Experiment: KB with Veratrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2703 and Psyr_2704 are separated by 419 nucleotides Psyr_2704 and Psyr_2705 are separated by 73 nucleotides Psyr_2705 and Psyr_2706 are separated by 64 nucleotides
Psyr_2703: Psyr_2703 - conserved hypothetical protein, at 3,283,818 to 3,285,002
_2703
Psyr_2704: Psyr_2704 - UDP-galactose 4-epimerase, at 3,285,422 to 3,286,450
_2704
Psyr_2705: Psyr_2705 - Helix-turn-helix motif protein, at 3,286,524 to 3,287,135
_2705
Psyr_2706: Psyr_2706 - gamma-glutamyl-gamma-aminobutyrate hydrolase, at 3,287,200 to 3,287,949
_2706
Position (kb)
3285
3286
3287 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3284.482 kb on - strand, within Psyr_2703 at 3284.499 kb on - strand, within Psyr_2703 at 3284.650 kb on + strand, within Psyr_2703 at 3284.700 kb on + strand, within Psyr_2703 at 3284.852 kb on - strand, within Psyr_2703 at 3284.931 kb on + strand at 3284.931 kb on + strand at 3285.002 kb on + strand at 3285.002 kb on + strand at 3285.003 kb on - strand at 3285.020 kb on + strand at 3285.021 kb on - strand at 3285.109 kb on + strand at 3285.109 kb on + strand at 3285.109 kb on + strand at 3285.110 kb on - strand at 3285.110 kb on - strand at 3285.110 kb on - strand at 3285.110 kb on - strand at 3285.110 kb on - strand at 3285.110 kb on - strand at 3285.141 kb on + strand at 3285.141 kb on + strand at 3285.142 kb on - strand at 3285.142 kb on - strand at 3285.142 kb on - strand at 3285.142 kb on - strand at 3285.142 kb on - strand at 3285.245 kb on + strand at 3285.245 kb on + strand at 3285.287 kb on + strand at 3285.288 kb on - strand at 3285.449 kb on + strand at 3285.450 kb on - strand at 3285.450 kb on - strand at 3285.511 kb on + strand at 3285.550 kb on - strand, within Psyr_2704 at 3285.705 kb on - strand, within Psyr_2704 at 3285.864 kb on - strand, within Psyr_2704 at 3285.948 kb on - strand, within Psyr_2704 at 3286.051 kb on - strand, within Psyr_2704 at 3286.068 kb on + strand, within Psyr_2704 at 3286.068 kb on + strand, within Psyr_2704 at 3286.069 kb on - strand, within Psyr_2704 at 3286.069 kb on - strand, within Psyr_2704 at 3286.115 kb on + strand, within Psyr_2704 at 3286.115 kb on + strand, within Psyr_2704 at 3286.116 kb on - strand, within Psyr_2704 at 3286.252 kb on + strand, within Psyr_2704 at 3286.252 kb on + strand, within Psyr_2704 at 3286.252 kb on + strand, within Psyr_2704 at 3286.253 kb on - strand, within Psyr_2704 at 3286.253 kb on - strand, within Psyr_2704 at 3286.253 kb on - strand, within Psyr_2704 at 3286.525 kb on + strand at 3286.525 kb on + strand at 3286.525 kb on + strand at 3286.525 kb on + strand at 3286.526 kb on - strand at 3286.526 kb on - strand at 3286.612 kb on + strand, within Psyr_2705 at 3286.612 kb on + strand, within Psyr_2705 at 3286.612 kb on + strand, within Psyr_2705 at 3286.615 kb on + strand, within Psyr_2705 at 3286.747 kb on + strand, within Psyr_2705 at 3286.748 kb on - strand, within Psyr_2705 at 3286.855 kb on + strand, within Psyr_2705 at 3286.855 kb on + strand, within Psyr_2705 at 3286.855 kb on + strand, within Psyr_2705 at 3286.855 kb on + strand, within Psyr_2705 at 3286.855 kb on + strand at 3286.855 kb on + strand, within Psyr_2705 at 3286.856 kb on - strand, within Psyr_2705 at 3286.856 kb on - strand, within Psyr_2705 at 3286.856 kb on - strand, within Psyr_2705 at 3287.032 kb on + strand, within Psyr_2705 at 3287.032 kb on + strand, within Psyr_2705 at 3287.032 kb on + strand, within Psyr_2705 at 3287.032 kb on + strand, within Psyr_2705 at 3287.032 kb on + strand, within Psyr_2705 at 3287.033 kb on - strand, within Psyr_2705 at 3287.081 kb on + strand at 3287.081 kb on + strand at 3287.081 kb on + strand at 3287.082 kb on - strand at 3287.082 kb on - strand at 3287.082 kb on - strand at 3287.161 kb on + strand at 3287.161 kb on + strand at 3287.162 kb on - strand at 3287.219 kb on + strand at 3287.219 kb on + strand at 3287.219 kb on + strand at 3287.220 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Veratrine 10 mM remove 3,284,482 - Psyr_2703 0.56 -0.7 3,284,499 - Psyr_2703 0.57 +0.5 3,284,650 + Psyr_2703 0.70 +1.9 3,284,700 + Psyr_2703 0.74 +0.3 3,284,852 - Psyr_2703 0.87 -0.3 3,284,931 + -1.0 3,284,931 + -0.0 3,285,002 + +0.3 3,285,002 + +0.3 3,285,003 - -2.0 3,285,020 + +1.0 3,285,021 - +0.6 3,285,109 + +0.0 3,285,109 + -0.2 3,285,109 + -0.3 3,285,110 - -0.5 3,285,110 - +0.2 3,285,110 - -0.1 3,285,110 - +0.3 3,285,110 - +0.6 3,285,110 - +1.1 3,285,141 + -0.3 3,285,141 + -0.2 3,285,142 - -0.7 3,285,142 - -2.0 3,285,142 - +1.1 3,285,142 - +0.3 3,285,142 - +0.7 3,285,245 + -0.9 3,285,245 + -2.0 3,285,287 + -1.5 3,285,288 - -0.2 3,285,449 + -1.2 3,285,450 - -0.5 3,285,450 - +0.7 3,285,511 + +2.2 3,285,550 - Psyr_2704 0.12 +1.1 3,285,705 - Psyr_2704 0.28 -1.1 3,285,864 - Psyr_2704 0.43 -3.3 3,285,948 - Psyr_2704 0.51 +1.8 3,286,051 - Psyr_2704 0.61 -1.6 3,286,068 + Psyr_2704 0.63 +0.3 3,286,068 + Psyr_2704 0.63 +0.7 3,286,069 - Psyr_2704 0.63 +4.1 3,286,069 - Psyr_2704 0.63 -0.0 3,286,115 + Psyr_2704 0.67 +0.4 3,286,115 + Psyr_2704 0.67 -0.7 3,286,116 - Psyr_2704 0.67 -0.3 3,286,252 + Psyr_2704 0.81 -1.0 3,286,252 + Psyr_2704 0.81 +0.9 3,286,252 + Psyr_2704 0.81 -1.2 3,286,253 - Psyr_2704 0.81 -1.4 3,286,253 - Psyr_2704 0.81 -0.9 3,286,253 - Psyr_2704 0.81 +0.1 3,286,525 + -0.6 3,286,525 + +0.2 3,286,525 + -0.9 3,286,525 + +1.2 3,286,526 - -1.5 3,286,526 - -0.9 3,286,612 + Psyr_2705 0.14 +2.3 3,286,612 + Psyr_2705 0.14 +0.3 3,286,612 + Psyr_2705 0.14 -0.3 3,286,615 + Psyr_2705 0.15 -0.9 3,286,747 + Psyr_2705 0.36 -0.6 3,286,748 - Psyr_2705 0.37 -0.1 3,286,855 + Psyr_2705 0.54 +0.8 3,286,855 + Psyr_2705 0.54 -0.1 3,286,855 + Psyr_2705 0.54 -0.9 3,286,855 + Psyr_2705 0.54 +1.1 3,286,855 + +3.9 3,286,855 + Psyr_2705 0.54 +0.1 3,286,856 - Psyr_2705 0.54 -0.5 3,286,856 - Psyr_2705 0.54 +2.2 3,286,856 - Psyr_2705 0.54 -0.3 3,287,032 + Psyr_2705 0.83 +0.1 3,287,032 + Psyr_2705 0.83 +0.9 3,287,032 + Psyr_2705 0.83 -0.4 3,287,032 + Psyr_2705 0.83 +0.8 3,287,032 + Psyr_2705 0.83 +0.9 3,287,033 - Psyr_2705 0.83 +0.3 3,287,081 + -0.4 3,287,081 + -0.5 3,287,081 + -0.1 3,287,082 - +0.0 3,287,082 - -0.4 3,287,082 - +0.7 3,287,161 + +0.2 3,287,161 + -0.4 3,287,162 - -0.9 3,287,219 + -0.2 3,287,219 + +0.8 3,287,219 + -0.1 3,287,220 - -0.6
Or see this region's nucleotide sequence