Experiment: BHIS with Dimetridazole 0.00625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1447 and BT1448 are separated by 91 nucleotides BT1448 and BT1449 are separated by 65 nucleotides BT1449 and BT1450 are separated by 55 nucleotides
BT1447: BT1447 - two-component system sensor histidine kinase (NCBI ptt file), at 1,781,157 to 1,783,514
BT1447
BT1448: BT1448 - biotin carboxyl carrier protein (NCBI ptt file), at 1,783,606 to 1,784,130
BT1448
BT1449: BT1449 - biotin carboxylase (NCBI ptt file), at 1,784,196 to 1,785,707
BT1449
BT1450: BT1450 - propionyl-CoA carboxylase beta chain (NCBI ptt file), at 1,785,763 to 1,787,307
BT1450
Position (kb)
1784
1785
1786 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1783.211 kb on - strand, within BT1447 at 1783.359 kb on + strand at 1783.372 kb on + strand at 1783.372 kb on + strand at 1783.412 kb on + strand at 1783.463 kb on + strand at 1783.514 kb on + strand at 1783.529 kb on + strand at 1783.533 kb on + strand at 1783.533 kb on + strand at 1783.534 kb on - strand at 1783.541 kb on + strand at 1783.772 kb on + strand, within BT1448 at 1783.773 kb on - strand, within BT1448 at 1783.849 kb on - strand, within BT1448 at 1783.852 kb on + strand, within BT1448 at 1783.873 kb on + strand, within BT1448 at 1783.874 kb on - strand, within BT1448 at 1783.874 kb on - strand, within BT1448 at 1784.015 kb on - strand, within BT1448 at 1784.070 kb on + strand, within BT1448 at 1784.070 kb on + strand, within BT1448 at 1784.073 kb on - strand, within BT1448 at 1784.089 kb on - strand at 1784.109 kb on - strand at 1784.124 kb on + strand at 1784.125 kb on - strand at 1784.160 kb on + strand at 1784.193 kb on + strand at 1784.204 kb on + strand at 1784.405 kb on - strand, within BT1449 at 1784.481 kb on - strand, within BT1449 at 1784.743 kb on + strand, within BT1449 at 1784.802 kb on - strand, within BT1449 at 1784.827 kb on - strand, within BT1449 at 1784.827 kb on - strand, within BT1449 at 1784.905 kb on + strand, within BT1449 at 1785.058 kb on + strand, within BT1449 at 1785.058 kb on + strand, within BT1449 at 1785.106 kb on + strand, within BT1449 at 1785.107 kb on - strand, within BT1449 at 1785.116 kb on + strand, within BT1449 at 1785.117 kb on - strand, within BT1449 at 1785.248 kb on + strand at 1785.248 kb on + strand, within BT1449 at 1785.256 kb on - strand, within BT1449 at 1785.258 kb on + strand, within BT1449 at 1785.537 kb on - strand, within BT1449 at 1785.666 kb on - strand at 1785.669 kb on + strand at 1785.762 kb on - strand at 1785.881 kb on + strand at 1785.991 kb on + strand, within BT1450 at 1785.997 kb on - strand, within BT1450 at 1786.092 kb on - strand, within BT1450 at 1786.104 kb on - strand, within BT1450 at 1786.110 kb on + strand, within BT1450 at 1786.176 kb on + strand, within BT1450 at 1786.249 kb on + strand, within BT1450 at 1786.252 kb on - strand, within BT1450 at 1786.374 kb on + strand, within BT1450 at 1786.447 kb on + strand, within BT1450 at 1786.461 kb on + strand, within BT1450 at 1786.461 kb on + strand, within BT1450 at 1786.461 kb on + strand, within BT1450 at 1786.561 kb on - strand, within BT1450 at 1786.561 kb on - strand, within BT1450 at 1786.585 kb on - strand, within BT1450 at 1786.598 kb on + strand, within BT1450 at 1786.599 kb on - strand, within BT1450 at 1786.599 kb on - strand, within BT1450 at 1786.605 kb on + strand, within BT1450
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.00625 mM remove 1,783,211 - BT1447 0.87 -1.3 1,783,359 + -0.3 1,783,372 + +0.7 1,783,372 + -0.5 1,783,412 + +2.7 1,783,463 + -0.9 1,783,514 + +0.1 1,783,529 + -1.3 1,783,533 + -1.3 1,783,533 + -0.3 1,783,534 - -0.3 1,783,541 + +0.7 1,783,772 + BT1448 0.32 -1.4 1,783,773 - BT1448 0.32 -0.8 1,783,849 - BT1448 0.46 -1.3 1,783,852 + BT1448 0.47 +0.7 1,783,873 + BT1448 0.51 -3.8 1,783,874 - BT1448 0.51 -2.8 1,783,874 - BT1448 0.51 -0.5 1,784,015 - BT1448 0.78 -0.3 1,784,070 + BT1448 0.88 -0.3 1,784,070 + BT1448 0.88 +0.7 1,784,073 - BT1448 0.89 -0.5 1,784,089 - -1.6 1,784,109 - +2.7 1,784,124 + -2.3 1,784,125 - -0.9 1,784,160 + -2.1 1,784,193 + +1.7 1,784,204 + -3.4 1,784,405 - BT1449 0.14 -3.1 1,784,481 - BT1449 0.19 -1.8 1,784,743 + BT1449 0.36 -2.1 1,784,802 - BT1449 0.40 -1.3 1,784,827 - BT1449 0.42 -1.3 1,784,827 - BT1449 0.42 +1.3 1,784,905 + BT1449 0.47 -1.1 1,785,058 + BT1449 0.57 -0.1 1,785,058 + BT1449 0.57 +0.2 1,785,106 + BT1449 0.60 -2.4 1,785,107 - BT1449 0.60 -0.3 1,785,116 + BT1449 0.61 -0.3 1,785,117 - BT1449 0.61 -0.3 1,785,248 + +0.7 1,785,248 + BT1449 0.70 -1.9 1,785,256 - BT1449 0.70 -0.3 1,785,258 + BT1449 0.70 -2.6 1,785,537 - BT1449 0.89 +0.1 1,785,666 - -1.3 1,785,669 + -0.3 1,785,762 - +0.7 1,785,881 + -0.3 1,785,991 + BT1450 0.15 -0.9 1,785,997 - BT1450 0.15 -1.1 1,786,092 - BT1450 0.21 -1.3 1,786,104 - BT1450 0.22 -2.5 1,786,110 + BT1450 0.22 -1.6 1,786,176 + BT1450 0.27 -1.5 1,786,249 + BT1450 0.31 -0.5 1,786,252 - BT1450 0.32 -0.6 1,786,374 + BT1450 0.40 -2.2 1,786,447 + BT1450 0.44 -1.3 1,786,461 + BT1450 0.45 -2.5 1,786,461 + BT1450 0.45 +1.3 1,786,461 + BT1450 0.45 -0.9 1,786,561 - BT1450 0.52 -2.1 1,786,561 - BT1450 0.52 -0.9 1,786,585 - BT1450 0.53 -0.9 1,786,598 + BT1450 0.54 -2.3 1,786,599 - BT1450 0.54 -0.9 1,786,599 - BT1450 0.54 -0.3 1,786,605 + BT1450 0.54 +0.7
Or see this region's nucleotide sequence