Experiment: Grazed flask 4 outgrowth 150uE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbC and rfbD overlap by 4 nucleotides rfbD and rfbB overlap by 4 nucleotides rfbB and rfbA overlap by 4 nucleotides rfbA and Synpcc7942_2102 are separated by 11 nucleotides
Synpcc7942_2098: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase, at 2,180,457 to 2,181,008
rfbC
Synpcc7942_2099: rfbD - dTDP-4-dehydrorhamnose reductase, at 2,181,005 to 2,181,874
rfbD
Synpcc7942_2100: rfbB - dTDP-glucose 4,6-dehydratase, at 2,181,871 to 2,182,944
rfbB
Synpcc7942_2101: rfbA - glucose-1-phosphate thymidylyltransferase, at 2,182,941 to 2,183,825
rfbA
Synpcc7942_2102: Synpcc7942_2102 - NAD-reducing hydrogenase gamma, at 2,183,837 to 2,184,076
_2102
Position (kb)
2181
2182
2183 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2180.891 kb on + strand, within rfbC at 2180.899 kb on - strand, within rfbC at 2180.946 kb on + strand, within rfbC at 2180.954 kb on - strand at 2180.981 kb on + strand at 2181.019 kb on + strand at 2181.027 kb on + strand at 2181.037 kb on + strand at 2181.045 kb on - strand at 2181.174 kb on - strand, within rfbD at 2181.179 kb on + strand, within rfbD at 2181.207 kb on - strand, within rfbD at 2181.328 kb on + strand, within rfbD at 2181.342 kb on - strand, within rfbD at 2181.382 kb on + strand, within rfbD at 2181.449 kb on + strand, within rfbD at 2181.553 kb on + strand, within rfbD at 2181.561 kb on - strand, within rfbD at 2181.614 kb on + strand, within rfbD at 2181.622 kb on - strand, within rfbD at 2181.710 kb on + strand, within rfbD at 2181.718 kb on - strand, within rfbD at 2181.727 kb on + strand, within rfbD at 2181.763 kb on + strand, within rfbD at 2181.764 kb on + strand, within rfbD at 2181.773 kb on + strand, within rfbD at 2181.786 kb on - strand, within rfbD at 2181.815 kb on + strand at 2181.823 kb on - strand at 2181.890 kb on - strand at 2181.913 kb on + strand at 2181.921 kb on - strand at 2181.935 kb on - strand at 2182.006 kb on - strand, within rfbB at 2182.007 kb on + strand, within rfbB at 2182.015 kb on - strand, within rfbB at 2182.015 kb on - strand, within rfbB at 2182.015 kb on - strand, within rfbB at 2182.038 kb on - strand, within rfbB at 2182.053 kb on + strand, within rfbB at 2182.093 kb on - strand, within rfbB at 2182.093 kb on - strand, within rfbB at 2182.107 kb on - strand, within rfbB at 2182.152 kb on - strand, within rfbB at 2182.202 kb on - strand, within rfbB at 2182.232 kb on + strand, within rfbB at 2182.258 kb on + strand, within rfbB at 2182.271 kb on - strand, within rfbB at 2182.278 kb on + strand, within rfbB at 2182.279 kb on + strand, within rfbB at 2182.286 kb on - strand, within rfbB at 2182.287 kb on - strand, within rfbB at 2182.362 kb on - strand, within rfbB at 2182.383 kb on + strand, within rfbB at 2182.395 kb on + strand, within rfbB at 2182.403 kb on - strand, within rfbB at 2182.405 kb on - strand, within rfbB at 2182.405 kb on - strand, within rfbB at 2182.457 kb on - strand, within rfbB at 2182.475 kb on - strand, within rfbB at 2182.489 kb on - strand, within rfbB at 2182.566 kb on - strand, within rfbB at 2182.571 kb on + strand, within rfbB at 2182.584 kb on - strand, within rfbB at 2182.584 kb on - strand, within rfbB at 2182.617 kb on - strand, within rfbB at 2182.641 kb on - strand, within rfbB at 2182.762 kb on + strand, within rfbB at 2182.921 kb on - strand at 2182.968 kb on - strand at 2182.991 kb on + strand at 2182.991 kb on + strand at 2182.991 kb on + strand at 2182.999 kb on - strand at 2183.003 kb on + strand at 2183.005 kb on + strand at 2183.005 kb on + strand at 2183.013 kb on - strand at 2183.013 kb on - strand at 2183.046 kb on - strand, within rfbA at 2183.063 kb on + strand, within rfbA at 2183.115 kb on + strand, within rfbA at 2183.115 kb on + strand, within rfbA at 2183.115 kb on + strand, within rfbA at 2183.115 kb on - strand, within rfbA at 2183.115 kb on - strand, within rfbA at 2183.123 kb on - strand, within rfbA at 2183.123 kb on - strand, within rfbA at 2183.141 kb on + strand, within rfbA at 2183.177 kb on + strand, within rfbA at 2183.177 kb on + strand, within rfbA at 2183.207 kb on - strand, within rfbA at 2183.214 kb on - strand, within rfbA at 2183.238 kb on + strand, within rfbA at 2183.338 kb on + strand, within rfbA at 2183.383 kb on - strand, within rfbA at 2183.432 kb on + strand, within rfbA at 2183.432 kb on + strand, within rfbA at 2183.457 kb on + strand, within rfbA at 2183.466 kb on + strand, within rfbA at 2183.501 kb on + strand, within rfbA at 2183.513 kb on - strand, within rfbA at 2183.540 kb on - strand, within rfbA at 2183.549 kb on + strand, within rfbA at 2183.557 kb on - strand, within rfbA at 2183.574 kb on + strand, within rfbA at 2183.592 kb on - strand, within rfbA at 2183.609 kb on + strand, within rfbA at 2183.628 kb on + strand, within rfbA at 2183.628 kb on + strand, within rfbA at 2183.636 kb on - strand, within rfbA at 2183.636 kb on - strand, within rfbA at 2183.721 kb on + strand, within rfbA at 2183.740 kb on - strand at 2183.746 kb on + strand at 2183.754 kb on - strand at 2183.763 kb on + strand at 2183.771 kb on - strand at 2183.804 kb on - strand at 2183.813 kb on - strand at 2183.827 kb on - strand at 2183.841 kb on + strand at 2183.849 kb on + strand at 2183.849 kb on - strand at 2183.862 kb on + strand, within Synpcc7942_2102 at 2183.897 kb on + strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.905 kb on - strand, within Synpcc7942_2102 at 2183.924 kb on + strand, within Synpcc7942_2102 at 2183.932 kb on - strand, within Synpcc7942_2102
Per-strain Table
Position Strand Gene LocusTag Fraction Grazed flask 4 outgrowth 150uE remove 2,180,891 + rfbC Synpcc7942_2098 0.79 -1.2 2,180,899 - rfbC Synpcc7942_2098 0.80 +0.2 2,180,946 + rfbC Synpcc7942_2098 0.89 -1.0 2,180,954 - -3.2 2,180,981 + +0.8 2,181,019 + -3.4 2,181,027 + -4.0 2,181,037 + -2.4 2,181,045 - -2.4 2,181,174 - rfbD Synpcc7942_2099 0.19 -5.1 2,181,179 + rfbD Synpcc7942_2099 0.20 -1.7 2,181,207 - rfbD Synpcc7942_2099 0.23 -1.7 2,181,328 + rfbD Synpcc7942_2099 0.37 -2.6 2,181,342 - rfbD Synpcc7942_2099 0.39 -2.4 2,181,382 + rfbD Synpcc7942_2099 0.43 -1.4 2,181,449 + rfbD Synpcc7942_2099 0.51 -1.7 2,181,553 + rfbD Synpcc7942_2099 0.63 -2.9 2,181,561 - rfbD Synpcc7942_2099 0.64 -1.8 2,181,614 + rfbD Synpcc7942_2099 0.70 -3.2 2,181,622 - rfbD Synpcc7942_2099 0.71 -1.4 2,181,710 + rfbD Synpcc7942_2099 0.81 -4.8 2,181,718 - rfbD Synpcc7942_2099 0.82 -1.4 2,181,727 + rfbD Synpcc7942_2099 0.83 -1.5 2,181,763 + rfbD Synpcc7942_2099 0.87 -2.8 2,181,764 + rfbD Synpcc7942_2099 0.87 -3.6 2,181,773 + rfbD Synpcc7942_2099 0.88 -2.5 2,181,786 - rfbD Synpcc7942_2099 0.90 -2.9 2,181,815 + -1.2 2,181,823 - -1.0 2,181,890 - -0.9 2,181,913 + -1.8 2,181,921 - -2.6 2,181,935 - -1.1 2,182,006 - rfbB Synpcc7942_2100 0.13 -0.5 2,182,007 + rfbB Synpcc7942_2100 0.13 -0.3 2,182,015 - rfbB Synpcc7942_2100 0.13 -0.4 2,182,015 - rfbB Synpcc7942_2100 0.13 +1.4 2,182,015 - rfbB Synpcc7942_2100 0.13 +0.1 2,182,038 - rfbB Synpcc7942_2100 0.16 -0.1 2,182,053 + rfbB Synpcc7942_2100 0.17 +0.0 2,182,093 - rfbB Synpcc7942_2100 0.21 -0.1 2,182,093 - rfbB Synpcc7942_2100 0.21 -0.8 2,182,107 - rfbB Synpcc7942_2100 0.22 -0.2 2,182,152 - rfbB Synpcc7942_2100 0.26 +0.3 2,182,202 - rfbB Synpcc7942_2100 0.31 +0.3 2,182,232 + rfbB Synpcc7942_2100 0.34 -0.3 2,182,258 + rfbB Synpcc7942_2100 0.36 +0.4 2,182,271 - rfbB Synpcc7942_2100 0.37 -1.4 2,182,278 + rfbB Synpcc7942_2100 0.38 +1.3 2,182,279 + rfbB Synpcc7942_2100 0.38 +0.8 2,182,286 - rfbB Synpcc7942_2100 0.39 -1.8 2,182,287 - rfbB Synpcc7942_2100 0.39 -0.5 2,182,362 - rfbB Synpcc7942_2100 0.46 -2.3 2,182,383 + rfbB Synpcc7942_2100 0.48 +0.2 2,182,395 + rfbB Synpcc7942_2100 0.49 -0.1 2,182,403 - rfbB Synpcc7942_2100 0.50 +0.1 2,182,405 - rfbB Synpcc7942_2100 0.50 -3.3 2,182,405 - rfbB Synpcc7942_2100 0.50 +1.2 2,182,457 - rfbB Synpcc7942_2100 0.55 -2.6 2,182,475 - rfbB Synpcc7942_2100 0.56 -1.0 2,182,489 - rfbB Synpcc7942_2100 0.58 +0.8 2,182,566 - rfbB Synpcc7942_2100 0.65 -0.3 2,182,571 + rfbB Synpcc7942_2100 0.65 +1.0 2,182,584 - rfbB Synpcc7942_2100 0.66 -0.5 2,182,584 - rfbB Synpcc7942_2100 0.66 -0.1 2,182,617 - rfbB Synpcc7942_2100 0.69 -1.9 2,182,641 - rfbB Synpcc7942_2100 0.72 -0.8 2,182,762 + rfbB Synpcc7942_2100 0.83 +0.4 2,182,921 - +0.2 2,182,968 - +1.0 2,182,991 + +0.2 2,182,991 + +2.1 2,182,991 + -0.2 2,182,999 - +1.0 2,183,003 + -0.1 2,183,005 + +0.5 2,183,005 + -1.2 2,183,013 - -0.4 2,183,013 - +0.2 2,183,046 - rfbA Synpcc7942_2101 0.12 -1.4 2,183,063 + rfbA Synpcc7942_2101 0.14 -0.2 2,183,115 + rfbA Synpcc7942_2101 0.20 -0.6 2,183,115 + rfbA Synpcc7942_2101 0.20 +0.4 2,183,115 + rfbA Synpcc7942_2101 0.20 +0.5 2,183,115 - rfbA Synpcc7942_2101 0.20 +0.4 2,183,115 - rfbA Synpcc7942_2101 0.20 -0.0 2,183,123 - rfbA Synpcc7942_2101 0.21 +0.4 2,183,123 - rfbA Synpcc7942_2101 0.21 +0.4 2,183,141 + rfbA Synpcc7942_2101 0.23 +0.3 2,183,177 + rfbA Synpcc7942_2101 0.27 +0.2 2,183,177 + rfbA Synpcc7942_2101 0.27 +0.5 2,183,207 - rfbA Synpcc7942_2101 0.30 +0.3 2,183,214 - rfbA Synpcc7942_2101 0.31 +0.7 2,183,238 + rfbA Synpcc7942_2101 0.34 -0.1 2,183,338 + rfbA Synpcc7942_2101 0.45 +0.5 2,183,383 - rfbA Synpcc7942_2101 0.50 -2.0 2,183,432 + rfbA Synpcc7942_2101 0.55 +0.6 2,183,432 + rfbA Synpcc7942_2101 0.55 -0.3 2,183,457 + rfbA Synpcc7942_2101 0.58 +1.4 2,183,466 + rfbA Synpcc7942_2101 0.59 -0.9 2,183,501 + rfbA Synpcc7942_2101 0.63 -1.3 2,183,513 - rfbA Synpcc7942_2101 0.65 -0.3 2,183,540 - rfbA Synpcc7942_2101 0.68 +0.0 2,183,549 + rfbA Synpcc7942_2101 0.69 +0.2 2,183,557 - rfbA Synpcc7942_2101 0.70 -2.1 2,183,574 + rfbA Synpcc7942_2101 0.72 +0.5 2,183,592 - rfbA Synpcc7942_2101 0.74 +0.2 2,183,609 + rfbA Synpcc7942_2101 0.75 -1.6 2,183,628 + rfbA Synpcc7942_2101 0.78 -1.0 2,183,628 + rfbA Synpcc7942_2101 0.78 -0.2 2,183,636 - rfbA Synpcc7942_2101 0.79 -0.7 2,183,636 - rfbA Synpcc7942_2101 0.79 -3.3 2,183,721 + rfbA Synpcc7942_2101 0.88 +1.2 2,183,740 - -0.4 2,183,746 + +0.9 2,183,754 - -0.1 2,183,763 + +0.1 2,183,771 - -0.6 2,183,804 - +0.1 2,183,813 - +0.1 2,183,827 - -0.8 2,183,841 + -0.1 2,183,849 + -0.0 2,183,849 - -1.2 2,183,862 + Synpcc7942_2102 0.10 +1.4 2,183,897 + Synpcc7942_2102 0.25 -0.2 2,183,905 - Synpcc7942_2102 0.28 +0.8 2,183,905 - Synpcc7942_2102 0.28 +0.2 2,183,905 - Synpcc7942_2102 0.28 +0.1 2,183,905 - Synpcc7942_2102 0.28 +0.8 2,183,924 + Synpcc7942_2102 0.36 +0.3 2,183,932 - Synpcc7942_2102 0.40 -2.0
Or see this region's nucleotide sequence