - BT3768 0.33 -1.0 4,889,418 - BT3768 0.33 -2.6 4,889,454 - BT3768 0.37 -1.6 4,889,539 + BT3768 0.47 -0.2 4,889,539 + BT3768 0.47 +0.3 4,889,598 + BT3768 0.53 -0.7 4,889,598 + BT3768 0.53 -0.7 4,889,612 + BT3768 0.55 -2.7 4,889,628 + BT3768 0.57 -0.9 4,889,628 + BT3768 0.57 +0.3 4,889,629 - BT3768 0.57 -2.5 4,889,668 + BT3768 0.61 -1.5 4,889,669 - BT3768 0.61 -1.3 4,889,719 - BT3768 0.67 +0.3 4,889,719 - BT3768 0.67 -2.3 4,889,723 - BT3768 0.67 -3.2 4,889,786 + BT3768 0.74 -2.4 4,889,817 - BT3768 0.78 -0.7 4,889,819 - BT3768 0.78 +0.3 4,889,977 + -0.7 4,889,978 - +0.3 4,890,027 - +1.3 4,890,082 + -1.6 4,890,083 - +0.3 4,890,085 - -0.7 4,890,167 - -0.7 4,890,270 - BT3769 0.30 -1.3 4,890,282 + BT3769 0.33 +0.3 4,890,314 - BT3769 0.40 -0.7 4,890,329 + BT3769 0.44 +0.3 4,890,329 + BT3769 0.44 -0.2 4,890,330 - BT3769 0.44 -0.2 4,890,378 - BT3769 0.55 -0.7 4,890,399 - BT3769 0.60 +0.9 4,890,451 - BT3769 0.72 +0.6 4,890,473 + BT3769 0.77 +0.3 4,890,494 + BT3769 0.82 -0.7 4,890,499 - BT3769 0.83 -0.1 4,890,570 + -1.5 4,890,582 + +0.3 4,890,582 + +0.3 4,890,617 + +0.3 4,890,626 - -3.2 4,890,626 - -2.5 4,890,626 - +0.3 4,890,629 - -1.7 4,890,629 - +0.3 4,890,736 - -0.7 4,890,818 - -0.6 4,891,048 + -1.3 4,891,078 - -1.6 4,891,106 - +0.3 4,891,148 + -0.7 4,891,148 + -1.8 4,891,197 + -2.0 4,891,197 + +0.1 4,891,342 + BT3770 0.23 +0.3 4,891,534 + BT3770 0.56 +2.0
Or see this region's nucleotide sequence